The Use of Molasses in Decolorization by Purple Non-Sulfur Bacteria

Abstract

It is well known that waste water discharged from textile industry which contains dye stuff causes environmental pollution. In these aspects, decolorization processes of textile waste water possess much importance. Reactive Black 5 (RB5), which is widely used in textile industry, is a diazo reactive dye. In this study the decolorization ability of purple non-sulphur bacteria strains Rhodopseudomonas palustris, Rhodobacter sphaeroides and Rhodoplanes piscinae in the presence of RB5 and sugar beet molasses as a co-substrate, which is a by-product of sugar production, is investigated. The dye concentration was adjusted as 100 mg/L in culture media. The dye decolorization in media containing molasses as co-substrate was measured spectrophotometrically. The dye degradation was supported by FT-IR analysis. The decolorization percentages in media containing 100 mg/L RB5 were determined as 86.80, 82.37 and 84.06 % by R. piscinae 51ATA, R. palustris DSMZ127 and R. spheroides DSMZ 5864, respectively. It was considered that the dye was used by the bacteria since the aromatic rings of the dye could not be determined by FT-IR analysis on the surface of bacteria. It was also determined from FT-IR analysis that the peaks belonging to the nitrogen in the azo bond were removed as well as the other peaks of the dye resulting in the complete mineralization of the dye by the bacteria

Keywords

• Textil dye
• Reactive Black 5
• Decolorization
• Photosyntetic bacteria
• purple non sulphur bacteria

Melasın Mor Kükürtsüz Bakteriler Tarafından Dekolorizasyonda Kullanılması

Öz

Toksik özelliklere sahip tekstil endüstrisinde kullanılan boyar maddelerin deşarj sularının çevre kirliliğine neden olduğu bilinmektedir. Bu bağlamda boyarmadde içeren tekstil endüstrisi atıksularının renk giderim süreçleri ekolojik açıdan büyük önem kazanmaktadır. Reactive Black 5 (RB5) boyası, tekstil endüstrisinde yaygın olarak kullanılan reaktif boyalardan biri olup diazo bir boyadır. Bu çalışmada, mor kükürtsüz fotosentetik bakteriler olan Rhodopseudomonas palustris, Rhodobacter sphaeroides ve Rhodoplanes piscinae suşlarının RB5 boyasını dekolorizasyon yeteneği, şeker atığı olan melas yardımcı substratı varlığında araştırılmıştır. Boya konsantrasyonu 100 mg/L olacak şekilde besiyerlerine ilave edilmiştir. Yardımcı substratın kullanıldığı ortamda boyanın dekolorizasyonu, spektrofotometrik olarak takip edilmiştir. FTIR analizleri ile boya gideriminin tespiti desteklenmiştir. 100 mg/L RB5 boyasını içeren besiyerinde R. piscinae 51ATA suşunun %86,80 oranında, R. palustris DSMZ127 suşunun %82,37 oranında ve R. sphaeroides DSMZ 5864 suşunun ise %84,06 oranında dekolorizasyon gerçekleştirdiği tespit edilmiştir. FT-IR analizinde, bakteri yüzeyinde boyanın aromatik halkalarının görülmemesi boyanın bakteri tarafından kullanıldığını düşündürmüştür. Boyanın FT-IR spektrumlarından, diğer pikler gibi azo bağlarındaki azotun da dahil olduğu azo piklerinin uzaklaştığı, boyanın bakteri tarafından tamamen mineralize edildiği tespit edilmiştir.

Anahtar Kelimeler

• Tekstil boyası
• Reactive black 5
• Dekolorizasyon
• Fotosentetik bakteriler
• mor kükürtsüz bakteriler

Kaynakça

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