Halk Elinde Yetiştirilen Bazı İvesi ve Akkaraman Koyun Irklarında Calpastatin Gen Polimorfizminin Büyüme Özellikleri ile İlişkisi

Öz

Bu çalışmada Osmaniye ilinde halk elinde ıslah projesi kapsamında yetiştiriciliği yapılan İvesi ve Akkaraman koyunlarında Kalpastatin geni (CAST) polimorfizminin belirlenmesi amaçlanmıştır. Çalışma 5 farklı işletmede yetiştirilen 20 baş İvesi koyundan ve yine 5 farklı işletmede yetiştirilen 19 baş Akkaraman koyundan alınan toplam 39 kan örneği üzerinde yürütülmüştür. PCR-RFLP yönteminin kullanıldığı çalışmada, CAST geni için İvesi ve Akkaraman koyun ırklarına ait genomik DNA örneklerinden elde edilen 622 bç uzunluğundaki PCR amplikonlarının MspI restriksiyon enzimi ile kesimi sonucunda; homozigot MM alleli için 336 ve 286 bç uzunluğunda iki adet bant, homozigot NN alleli için 622 bç uzunluğunda tek bir bant ve heterozigot MN allelleri için ise 622, 336 ve 286 bç uzunluğunda üç adet bant gözlenmiştir. Akkaraman ırkında M ve N allellerinin frekansı sırasıyla 0.82 ve 0.18 olarak hesaplanırken, İvesi ırkında M ve N frekansları sırasıyla 0.75 ve 0.25 olarak hesaplanmıştır. MM, MN ve NN genotiplerinin frekansları ise sırasıyla 0.60, 0.30 ve 0.10 olarak bulunmuştur. İvesi ırkının aksine Akkaraman koyunlarında sadece MM ve MN genotipleri gözlenmiş ve frekansları sırasıyla 0,63 ve 0,37 olarak hesaplanmıştır. Akkaraman popülasyonunda Hardy-Weinberg dengesinde sapma önemsiz bulunurken, İvesi koyununda önemli bulunmuştur. İlişki analizi İvesi ırklarında CAST geni polimorfizmi ile 90. gün ağırlığı arasında önemli bir ilişki olduğunu (P<0.05) ortaya koyarken, Akkaraman ıkında bu ilişki bulunamamıştır (P<0.05).

Anahtar Kelimeler

• CAST geni
• İvesi koyunu
• Akkaraman koyunu
• Polimorfizm
• Genotip frekansı

Relationship of Calpastatin Gene Polymorphism with Growth Traits in Some Locally Reared Awassi and Akkaraman Sheep Breeds

Abstract

In this study, it was aimed to determine the polymorphism of Calpastatin gene (CAST) in Awassi and Akkaraman sheep which were reared in Osmaniye province within the scope of National Sheep Breeding Project in the Hand of the Public. The study was carried out on a total of 39 blood samples taken from 20 head Awassi sheep and 19 head Akkaraman sheep reared in 5 different farms. In the study in which PCR-RFLP method was used, 622 bp long PCR amplicons obtained from genomic DNA samples of Awassi and Akkaraman sheep breeds for CAST gene were digested with MspI restriction enzyme; Two bands of 336 and 286 bp in length for homozygous MM allele, a single band of 622 bp in length for homozygous NN allele and three bands of 622, 336 and 286 bp in length for heterozygous MN alleles were observed. The frequencies of M and N alleles in Akkaraman breed were 0.82 and 0.18, respectively, while the frequencies of M and N in Awassi breed were 0.75 and 0.25, respectively. The frequencies of MM, MN and NN genotypes were 0.60, 0.30 and 0.10, respectively. Contrary to Awassi breed, only MM and MN genotypes were observed in Akkaraman sheep and their frequencies were calculated as 0.63 and 0.37, respectively. While deviation in Hardy-Weinberg equilibrium was found to be insignificant in Akkaraman population, it was found to be significant in Awassi sheep. The association analysis revealed that there was a significant association (P<0.05) between CAST gene polymorphism and 90th day weight in Awassi breeds, while this association was not found in Akkaraman breed (P<0.05).

Keywords

• CAST gene
• Awassi sheep
• Akkaraman sheep
• Polymorphism
• Genotype frequencies

Kaynakça

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