Salisilik Asit Orkidelerin Tozlaşma Sonrası Sürecinde Absisik Asit ve Gibberellik Asit ile Birlikte Etkilidir

Öz

Bu çalışmanın amacı orkide stigmalı ovaryumlarında uyumlu (Intraspesifik = ISP) ve uyumsuz tozlaşmada (Intergeneric = IGP) absisik asit (ABA), Jasmonik asit (JA) ve Salisilik asitin (SA) endojen hormon aktivitelerini belirlemektir. ISP tozlaşma deneyleri Himantoglossum robertianum çiçekleri arasında yapılmıştır. IGP deneylerinde tozlaşma, Himantoglossum robertianum ve Orchis italica çiçekleri arasında gerçekleştirildi. Bunun için O. italica'dan alınan polinariumlar, H. robertianum'un çiçek stigmalarına özel iğnelerle uygulanmıştır. Her iki tozlaşma tipine ait stigmalı ovaryumlar toplam 10 gün boyunca her gün için ayrı ayrı alınmış ve LC-MS/MS ile kantitatif endojen hormon analizleri yapılmıştır. Sonuçta SA hormonu ilk kez hem ISP'de hem de IGP'de tespit edildi ancak yalnızca ISP değerleri anlamlı çıkmıştır. Yapılan istatistiksel analizde SA'nın sadece ISP'de anlamlı olduğu, ABA değerlerinin ise hem ISP'de hem de IGP'de oldukça anlamlı olduğu görüldü. ISP9 da yüksek önem gösterdi. Ayrıca ABA değerleri, özellikle IGP deneylerinde genellikle SA değerlerinden yüksekti. Sonuçlar aynı zamanda ilk kez ovaryum endojen hormon aktivitelerinin önemini de vurguladı. Analizlerde JA tespit edilmemiştir

Anahtar Kelimeler

• himantoglossum
• Hormon
• Orkide
• Post-pollination fenomen
• Çiçek

Saliciylic Acid is also Effective Along with Abscisic Acid and Gibberellic Acid in the Orchid Post-Pollination Process

Abstract

The aim of this study is to determine the endogenous hormone activities of abscisic acid (ABA), Jasmonic acid (JA) and Salicylic acid (SA) in compatible (Intraspecific = ISP) and incompatible pollination (Intergeneric = IGP) in stigmatic ovaries during orchid post-pollination process. It was designed to be Himantoglossum robertianum in ISP experiments and Himantoglossum robertianum and Orchis italica in IGP experiments. Polynariums taken from O. italica have been applied with needles to the flower stigmas of H. robertianum. Ovaries with stigma from both pollination types were taken separately for each day for a total of 10 days and quantitative endogenous hormone analyzes have been performed by LC-MS/MS. After all, SA hormone has been detected for the first time in both ISP and IGP, but only ISP values were significant. The statistical analysis revealed that while SA is significant in only ISP, ABA values were found to be highly significant in both ISP and IGP. ISP9 also showed high significance. Additionally, ABA values were generally higher than SA values, especially in IGP experiments. The results also emphasized the importance of ovarian endogenous hormone activities for the first time. JA was not detected in the analysis.

Keywords

• Himantoglossum
• hormone
• orchid
• post-polinasyon phenomena
• flower

Kaynakça

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