β-Glukan ve Lactoplantibacillus plantarum GD2’den Elde Edilen L-EPS’nin İn Vitro Yara İyileştirici ve Antibiyofilm Potansiyeli

Öz

Amaç: Bu çalışma, ticari β-glukan ve Lactoplantibacillus plantarum GD2'den elde edilen L-EPS'nin in vitro yara iyileşmesi ve antibiyofilm potansiyelini değerlendirmeyi amaçlamaktadır. Biyopolimerlerin biyouyumluluk, hücre göçü ve biyofilm oluşumunu önleyici etkileri incelenmiştir. Gereç ve Yöntemler: Sitotoksisite, L929 fare fibroblast ve RAW264.7 fare makrofaj hücre hatlarında MTT testi ile analiz edilmiştir. Antibiyofilm aktivitesi mikrobiyal kolonizasyonu önleme yeteneğiyle, yara iyileşme potansiyeli ise çizik testiyle değerlendirilmiştir. Sonuçların istatistiksel anlamlılığı test edilmiştir. Bulgular: Hem β-glukan hem de L-EPS, bireysel ve kombine uygulamalarda biyouyumlu bulunmuştur. Antibiyofilm analizleri, bu biyopolimerlerin mikrobiyal kolonizasyonu önleyerek steril yara ortamını koruyabileceğini ortaya koymuştur. Çizik testi, hücre göçünü teşvik ederek yara kapanmasını hızlandırdıklarını göstermiştir. Kombinasyon, bireysel uygulamalara kıyasla benzer veya daha güçlü bir etki göstermiş, bu da sinerjik etkilere bağlanmıştır. Sonuç: L. plantarum GD2 kaynaklı β-glukan ve L-EPS, yara iyileşmesini hızlandırıcı ve biyofilm oluşumunu önleyici etkileriyle umut verici biyomalzemelerdir. Ancak, biyomedikal uygulamalardaki potansiyellerini doğrulamak için ileri in vivo ve klinik çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler

• Antibiyofilm
• β-glukan
• Ekzopolisakkarit
• Lactoplantibacillus plantarum GD2
• Yara iyileşmesi

In Vitro Wound Healing and Antibiofilm Potential of β-Glucan and L-EPS Obtained from Lactoplantibacillus plantarum GD2

Abstract

Purpose: This study aims to evaluate the in vitro wound healing and antibiofilm potential of commercial β-glucan and L-EPS derived from Lactoplantibacillus plantarum GD2. Biocompatibility, cell migration and biofilm formation inhibitory effects of biopolymers were investigated. Materials and Methods: Cytotoxicity was analyzed using the MTT assay on L929 mouse fibroblast and RAW264.7 mouse macrophage cell lines. Antibiofilm activity was assessed based on microbial colonization prevention, while wound healing potential was evaluated using the scratch test. The statistical significance of the results was tested. Results: Both β-glucan and L-EPS were found to be biocompatible in individual and combined applications. Antibiofilm analyses revealed that these biopolymers can prevent microbial colonization, maintaining a sterile wound environment. Scratch test results demonstrated that they promoted cell migration and accelerated wound closure. The combination showed similar or enhanced effects compared to individual applications, suggesting synergistic properties. Conclusion: β-glucan and L-EPS derived from L. plantarum GD2 are promising biomaterials with wound healing acceleration and biofilm prevention properties. However, further in vivo and clinical studies are required to confirm their biomedical potential.

Keywords

• Antibiofilm
• β-glucan
• Exopolysaccharide
• Lactoplantibacillus plantarum GD2
• Wound healing

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