Carduus nutans L.’ın LC-MS/MS ile Fitokimyasal Analizi, Antioksidan, Antidiyabetik, Antiglokom ve Anti-Alzheimer Etkileri

Öz

Carduus nutans L. (Asteraceae), halk arasında “Eşek dikeni” olarak bilinir ve kökleri cüzzam ve kabızlık tedavisinde, yaprakları ise sıtma tedavisinde kullanılmaktadır. Yapılan çalışmada Carduus nutans metanol ekstraktı (MECN) antioksidan aktivitesi ve enzim inhibisyon etkileri spektrofotometrik olarak belirlenmiştir. Antioksidan deneylerinden üçü Fe3+, Cu2+ ve Fe3+-TPTZ indirgeme deneyi olup sırasıyla 20 μg/mL’de ki absorbansları λ700: 0,470, λ450: 0,521 ve λ593: 0,671’dir. Diğer antioksidan deneyleri ise DPPH, ABTS ve DMPD radikalleri süpürme deneyi olup IC50 değerleri sırasıyla 115,52 μg/mL, 37,71 μg/mL, 85,47 μg/mL’dir. MECN’nin asetilkolinesteraz (AChE), α-glikozidaz (α-gli) ve karbonik anhidraz I-II (KA I-II) enzimlerine karşı inhibisyon etkilerinin IC50 değerleri sırasıyla 3,28 μg/mL, 8,37 μg/mL, 18,74 μg/mL ve 21,83 μg/mL’dir. MECN’nin toplam fenolik madde miktarı 153,83 μg/g GAE, toplam flavonoid madde miktarı ise 221,0 μg/g KE’dir. Ayrıca MECN’nin LC-MS/MS analizi yapılmış 7,07 µg/g fumarik asit, 2,72 µg/g kinik asit, 1,09 µg/g klorojenik asit majör madde olarak tespit edilmiştir. C. nutans metanol ekstraktı yüksek antioksidan aktiviteye sahiptir ve AChE, α-gli ve KA I-II enzimlerine karşı iyi bir inhibisyon etkisine sahiptir.

Anahtar Kelimeler

• Carduus nutans
• Antioksidan aktivite
• Enzim inhibisyonu
• LC-MS/MS analizi

Phytochemical Analysis of Carduus nutans L. via LC-MS/MS, Antioxidant, Antidiabetic, Antiglaucoma and Anti-Alzheimer's Effects

Öz

Carduus nutans L. (Asteraceae) is popularly known as "Eşek dikeni" and its roots are used in the treatment of leprosy and constipation, and its leaves are used in the treatment of malaria. In the study, antioxidant activity and enzyme inhibition effects of Carduus nutans methanol extract (MECN) were determined spectrophotometrically. Three of the antioxidant assays are Fe3+, Cu2+ and Fe3+-TPTZ reduction experiments, and their absorbances at 20 μg/mL are λ700:0,470, λ450: 0,521, and λ593: 0,671, respectively. Other antioxidant assays are DPPH, ABTS and DMPD radical scavenging experiments and IC50 values are 115.52 μg/mL, 37.71 μg/mL, and 85.47 μg/mL, respectively. The IC50 values of the inhibitory effects of MECN against acetylcholinesterase (AChE), α-glucosidase (α-gly) and carbonic anhydrase I-II (CA I-II) are 3.28 μg/mL, 8.37 μg/mL, 18.74 μg/mL, and 21.83 μg/mL, respectively. The total phenolic substance amount of MECN is 153.83 μg/g GAE, and total flavonoid substance amount is 221.0 μg/g QE. Additionally, LC-MS/MS analysis of MECN was performed and 7.07 µg/g fumaric acid, 2.72 µg/g quinic acid and 1.09 µg/g chlorogenic acid were determined as majör components. C. nutans methanol extract has high antioxidant activity and a good inhibition effect against AChE, α-gly, and CA I- II enzymes.

Anahtar Kelimeler

• Carduus nutans
• Antioxidant activity
• Enzym inhibition
• LC-MS/MS analysis

Kaynakça

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