Ceratophyllum submersum'un Antioksidan, Antikolinesteraz ve Antiproliferatif Aktivitelerinin Değerlendirilmesi

Öz

Bu çalışmada Ceratophyllum submersum etanol özütünün antioksidan, antikolinesteraz ve antiproliferatif aktivitelerinin değerlendirilmesi amaçlanmıştır. Antioksidan aktivite, toplam antioksidan seviyesi (TAS), toplam oksidan seviyesi (TOS) ve oksidatif stres indeksi (OSI) parametreleri ile belirlenmiştir. C. submersum'un TAS değeri 4,271±0,126 mmol/L, TOS değeri 10,505±0,147 µmol/L ve OSI değeri 0,246±0,004 olarak belirlenmiştir. Düşük OSI değeri bitkinin güçlü bir antioksidan savunma sistemine sahip olduğunu göstermektedir. Antikolinesteraz aktivitesi açısından, C. submersum özütü asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) enzimlerine karşı sırasıyla 139,68±2,40 µg/mL ve 171,99±1,81 µg/mL seviyelerinde inhibisyon göstermiştir. Bu değerler standart inhibitör galantamin ile karşılaştırıldığında düşük olsa da özütün potansiyel biyoaktif bileşikler içerdiğini göstermektedir. A549 akciğer kanseri hücre hattında antiproliferatif aktivite değerlendirmesi yapılmıştır. Sonuçlar C. submersum etanol özütünün artan konsantrasyonlarda hücre canlılığını önemli ölçüde azalttığını ve antiproliferatif etki gösterdiğini göstermiştir. Elde edilen bulgular C. submersum'un biyolojik olarak aktif bileşikler içerdiğini ve oksidatif stresle mücadelede etkili bir bitki türü olduğunu göstermektedir. Ancak bu bitkinin biyoyararlanımı, moleküler mekanizmaları ve terapötik potansiyelini daha iyi anlamak için daha fazla çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

• Ceratophyllum submersum
• Antioksidan aktivite
• Antikolinesteraz aktivite
• Antiproliferatif etki
• Oksidatif stres
• Biyoaktif bileşikler

Evaluation of Antioxidant, Anticholinesterase and Antiproliferative Activities of Ceratophyllum submersum

Öz

This study aimed to evaluate the antioxidant, anticholinesterase and antiproliferative activities of Ceratophyllum submersum ethanol extract. Antioxidant activity was determined by the parameters of total antioxidant level (TAS), total oxidant level (TOS) and oxidative stress index (OSI). TAS value of C. submersum was determined as 4,271±0,126 mmol/L, TOS value as 10,505±0,147 µmol/L and OSI value as 0,246±0,004. Low OSI value indicates that the plant has a strong antioxidant defense system. In terms of anticholinesterase activity, C. submersum extract showed inhibition against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes at 139,68±2,40 µg/mL and 171,99±1,81 µg/mL levels, respectively. Although these values are low compared to the standard inhibitor galantamine, they indicate that the extract contains potential bioactive compounds. Antiproliferative activity assessment was performed on A549 lung cancer cell line. The results showed that C. submersum ethanol extract significantly reduced cell viability at increasing concentrations and showed antiproliferative effect. The findings obtained show that C. submersum contains biologically active compounds and is an effective plant species in combating oxidative stress. However, further studies are needed to better understand the bioavailability, molecular mechanisms and therapeutic potential of this plant.

Anahtar Kelimeler

• Ceratophyllum submersum
• Antioxidant activity
• Anticholinesterase activity
• Antiproliferative effect
• Oxidative stress
• Bioactive compounds

Kaynakça

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