Determining the In Situ Ruminal Degradability of Some Nutrients of Wheat Straw Using Some White Rot Fungi Species

Abstract

The Phanerochaete chrysosporium (PC) and Pleurotus eryngii (PE) were observed for their potential to increase the in situ dry matter (DM) and crude protein (CP) degradability of wheat straw. The wheat straw samples were inoculated with the PC and PE fungi (10% weight/volum) and incubated under solid state fermentation conditions at 30 ◦C for 30 days to determine their in-situ degradability and chemical composition. In situ ruminal degradability was determined by using four ruminal cannulated Holstein bulls (approximately 400 kg BW) fed on a diet a concentrate-to-roughage ratio of 45:55 (dry matter (DM) basis). The samples were incubated in nylon bags for 0, 4, 8, 16, 24, 48, 72 and 96 h in the rumen. The results showed that increased in situ ruminal degradation of DM and CP in PC and PE groups in comparison to the control group (P < 0.01). The wheat straw incubated with PC had the greatest in situ DM degradability after incubation for 24 h (P < 0.01). The inoculation of PC and PE increased in situ ruminal crude protein (CP) degradation (P < 0.01) in comparison to the other treatments. As a result, the fungal inoculants and urea treatment increased the degradability of DM and CP in wheat straw.

Keywords

• Wheat straw
• Holstein bulls
• Phanerochaete chrysosporium

Siyah Alacalarda Beyaz Çürükçül Funguslar Kullanılarak Buğday Samanın İn-Situ Parçalanabilirliğinin Belirlenmesi

Öz

Bu araştırmada, Phanerochaete chrysosporium (PC) ve Pleurotus eryngii (PE) mantar türlerinin, buğday samanının in-situ parçalanabilirliği sonucunda kuru madde (KM) ve ham protein (HP) değerleri üzerindeki etkileri incelenmiştir. Buğday samanı örnekleri, PC ve PE fungus türleri ile (%10 ağırlık/hacim) ile inokule edilip, in-situ parçalanabilirlikleri ve kimyasal bileşimleri belirlenmek üzere katı hal fermentasyon koşulları altında 30 °C'de 30 gün inkübe edilmiştir. Kanül takılı 4 baş Siyah Alaca boğa (yaklaşık 400 kg canlı ağırlığa sahip) 45:55 oranında (KM esasına dayalı) konsantre / kaba yem ile beslenmiştir. Yem örnekleri rumende naylon torbalarda 0, 4, 8, 16, 24, 48, 72 ve 96 saat süreyle inkübe edilmiştir. Sonuçlar, PC ve PE'nin inokulasyonunun, kontrol grubuna göre KM ve HP'nin in-situ ruminal parçalanabilirliğini yükselttiğini göstermiştir (P < 0.01). PC ile inkübe edilen buğday samanı, 24 saat inkübasyondan sonra en yüksek in-situ KM parçalanabilirliğine sahip olmuştur (P < 0.01). PC ve PE'nin inokulasyonu, diğer muamelelere göre in-situ rumen ham protein (HP) parçalanabilirliğini (P < 0.01) artırmıştır. Sonuç olarak, fungus inokulayonu ve üre muamelesinin, buğday samanındaki KM ve HP’in parçalanabilirliğini yükselttiği gözlemlenmiştir.

Anahtar Kelimeler

• Buğday samanı,
• Siyah Alaca
• Phanerochaete chrysosporium

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