Phytochemical Composition, Antioxidant And Antimicrobial Activities of Essential Oils of Pinus sylvestris var. hamata

Öz

Pinus slyvestris L., which belongs to the Pinaceae family, is one of valuable forest trees. Pine oils are used as a flavoring in beverages and food and as a fragrance in cosmetics. Pinus sylvestris var hamata was collected from the Central Black Sea Region of Turkey and its needles were dried in the shade. Essential oils (EOs) was generated by hydrodistillation and Gas chromatography/mass spectrophotometry (GC-MS/MS) was used to identifiy the EOs compounds. β-Pinene (18.70%), α-Pinene (15.62%), Germacrene (12.53%), Caryophyllene (11.35%) and Limonene (3.62%) were major constituents. DPPH• ABTS+• scavenging and FRAP tests were performed for antioxidant analysis of EOs. Morever, antibacterial effect was investigated by disk diffusion assay using Bacillus cereus, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus and. EOs exhibited good antibacterial activities against the tested bacteria, except for Salmonella typhimurium. In addition EOs revealed the significant activity on tested assays. As results, P. sylveris var. hamata essential oil has possible to be utilized in the pharmaceutical and food industries

Anahtar Kelimeler

• antibacterial test
• antioxidant analysis
• essential oil
• Pinus sylvestris var. hamata

Pinus sylvestris var. hamata Uçucu Yağlarının Fitokimyasal Bileşimi, Antioksidan ve Antimikrobiyal Aktiviteleri

Öz

Çamgiller familyasına ait olan Pinus slyvestris L., değerli orman ağaçlarından biridir. Çam yağları, içecek ve yiyeceklerde tatlandırıcı olarak ve kozmetikte koku olarak kullanılır. Pinus sylvestris var. hamata Türkiye'nin Orta Karadeniz Bölgesi'nden toplanmış ve iğneleri gölgede kurutulmuştur. Uçucu yağlar (EO'lar) hidrodistilasyonla üretildi ve EO bileşiklerini tanımlamak için Gaz kromatografisi/kütle spektrofotometrisi (GC-MS/MS) kullanıldı. β-Pinene (%18,70), α-Pinene (%15,62), Germacrene (%12,53), Karyofilen (%11,35) ve Limonen (%3,62) ana bileşenlerdi. EO'ların antioksidan analizi için DPPH•, ABTS+• süpürme ve FRAP testleri yapıldı. Ayrıca, antibakteriyel etki, Bacillus cereus, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae ve Staphylococcus aureus ve kullanılarak disk difüzyon testi ile araştırılmıştır. EO'lar, Salmonella typhimurium dışında test edilen bakterilere karşı iyi antibakteriyel aktiviteler sergiledi. Ek olarak, EO'lar, test edilen deneylerde önemli aktiviteyi ortaya çıkardı. Sonuç olarak, P. sylveris var. hamata esansiyel yağının ilaç ve gıda endüstrisinde kullanılması mümkündür.

Anahtar Kelimeler

• antibakteriyel test
• antioksidan analiz
• esansiyel yağ
• Pinus sylveris var. hamata

Kaynakça

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