İmidakloprid’in Sıçan Kan ve Dalak Dokularında Neden Olduğu Oksidatif Strese Karşı Resveratrol ve Berberin’in Koruyucu Etkileri

Yıl 2026, Cilt: 9 Sayı: 2, 946 - 959, 16.03.2026

Hatice Karaboduk , Çağlar Adıgüzel , Fatma Gökçe Apaydın , Yusuf Kalender

https://doi.org/10.47495/okufbed.1738508

https://izlik.org/JA83HE59DD

Öz

İmidakloprid (IMI) tarımsal mücadelede dünyada en sık kullanılan neonikotinoid insektisitlerden biridir. Aşırı kullanımının sonucu olarak ciddi bir halk sağlığı problemleri ve çevre sorunları ortaya çıkmaktadır. Resveratrol (RES) ve berberin (BBR) antioksidan, antiinflamatuvar ve antikanser gibi özellikleri olan doğal bileşiklerdir. Bu çalışmanın amacı sıçanlarda IMI’nın kan ve dalak dokusu üzerine neden olduğu oksidatif hasara karşı RES ve BBR’nin koruyucu etkisini araştırmaktır. Çalışmada sıçanlar her grupta 6 hayvan olacak şekilde 7 gruba ayrılmıştır. 1. grup: kontrol, 2. grup: RES (20 mg/kg v.a.), 3. grup: BBR (100 mg/kg v.a.), 4. grup: IMI (9 mg/kg v.a.), 5. grup: IMI+RES, 6. grup: IMI+BBR, 7. grup: IMI+RES+BBR. Uygulama maddeleri sıçanlara 28 gün boyunca oral gavaj yolu ile verilmiştir. IMI grubundaki sıçanların kan ve dalak dokularındaki MDA düzeylerinde kontrol grubuna göre anlamlı bir artış (p<0,05), antioksidan enzimler SOD, CAT, GPx ve GST aktivitelerinde ise anlamlı bir azalma (p<0,05) gözlenmiştir. RES ve BBR grupları IMI grubuyla karşılaştırıldığında MDA düzeylerinde anlamlı azalma, antioksidan enzim aktivitelerinde ise anlamlı artış gözlendi (p<0,05). Bu sonuçlar göstermektedir ki IMI’nın kan ve dalak dokusunda oluşturduğu oksidatif hasara karşı, RES ve BBR takviyesinin önemli ölçüde bu toksisiteyi azalttığı tespit edilmiştir.

Anahtar Kelimeler

İmidakloprid , Resveratrol , Berberin , Kan , Dalak , Oksidatif stres

Etik Beyan

Hayvan deneyleriyle ilgili tüm işlemler, Gazi Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu tarafından onaylandı (Etik Kurul No: G.U.ET-23.035).

Teşekkür

Çalışmamızın deney hayvanları kısmını maddi olarak destekleyen Gazi Üniversitesi Bilimsel Araştırma Projeleri birimine teşekkür ederiz.

Protective Effects of Resveratrol and Berberine Against Oxidative Stress Induced by Imidacloprid in Rat Blood and Spleen Tissues

https://izlik.org/JA83HE59DD

Öz

Imidacloprid (IMI) is one of the most commonly used neonicotinoid insecticides in the world for agricultural control. As a result of its overuse, serious public health and environmental problems are emerging. Resveratrol (RES) and berberine (BBR) are natural compounds with antioxidant, anti-inflammatory and anticancer properties. The aim of this study was to investigate the protective effect of RES and BBR against oxidative damage caused by IMI on blood and spleen tissue in rats. In the study, rats were divided into 7 groups with 6 animals in each group. 1st group: control, 2nd group: RES (20 mg/kg v.a.), 3rd group: BBR (100 mg/kg v.a.), 4th group: IMI (9 mg/kg v.a.), 5th group: IMI+RES, 6th group: IMI+BBR, 7th group: IMI+RES+BBR. The administration substances were given to the rats by oral gavage for 28 days. A significant increase in MDA levels in the blood and spleen tissues of rats in the IMI group was found compared to the control group (p<0.05), and a significant decrease in the activities of antioxidant enzymes SOD, CAT, GPx and GST (p<0.05). When the RES and BBR groups were compared with the IMI group, a significant decrease in MDA levels and a significant increase in antioxidant enzyme activities were observed (p<0.05). These results show that RES and BBR supplementation significantly reduced the oxidative damage caused by IMI in blood and spleen tissue.

Anahtar Kelimeler

Imidacloprid , Resveratrol , Berberine , Blood , Spleen , Oxidative stress

Kaynakça

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