CRISPR/Cas9 Sistemi ve Gen Düzenleme Teknolojisi

Yıl 2023, Cilt: 6 Sayı: 1, 948 - 957, 10.03.2023

Özlem Gök , Seda Beyaz , Abdullah Aslan

https://doi.org/10.47495/okufbed.1085220

Öz

Covid-19 SARS-CoV-2 (şiddetli akut solunum sendromu-koronavirüs-2), Gen düzenleme teknolojileri üzerine yapılan bu derlemede, CRISPR/Cas9 genom düzenmleme sisteminin özellikleri, uygulama alanları ve geleceği hakkında bilgileri özetlemektedir. Son zamanlarda araştırmacıların amacı, canlı hücrelerin genomunda hedeflenmiş değişiklikler yapmak için uygun maliyetli ve güvenilir yollar geliştirmek olmuştur. CRISPR/Cas9 sisteminin geliştirilmesi sayesinde, moleküler biyoloji, biyomedikal ve tıp gibi birçok alanda ilerleme kaydetmiştir. Dahası hemen hemen her organizmanın ve hücre tipinin DNA'sında hedeflenen kırılmalar yoluyla genom mühendisliği alanında önemli gelişmelere katkı sağlamıştır.

Anahtar Kelimeler

CRISPR, Cas9, Gen ekspresyonu, Genom mühendisliği

CRISPR/Cas9 Systems and Gene Editing Technology

Öz

This review on gene editing technologies summarizes the features, applications and future of the CRISPR/Cas9 genome regulating system. Recently, the aim of searchers has been to improve cost-effective and reliable ways to perform goal modify to the genome of living cells. Thanks to the improvement of the CRISPR/Cas9 system, it has made progress in many fields such as molecular biology, biomedicine and medicine. Moreover, it has contributed to important developments in the field of genome engineering through targeted breaks in the DNA of almost every organism and cell type.

Anahtar Kelimeler

CRISPR, Cas9, Gene expression, Genome engineering

Kaynakça

• Chandrasekaran M., Boopathi T., Paramasivan M. A status-quo review on CRISPR-Cas9 gene editing applications in tomato. International Journal of Biological Macromolecules 2021; 190: 120-129.
• Gupta D., Bhattacharjee O., Mandal D., Sen M.K., Dey D., Dasgupta A., Kazi T.A., Gupta R., Sinharoy S., Acharya K., Chattopadhyay D., Ravichandiran V., Ghosh D. CRISPR-Cas9 system: A new-fangled dawn in gene editing. Life sciences 2019; 232: 116636.
• Gumus N., Kaymaz B.T. CRISPR/Cas9 age in genome editing and leukemia applications. Kafkas Journal of Medical Sciences 2018; 8(3): 232-248.
• Hryhorowicz M., Lipiński D., Zeyland J., Słomski R. CRISPR/Cas9 immune system as a tool for genome engineering. Archivum Immunologiae Et Therapiae Experimentalis 2017; 65(3): 233-240.
• Ilıkkan O.K. Utilization of CRISPR/Cas system of lactic acid bacteria in biotechnology and genetic engineering. Academic Food Journal 2020; 18(3): 303-311.
• Kaur H., Pandey D.K., Goutam U., Kumar V. CRISPR/Cas9-mediated genome editing is revolutionizing the improvement of horticultural crops: Recent advances and future prospects. Scientia Horticulturae 2021; 289: 110476.
• Kozovska Z., Rajcaniova S., Munteanu P., Dzacovska S., Demkova L. CRISPR: History and perspectives to the future. Biomedicine & Pharmacotherapy 2021; 141: 111917.
• Luthra R., Kaur S., Bhandari K. Applications of CRISPR as a potential therapeutic. Life Sciences 2021; 284: 119908.
• Makarova K.S., Koonin E.V. Annotation and classification of CRISPR-Cas systems. CRISPR 2015; 47-75.
• Martinez-Lage M., Torres-Ruiz R., Rodriguez-Perales S. CRISPR/Cas9 technology: Applications and human disease modeling. Progress in Molecular Biology And Translational Science 2017; 152: 23-48.
• Redman M., King A., Watson C., King D. What is CRISPR/Cas9?. Archives of Disease in Childhood-Education and Practice 2016; 101(4): 213-215.
• Savić N., Schwank G. Advances in therapeutic CRISPR/Cas9 genome editing. Translational Research 2016; 168: 15-21.
• Tufan F., Keles E.N. Genome editing technologies and its applications in plants. Haliç University Journal Of Science 2019; 2(1): 113-133.
• Wang S.Y., Du Y.C., Wang D.X., Ma J.Y., Tang A.N., Kong D.M. Signal amplification and output of CRISPR/Cas-based biosensing systems: A review. Analytica Chimica Acta 2021; 338882.
• Xiao-Jie L., Hui-Ying X., Zun-Ping K., Jin-Lian C., Li-Juan J. CRISPR-Cas9: A new and promising player in gene therapy. Journal of Medical Genetics 2015; 52(5): 289-296.
• Xie S., Ji Z., Suo T., Li B., Zhang X. Advancing sensing technology with CRISPR: From the detection of nucleic acids to a broad range of analytes–a review. Analytica Chimica Acta 2021; 338848.
• Xiong X., Chen M., Lim W.A., Zhao D., Qi L.S. CRISPR/Cas9 for human genome engineering and disease research. Annual Review of Genomics And Human Genetics 2016; 17: 131-154.
• Zhang C., Quan R., Wang J. Development and application of CRISPR/Cas9 technologies in genomic editing. Human Molecular Genetics 2018; 27(R2): R79-R88.
• Zhang Y., Nishiyama T., Olson E.N., Bassel-Duby R. CRISPR/Cas correction of muscular dystrophies. Experimental Cell Research 2021; 112844.