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Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles

Yıl 2015, Cilt: 2 Sayı: 2, 45 - 51, 01.07.2015

Öz

Geotrichum fragrans (syn. Saprochaete suaveolens) can produce fruity aromas (pine-apple like) such as esters and alcohols, when cultivated in glucose containing liquid media. Consumers’ preferences for the use of natural products especially in foods and cosmetics have motivated the production of aroma chemicals by biotechnological means. In the present study, the volatile compounds of the G. fragrans during the 9 days cultivation in liquid media were accumulated by Headspace- Solid Phase Microextraction (HS-SPME) technique, and, analysed by gas chromatography/flame ionisation detector (GC/FID) and GC coupled to mass spectrometry (GC/MS), simultaneously. The main volatiles of the G. fragrans culture were determined as ethyl tiglate (8.5-74.6%), ethyl isovalerate (36.1-41.6%) and methyl isovalerate (0.1-10.9%) during the time course. Daily variation trends of the aroma volatiles were determined, where ethyl acetate was postulated as an intermediate metabolite in this bioconversion pathway

Kaynakça

  • Adams, R.P. (2001). Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass
  • Spectroscopy, USA: Allured Publishing.
  • Berger, R.G. (1995). Aroma Biotechnology, Springer-Verlag Berlin Heidelberg.
  • Berger, R.G. (2007). Flavours and Fragrances: Chemistry, Bioprocessing and Sustainability, Springer, Berlin. Damasceno, S., Cereda, M.P., Pastore, G.M., & Oliviera, J.G. (2003). Production of volatile compounds by
  • Geotrichum fragrans using cassawa waste water as a substrate. Process Biochemistry, 39, 411-414.
  • Winter, R. (2009). A Consumer's Dictionary of Food Additives, 7th Edition: Descriptions in Plain English of More
  • Than 12,000 Ingredients both Harmful and Desirable Found in Foods: New York, Three Rivers Press.
  • De Oliveira, S. M. M., Damasceno Gomes S., Sene, L., Machado Coelho, S. R., Barana, A. C., Cereda, M.P., Christ, D., and Piechontcoski, J. (2013). Production of 2-phenylethanol by Geotrichum fragrans, Saccharomyces cerevisiae and Kluyveromyces marxianus in cassava wastewater. Journal of Food Agriculture
  • & Environment, 11(2), 158-163.
  • Goldberg, I., & Williams, R. (1991). Biotechnology and Food Ingredients. USA: Van Nostrand-Reinhold.
  • Grondin, E., Sing, A. S. C., Caro, Y., de Billerbeck, G. M., François, J. M., and Petit, Thomas. (2015). Physiological and biochemical characteristics of the ethyl tiglate production pathway in the yeast Saprochaete suaveolens. Yeast, 32(1), 57-66.
  • Joulain, D., & König, W. A. (1998). The Atlas of spectra data of sesquiterpene hydrocarbons. Hamburg, E.B.- Verlag,
  • Koenig, W. A., Joulain, D., & Hochmuth. D. H. (2004). Terpenoids and related constituents of essential oils, MassFinder 3”, in Ed. Hochmuth D.H., Convenient and Rapid Analysis of GCMS, Hamburg, Germany.
  • McLafferty, F. W., & Stauffer, D. B. (1989). The Wiley/NBS registry of mass spectral data; New York, J. Wiley and Sons.
  • Mdaini, M., Gargouri, M., Hammami, M., Monser, L., & Hamdi, M. (2006). Production of Natural Fruity Aroma by Geotrichum candidum. Applied Biochemistry and Biotechnology, 128, 227-235.
  • Neto, R. S., Pastore, G. M. & Macedo, G.A. (2006). Biocatalysis and Biotransformation Producing γ- Decalactone. Food Science c, 69(9), 677-680.
  • Pinotti, T., Carvalho, P. M. B., Garcia, K. M. G., Silva, T. R., Hagler, A. N., Leite, & S. G. F. (2006). Media components and amino acid supplements influencing the production of fruity aroma by Geotrichum candidum. Brazilian Journal of Microbiology, 37, 494-498.
Yıl 2015, Cilt: 2 Sayı: 2, 45 - 51, 01.07.2015

Öz

Kaynakça

  • Adams, R.P. (2001). Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass
  • Spectroscopy, USA: Allured Publishing.
  • Berger, R.G. (1995). Aroma Biotechnology, Springer-Verlag Berlin Heidelberg.
  • Berger, R.G. (2007). Flavours and Fragrances: Chemistry, Bioprocessing and Sustainability, Springer, Berlin. Damasceno, S., Cereda, M.P., Pastore, G.M., & Oliviera, J.G. (2003). Production of volatile compounds by
  • Geotrichum fragrans using cassawa waste water as a substrate. Process Biochemistry, 39, 411-414.
  • Winter, R. (2009). A Consumer's Dictionary of Food Additives, 7th Edition: Descriptions in Plain English of More
  • Than 12,000 Ingredients both Harmful and Desirable Found in Foods: New York, Three Rivers Press.
  • De Oliveira, S. M. M., Damasceno Gomes S., Sene, L., Machado Coelho, S. R., Barana, A. C., Cereda, M.P., Christ, D., and Piechontcoski, J. (2013). Production of 2-phenylethanol by Geotrichum fragrans, Saccharomyces cerevisiae and Kluyveromyces marxianus in cassava wastewater. Journal of Food Agriculture
  • & Environment, 11(2), 158-163.
  • Goldberg, I., & Williams, R. (1991). Biotechnology and Food Ingredients. USA: Van Nostrand-Reinhold.
  • Grondin, E., Sing, A. S. C., Caro, Y., de Billerbeck, G. M., François, J. M., and Petit, Thomas. (2015). Physiological and biochemical characteristics of the ethyl tiglate production pathway in the yeast Saprochaete suaveolens. Yeast, 32(1), 57-66.
  • Joulain, D., & König, W. A. (1998). The Atlas of spectra data of sesquiterpene hydrocarbons. Hamburg, E.B.- Verlag,
  • Koenig, W. A., Joulain, D., & Hochmuth. D. H. (2004). Terpenoids and related constituents of essential oils, MassFinder 3”, in Ed. Hochmuth D.H., Convenient and Rapid Analysis of GCMS, Hamburg, Germany.
  • McLafferty, F. W., & Stauffer, D. B. (1989). The Wiley/NBS registry of mass spectral data; New York, J. Wiley and Sons.
  • Mdaini, M., Gargouri, M., Hammami, M., Monser, L., & Hamdi, M. (2006). Production of Natural Fruity Aroma by Geotrichum candidum. Applied Biochemistry and Biotechnology, 128, 227-235.
  • Neto, R. S., Pastore, G. M. & Macedo, G.A. (2006). Biocatalysis and Biotransformation Producing γ- Decalactone. Food Science c, 69(9), 677-680.
  • Pinotti, T., Carvalho, P. M. B., Garcia, K. M. G., Silva, T. R., Hagler, A. N., Leite, & S. G. F. (2006). Media components and amino acid supplements influencing the production of fruity aroma by Geotrichum candidum. Brazilian Journal of Microbiology, 37, 494-498.
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Articles
Yazarlar

Gökalp İşcan Bu kişi benim

Betül Demirci

Fatih Demirci

Kemal Başer

Yayımlanma Tarihi 1 Temmuz 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 2 Sayı: 2

Kaynak Göster

APA İşcan, G., Demirci, B., Demirci, F., Başer, K. (2015). Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles. Natural Volatiles and Essential Oils, 2(2), 45-51.