Phylogenetic Analysis of Some Taxa Belonging To The Family Lamiaceae in Bitlis Province Using RAPD-PCR Technique

Abstract

In this study, the relationship between 54 taxa of 21 genera belonging to the family Lamiaceae, which grow naturally in Bitlis province, was examined. Genetic similarities between taxa were determined by RAPD - PCR technique. According to the results, the genera Phlomis L., Lamium L., Ballota L., Stachys L. and Sideritis L. in the subfamily of Lamioideae were supported by the morphological systematics; whereas, the genera Marrubium L. separated from the group. It was observed that taxa belonging to the genera Nepeta L., Lallemantia Fisch. & C.A. Mey, Melissa L., Prunella L., Origanum L., Satureja L., Clinopodium L., Cyclotrichium (Boiss.) Manden. & Scheng., Mentha L. and Salvia L. from the subfamily Nepeteoideae supported morphological system, but Ziziphora clinopodioides Lam. taxa showed difference. According to similarity matrix, the similarity was found mostly between Clinopodium vulgare L. subsp. arundanum (Boiss.) Nyman and Clinopodium graveolens subsp. rotundifolium (Pers.) Govaerts with the rate of 0.955 and between Salvia verticillata L. subsp. verticillata and Salvia verticillata subsp. amasiaca (Freyn & Bornm.) Bornm. with the rate of 0.934.

Keywords

• Molecular phylogeny
• Taxonomic classification
• Bitlis
• Lamiaceae
• RAPD-PCR

Bitlis İli Lamiaceae Familyasına Ait Bazı Taksonların RAPD-PCR Tekniği Kullanılarak Filogenetik Analizi

Öz

Bu çalışmada, Bitlis ilinde doğal olarak yetişen Lamiaceae familyasına ait 21 cinse ait 54 takson arasındaki ilişki incelenmiştir. Taksonlar arasındaki genetik benzerlikler RAPD - PCR tekniği ile belirlendi. Elde edilen sonuçlara göre, Lamioideae alt familyasında yer alan Phlomis L., Lamium L., Ballota L., Stachys L. ve Sideritis L. cinslerinin morfolojik sistematiği ile desteklendiği; Oysa ki Marrubium L. cinsi ise gruptan ayrılmıştır. Nepeteoideae alt familyasından Nepeta L., Lallemantia Fisch. & C.A. Mey, Melissa L., Prunella L., Origanum L., Satureja L., Clinopodium L., Cyclotrichium (Boiss.) Manden. & Scheng., Mentha L. ve Salvia L. cinsine ait taksonların morfolojik sistemi desteklediği, ancak Ziziphora clinopodioides Lam. taksonlarının farklılık gösterdiği gözlendi. Benzerlik matrisine göre benzerlik en çok 0.955 oranı ile Clinopodium vulgare L. subsp. arundanum (Boiss.) Nyman ve Clinopodium graveolens subsp. rotundifolium (Pers.) Govaerts ve 0.934 oranı ile Salvia verticillata L. subsp. verticillata ve Salvia verticillata subsp. amasiaca (Freyn & Bornm.) Bornm. arasında bulunmuştur.

Anahtar Kelimeler

• Moleküler filogeni
• Taksonomik sınıflandırma
• Bitlis
• Lamiaceae
• RAPD-PCR

Kaynakça

1. Al-Qurainy F., Khan S., Nadeem M., Tarroum M., Gaafar A. Selection of DNA barcoding loci for Nepeta deflersiana Schweinf. ex Hedge from chloroplast and nuclear DNA genomes. Genet. Mol. Res. 2014; 13(1): 1144-1151.
2. Al-Rawashdeh IM. Molecular taxonomy among Mentha spicata, Mentha longifolia and Ziziphora tenuior populations using the RAPD technique. JJBS 2011; 4(2): 63- 70.
3. Apostolova E., Anachkov G., Todorov K., Dyulgerova ID., Mladenov R., Stoyanov P., Yahubyan G., Naimov S. Genetic variability of chosen Bulgarian Mentha Species. Compt. Rend. Acad. Bulg. Sci. 2016; 69: 725-730.
4. Bendiksby M., Thorbek L., Scheen AC., Lindqvist C., Ryding O. An updated phylogeny and classification of Lamiaceae subfamily Lamioideae. Taxon 2011; 60(2): 471–484.
5. Bozarı S., Agar G., Yanmış D. Chemical content, and toxic effects of essential oil of Origanum vulgare L. ssp vulgare against to Zea mays seedlings. Journal of Essential Oil Bearing Plants 2014; 17(1): 67-77.
6. Bui ST., Khong TT., Ho VT. Genetic diversity and characterization of Pseuderanthemum latifolium By RAPD and ISSR molecular markers. Journal of Animal & Plant Sciences 2022; 32(1):78-83.
7. Chiang YC., Huang BH., Liao PC. Diversification, biogeographic pattern, and demographic history of taiwanese scutellaria species inferred from nuclear and chloroplast DNA. Plos One 2012; 7(11): 1-15.
8. Davis PH. Flora of Turkey and The East Aegean Islands. Edinburgh: Edinburgh Univ. Press Vol. 1-9; 1965-1985

9. Davis PH. Flora of Turkey and The East Aegean Islands. Edinburgh: Edinburgh Univ. Press. Vol.7; 1982
10. Davis PH., Mill RR., Tan K. (eds.). Flora of Turkey and The East Aegean Islands. (supplement 1) Edinburgh: Edinburgh Univ. Press., Vol.10; 1988
11. Dirmenci T., Dündar E., Deniz G., Arabaci T., Martin E. Morphological, karyological and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle. Turk J Bot. 2010; 34: 159-170.
12. Drew BT., Sytsma KJ. Phylogenetics, biogeography, and staminal evolution in the tribe Mentheae (Lamiaceae). American Journal of Botany 2012; 99(5): 933–953.