Heracleum platytaenium Boiss. Uçucu Yağının Elma Kara Leke ve Monilya Hastalığına Neden Olan Funguslara Karşı Antifungal Aktivitesi

Yıl 2025, Cilt: 8 Sayı: 4, 1882 - 1892, 16.09.2025

Işıl Saraç Sivrikaya , Abdullah Güller , Bekir Tosun

https://doi.org/10.47495/okufbed.1650326

Öz

Bu çalışma, Türkiye'ye özgü endemik bir tür olan Heracleum platytaenium Boiss.'in uçucu yağının Monilinia spp. ve Venturia inaequalis gibi fitopatojenik funguslar üzerindeki antifungal aktivitesini incelemektedir. Uçucu yağ, bitkinin tam çiçeklenme döneminde toplanan meyvelerinden hidrodistilasyon yöntemiyle elde edilmiştir. Çalışmada, Monilinia spp. ve V. inaequalis izolatları üzerinde farklı konsantrasyonlarda (5 µL, 10 µL, 20 µL) uçucu yağın antifungal etkisi in vitro olarak değerlendirilmiştir. Antifungal etkinlik, miselyal büyüme inhibisyonu yüzdesi ve istatistiksel analizlerle belirlenmiştir. Sonuçlar, uçucu yağın her iki patojen üzerinde doza bağlı olarak değişen inhibisyon etkisi gösterdiğini ortaya koymuştur. Monilinia spp. için en yüksek inhibisyon oranı %48,8, V. inaequalis için ise %46,6 olarak kaydedilmiştir. Ekstraksiyon yılına bağlı olarak antifungal etkinlikte farklılıklar gözlemlenmiş, 2021 yılına ait uçucu yağ örneklerinin 2022 yılına göre daha yüksek inhibisyon oranına sahip olduğu belirlenmiştir. Çalışmanın bulguları, H. platytaenium uçucu yağının önemli bir biyopestisit potansiyeline sahip olduğunu ve fitopatojenlere karşı doğal bir mücadele yöntemi olarak değerlendirilebileceğini göstermektedir. Bu sonuçlar, bitkisel bazlı antifungal ajanların sürdürülebilir tarım uygulamalarındaki önemini vurgulamakta ve kimyasal fungisitlere alternatif olabilecek yeni yaklaşımlara ışık tutmaktadır.

Anahtar Kelimeler

Heracleum platytaenium , uçucu yağ , Monilinia spp. , Venturia inaequalis , antifungal aktivite

Antifungal Activity of Heracleum platytaenium Essential Oil Against Fungi Causing Apple Scab and Monilia Disease

Öz

This study investigates the antifungal activity of the essential oil of Heracleum platytaenium Boiss., an endemic species in Türkiye, against the phytopathogenic fungi Monilinia spp. and Venturia inaequalis. The essential oil was obtained by hydrodistillation from the fruits collected at the full flowering stage. The antifungal effects of different concentrations (5 µL, 10 µL, 20 µL) of the essential oil were evaluated in vitro on Monilinia spp. and V. inaequalis isolates. Antifungal activity was determined based on the percentage inhibition of mycelial growth and statistical analyses. The results showed that the essential oil exhibited a dose-dependent inhibitory effect on both pathogens. The highest inhibition rates were recorded as 48.8% for Monilinia spp. and 46.6% for V. inaequalis. Differences in antifungal activity were observed depending on the extraction year, with the 2021 essential oil samples showing higher inhibition rates compared to those from 2022. The findings suggest that H. platytaenium essential oil has significant potential as a biopesticide and may serve as a natural control agent against phytopathogens. These results highlight the importance of plant-based antifungal agents in sustainable agricultural practices and offer new insights into alternatives to chemical fungicides.

Anahtar Kelimeler

Heracleum platytaenium , essential oil , Monilinia spp. , Venturia inaequalis , antifungal activity

Kaynakça

• Akçin A., Seyis F., Akçin TA., Çaycı YT., Çoban AY. Chemical composition and antimicrobial activity of the essential oil of endemic Heracleum platytaenium Boiss. from Turkey. Journal of Essential Oil Bearing Plants 2013; 16(2): 166-171.
• Bahadori MB., Dinparast L., Zengin G. The genus Heracleum: A comprehensive review on its phytochemistry, pharmacology, and ethnobotanical values as a useful herb. Comprehensive Reviews in Food Science and Food Safety 2016; 15(6): 1018-1039.
• Bayan Y., Yılar M., Onaran A. Antifungal activity and chemical composition of the essential oil of Heracleum platytaenium Boiss's. Egyptian Journal of Biological Pest Control 2016; 26(2): 237-240.
• Bayan Y., Yılar M., Onaran A. Heracleum platytaenium Boiss. ve Myrtus communis L. bitki uçucu yağlarının Alternaria solani Ell. and G. Martin ve Monilia laxa Aderh. and Ruhl. (Honey) üzerine antifungal aktivitesinin araştırılması. Akademik Ziraat Dergisi 2017; 6(1): 11-16.
• Bayır B., Gündüz H., Usta T., Şahin E., Özdemir Z., Kayır Ö., Sen Ö., Akşit H., Elmastaş M., Erenler R. Chemical composition of essential oil from Marrubium vulgare L. leaves. Journal of New Results in Science 2014; 6: 44-50.
• Belete T., Boyraz N. Critical review on apple scab (Venturia inaequalis) biology, epidemiology, economic importance, management and defense mechanisms to the causal agent. International Journal of Research Studies in Agricultural Sciences 2018; 4(12): 1-16.
• Bowen JK., Mesarich CH., Bus VG., Beresford RM., Plummer KM., Templeton MD. Venturia inaequalis: the causal agent of apple scab. Molecular plant pathology 2011; 12(2): 105-122.
• Buema G., Ciocîlteu MV., Bejenaru C. Insight into Romanian wild-grown Heracleum sphondylium: Development of a new phytocarrier based on silver nanoparticles with antioxidant, antimicrobial and cytotoxicity potential. Antibiotics 2024; 13(9): 911.
• Deans SG., Sobada KP. Antimicrobial properties of marjoram (Origanum marjorana L.) volatile oil. Flavour and Fragrance Journal 1990; 5: 187-190.
• Demirel Ö., Güneş H., Can C. Sustainable and modern bio-based technologies: new approachs to food safety and security. Environment, Development and Sustainability 2024.
• Dinçel D., Hatipoğlu SD., Gören AC., Topçu G. Anticholinesterase furocoumarins from Heracleum platytaenium, a species endemic to Ida Mountains. Turkish Journal of Chemistry 2013; 37: 675-683.
• Gao Y., Liu Y., Wang ZG., Zhang HL. Chemical constituents of Heracleum dissectum and their cytotoxic activity. Phytochemistry Letters 2014; 10: 276-280.
• Goswami SK., Singh V., Chakdar H., Choudhary P. Harmful effects of fungicides - Current status. International Journal of Agriculture, Environment and Biotechnology 2018; 11: 1011-1019.
• Güneş H., Demir S., Erdinç Ç. How do AMF and Biochar affect pepper growth and nutrient content under biotic and abiotic stress?. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 2025; 28(2): 459-479.
• Işcan G., Özek T., Özek G., Duran A., Başer K. Essential oils of three species of Heracleum: Anticandidal activity. Chemistry of Natural Compounds 2004; 40: 544-547.
• Kılıç CS., Demirci B., Coşkun M., Başer KHC. Chemical composition of Heracleum platytaenium Boiss. (Apiaceae) essential oil from Turkey. Natural Volatiles and Essential Oils 2016; 3: 13-23.
• Koopman TA., Meitz-Hopkins JC., Bester-van der Merwe AE., Tobutt KR., Bester C., Lennox CL. Pathogenicity and virulence of south African isolates of Venturia inaequalis. Eur J Plant Pathol 2022; 164: 45–58.
• Landi L., D’Ortenzio AL., Makau SM., De Miccolis Angelini RM., Romanazzi G. Validation of Monilinia fructicola putative effector genes in different host peach (Prunus persica) cultivars and defense response ınvestigation. Journal of Fungi 2025; 11(1): 39-59.
• Liu M., Liu S., Wu H., Zhang W., Zhang Y., Guo J., Yu H., Chen H. Isolation, antioxidant and antifungal activity of furanocoumarins from the leaves of Heracleum vicinum Boiss. Food Bioscience 2024; 61: 105009.
• Mechergui K., Jaouadi W., Coelho JP., Khouja ML. Effect of harvest year on production, chemical composition and antioxidant activities of essential oil of oregano (Origanum vulgare subsp. glandulosum (Desf.) Ietswaart) growing in North Africa. Industrial Crops and Products 2016; 90: 32-37.
• Pimenov M., Leonov M. The Asian umbelliferae biodiversity database (ASIUM) with particular reference to South-West Asian taxa. Turkish Journal of Botany 2004; 28: 139-145.
• Sajwan K., Sajwan S., Rai N., Negi K., Sharma RK., Khetwal KS. Chemical constituents of Heracleum candicans Wall. Research & Reviews: Journal of Herbal Science 2012; 1(2): 1-9.
• Sayyah M., Moaied S., Kamalinejad M. Anticonvulsant activity of Heracleum persicum seed. Journal of Ethnopharmacology 2005; 98: 209-211.
• Segneanu AE., Vlase G., Vlase T., Bejenaru LE., Mogoşanu GD., Buema G., Herea DD., Ciocîlteu MV., Bejenaru C. Insight into Romanian wild-grown Heracleum sphondylium: Development of a new phytocarrier based on silver nanoparticles with antioxidant, antimicrobial and cytotoxicity potential. Antibiotics 2024; 13(9): 911.
• Soyuçok A. The effects of some essential oils on methicillin-resistant Staphylococcus aureus. Turkish Journal of Agriculture Food Science and Technology 2022; 10(1): 49-53.
• Souri E., Farsam H., Sarkheil P., Ebadi F. Antioxidant activity of some furanocoumarins isolated from Heracleum persicum. Pharmaceutical Biology 2004; 42: 396-399.
• Taniguchi M., Inoue A., Shibano M., Wang NH., Baba K. Five condensed furanocoumarins from the root of Heracleum candicans Wall. Journal of Natural Medicine 2011; 65: 268-274.
• Taniguchi M., Yokota O., Shibano M., Wang NH., Baba K. Four coumarins from Heracleum yunngningense. Chemical and Pharmaceutical Bulletin 2005; 53: 701-704.
• Vasić T., Maširević S., Bulajić A. Morphological, cultural and ecological characterization of Monilinia spp., pathogens of stone fruit in Serbia. Pesticidi i Fitomedicina 2016; 31(1-2): 9-18.
• Wu H., Liu M., Liu S., Yu H., Chen H. Isolation of coumarins with anti-Trichophyton rubrum activity from Heracleum vicinum Boiss. Brazilian Journal of Microbiology 2023; 54(2): 1093-1102.
• Yang LL., Liang YC., Chang CW., Lee WS., Kuo CT., Wang CC., Lee HM., Lin CH. Effects of sphondin, isolated from Heracleum laciniatum, on IL–1β-induced cyclooxygenase-2 expression in human pulmonary epithelial cells. Life Sciences 2002; 72: 199-213.