SİYAH HAVUÇ KALLUS KÜLTÜRÜNÜN ANTİOKSİDAN AKTİVİTESİ VE FENOLİK BİLEŞİK İÇERİĞİNİN BELİRLENMESİ

Öz

Kallus kültürü bir bitki doku kültürü yöntemi olup birçok sektörün ihtiyacı olan fenolik bileşiklerin üretimi için alternatif bir tekniktir. Bu çalışmada, siyah havuç (Daucus carota ssp. sativus var. atrorubens Alef.) bitkisinde kallus kültürü oluşturulması ve elde edilen kallusların antioksidan kapasitesi, toplam fenolik, flavonoid ve antosiyanin içeriklerinin belirlenmesi amaçlanmıştır. Ekstraktların antioksidan kapasiteleri serbest radikal giderme aktivitesi (DPPH: 2.2-diphenyl-1- picrylhydrazyl) ve demir indirgeme antioksidan gücü (FRAP) metotlarıyla
belirlenmiştir. Kallus indüksiyonu, steril koşullarda yetiştirilen bitkilerin hipokotil kısımlarının 2mg/L 2.4-D, 0.2mg/L BAP, 30g/L sükroz ve 2g/L phytagel içeren MS besi ortamında kültüre alınmasıyla (Murashige ve Skoog) sağlanmıştır. Siyah havuç kalluslarının yüksek miktarda fenolik bileşik içermesini yanında yüksek serbest radikal giderme aktivitesi (DPPH) ve demir indirgeme gücüne sahip olduğu belirlenmiştir. 

Anahtar Kelimeler

• Antioksidan Aktivite
• Antosiyanin
• Siyah Havuç
• Kallus Kültürü
• Flavonoid
• Fenolik Bileşik

DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE

Abstract

Callus culture, a method of plant tissue culture, is an alternative technique for the production of phenolic compounds that many industries need. In this study, antioxidant capacity, total phenolic, flavonoid and anthocyanin content of callus obtained from black carrot (Daucus carota ssp. Sativus var. Atrorubens alef.) were investigated. The antioxidant capacities of the extracts were determined by the free radical scavenging activity (DPPH) and the ferric reducing antioxidant power (FRAP) methods. The callus was derived from the MS medium (Murashige ve Skoog containing 2 mg/L 2.4-D, 0.2mg/L BAP, 30g/L sucrose and 2g/L phytagel using hypocotyl segments of plants grown in sterile conditions. The black carrot calli exhibited high DPPH free radical scavenging activity and FRAP activity in addition to high phenolic compound contents.

Keywords

• Antioxidant Activity
• Anthocyanin
• Black Carrot
• Callus Culture
• Flavonoid
• Phenolic Compound

Kaynakça

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