TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONU

Yıl 2021, Cilt: 29 Sayı: 1, 86 - 96, 30.04.2021

Gülçin Özevci Oguz Akpolat Hakan Ayhan Fatma Ayhan

https://doi.org/10.31796/ogummf.797983

Öz

Hidrojen peroksit tekstil endüstrisinde ağartıcı olarak kullanılan kuvvetli bir oksidant kimyasaldır ve çevreye verilmeden önce sulardan giderilmesi önemlidir. Peroksidaz (YTP) enziminin oksitleyici substratı hidrojen peroksittir. Yapılan bu çalışmada, hidrojen peroksitin giderilmesinde YTP enziminin çapraz bağlı agregatlar şeklinde immobilize edilmiştir ve çapraz bağlayıcı ajan olarak glutaraldehit (GA) kullanılmıştır. Serbest ve agregat formundaki enzimlerin kinetik parametreleri hesaplanmıştır. Serbest peroksidaz enziminin kinetic sabitleri hesaplandığında doygunluk sabiti 0,06965 mmol H2O2/L ve, maksimum özgül hız sabiti νm değeri ise 0.0232 mmol H2O2/L dk olarak bulunmuştur. Boyasız hidrojen peroksitli atık suda YTP-ÇBEA’nın (Çapraz Bağlı Enzim Agregatları) Km doygunluk sabiti 0,00625 mmol H2O2/L, maksimum özgül hız sabiti νm değeri ise 0.0699 x 10 -3 mmol H2O2/L dk olarak hesaplanmıştır. Oksidasyon işlemlerinde YTP-ÇBEA ile 15 dk içinde % 100 H2O2 uzaklaştırma sağlanmıştır. H2O2 (0,02M) içeren atık suda giderimin beş dakikada tamamlanmıştır. Ölçek büyütme yaklaşımında Euler çözüm algoritması kullanılarak veriler değerlendirilmiş ve kinetik sabitlerin değiştiği etkinlik faktör aralığında H2O2 giderimi hesaplanmıştır. Tepkime hızının doğrusal olarak değiştiği kabul edilerek elde edilen agregat formundaki enzimle ölçek büyütülmesi sırasında oluşacak aktivite farklılaşmaları % 125-% 50 aralığında belirlenmiş olup, bunlara bağlı etkinlik faktörleri dikkate alınarak endüstriyel boyutta tasarım modellenmiştir. Büyütülen tepkime hacminde oluşacak derişimler simule edilmiştir. Elde edilen sonuçlar YTP-ÇBEA’ların zaman ve maliyet açısından serbest enzime göre daha avantajlı olduğunu göstermiştir.

Anahtar Kelimeler

Peroksidaz, Çapraz Bağlı Enzim Agregatları, Taşıyıcısız Enzim İmmobilizasyonu, Modelleme

Destekleyen Kurum

Muğla Sıtkı Koçman Üniversitesi

Proje Numarası

2011-51

MODELLING AND SIMULATION OF SCALE-UP DESIGN FOR THE REMOVAL OF HYDROGEN PEROXIDE WITH HRP-CLEA IN TEXTILE WASTEWATER

Öz

Hydrogen peroxide is a strong oxidant chemical used as a bleach in the textile industry and it is important to remove it from water before being released to the environment. The oxidizing substrate of peroxidase (HRP) enzyme is hydrogen peroxide. In this study, cross-linked aggregates of HRP enzyme were used to remove hydrogen peroxide and glutaraldehyde (GA) was used as a crosslinking agent. The saturation constant was 0,06965 mmol H2O2/L and maximum specific rate constant was found as 0.0699 x 10 -3 mmol H2O2/L min. Saturation constant, Km of HRP-CLEA (Cross Linked Enzyme Aggregates) was found as 0,06965 mmol H2O2 / L, and maximum specific rate constant value, νm, was calculated as 0.0699 x 10 -3 mmol H2O2 / L sec. In oxidation processes, 100% H2O2 removal was achieved in 15 minutes with HRP-CLEA. . The removal of the waste water containing H2O2 (0.02M) was completed in five minutes. In the scale-up approach, the data was evaluated using the Euler solution algorithm and the H2O2 removal in the efficiency factor range where the kinetic constants change was calculated. Assuming the reaction rate changes linearly, the activity variations that will occur during scaling up with the enzyme in aggregate form were determined in the range of 125% -50%, and the industrial-scale design was modelled by taking into account the activity factors. In scaling up, concentrations that will occur in the increased reaction volume have been simulated. The results obtained showed that HRP-CLEAs are more advantageous than free enzyme in terms of time and cost.

Anahtar Kelimeler

Peroxidase, Cross-Linked Enzyme Aggregates, Carrier-Free Enzyme Immobilization

Proje Numarası

2011-51

Kaynakça

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