The Use of Molasses in Decolorization by Purple Non-Sulfur Bacteria

Yıl 2023, Cilt: 6 Sayı: 2, 1221 - 1229, 05.07.2023

Elif Yürümez Canpolat

Öz

It is well known that waste water discharged from textile industry which contains dye stuff causes environmental pollution. In these aspects, decolorization processes of textile waste water possess much importance. Reactive Black 5 (RB5), which is widely used in textile industry, is a diazo reactive dye. In this study the decolorization ability of purple non-sulphur bacteria strains Rhodopseudomonas palustris, Rhodobacter sphaeroides and Rhodoplanes piscinae in the presence of RB5 and sugar beet molasses as a co-substrate, which is a by-product of sugar production, is investigated. The dye concentration was adjusted as 100 mg/L in culture media. The dye decolorization in media containing molasses as co-substrate was measured spectrophotometrically. The dye degradation was supported by FT-IR analysis. The decolorization percentages in media containing 100 mg/L RB5 were determined as 86.80, 82.37 and 84.06 % by R. piscinae 51ATA, R. palustris DSMZ127 and R. spheroides DSMZ 5864, respectively. It was considered that the dye was used by the bacteria since the aromatic rings of the dye could not be determined by FT-IR analysis on the surface of bacteria. It was also determined from FT-IR analysis that the peaks belonging to the nitrogen in the azo bond were removed as well as the other peaks of the dye resulting in the complete mineralization of the dye by the bacteria

Anahtar Kelimeler

Textil dye, Reactive Black 5, Decolorization, Photosyntetic bacteria, purple non sulphur bacteria

Melasın Mor Kükürtsüz Bakteriler Tarafından Dekolorizasyonda Kullanılması

Öz

Toksik özelliklere sahip tekstil endüstrisinde kullanılan boyar maddelerin deşarj sularının çevre kirliliğine neden olduğu bilinmektedir. Bu bağlamda boyarmadde içeren tekstil endüstrisi atıksularının renk giderim süreçleri ekolojik açıdan büyük önem kazanmaktadır. Reactive Black 5 (RB5) boyası, tekstil endüstrisinde yaygın olarak kullanılan reaktif boyalardan biri olup diazo bir boyadır. Bu çalışmada, mor kükürtsüz fotosentetik bakteriler olan Rhodopseudomonas palustris, Rhodobacter sphaeroides ve Rhodoplanes piscinae suşlarının RB5 boyasını dekolorizasyon yeteneği, şeker atığı olan melas yardımcı substratı varlığında araştırılmıştır. Boya konsantrasyonu 100 mg/L olacak şekilde besiyerlerine ilave edilmiştir. Yardımcı substratın kullanıldığı ortamda boyanın dekolorizasyonu, spektrofotometrik olarak takip edilmiştir. FTIR analizleri ile boya gideriminin tespiti desteklenmiştir. 100 mg/L RB5 boyasını içeren besiyerinde R. piscinae 51ATA suşunun %86,80 oranında, R. palustris DSMZ127 suşunun %82,37 oranında ve R. sphaeroides DSMZ 5864 suşunun ise %84,06 oranında dekolorizasyon gerçekleştirdiği tespit edilmiştir. FT-IR analizinde, bakteri yüzeyinde boyanın aromatik halkalarının görülmemesi boyanın bakteri tarafından kullanıldığını düşündürmüştür. Boyanın FT-IR spektrumlarından, diğer pikler gibi azo bağlarındaki azotun da dahil olduğu azo piklerinin uzaklaştığı, boyanın bakteri tarafından tamamen mineralize edildiği tespit edilmiştir.

Anahtar Kelimeler

Tekstil boyası, Reactive black 5, Dekolorizasyon, Fotosentetik bakteriler, mor kükürtsüz bakteriler

Kaynakça

• Referans 1 Bhatia D., Sharma NR., Singh J., Kanwar, RS. Biological methods for textile dye removal from wastewater: A review. Critical Reviews in Environmental Science and Technology 2017; 47(19): 1836-1876.
• Referans 2 Bilal M., Rasheed T., Iqbal H., Hu H., Wang W., Zhang X. Toxicological assessment and UV/TiO2-based induced degradation profile of reactive black 5 dye. Environmental Management 2018; 61(1): 171-180.
• Referans 3 Chen G., Huang MH., Chen L., Chen DH. A batch decolorization and kinetic study of Reactive Black 5 by a bacterial strain Enterobacter sp. GY-1. International Biodeterioration and Biodegradation 2011; 65: 790-796.
• Referans 4 Çelik L., Öztürk A. Abdullah MI. Biodegradation of reactive red 195 azo dye by the bacterium Rhodopseudomonas palustris 51ATA. Afr. J. Microbiol. Res. 2012; 6(1): 120-126.
• Referans 5 Droguett T., Mora-Gomez J., García-Gabaldon M., Ortega E., Mestre S., Cifuentes G., Perez-Herranz V. Electrochemical Degradation of Reactive Black 5 using twodifferent reactor configuration. Scientific Reports 2020; 10(1): 1-11.
• Referans 6 El Bouraie M. El Din WS. Biodegradation of Reactive Black 5 by Aeromonas hydrophila strain isolated from dye-contaminated textile wastewater. Sustainable Environment Research, 2016; 26(5): 209-216.
• Referans 7 Eslami H., Shariatifar A., Rafiee E., Shiranian M., Salehi F., Hosseini SS. Ebrahimi AA. Decolorization and biodegradation of reactive Red 198 Azo dye by a new Enterococcus faecalis–Klebsiella variicola bacterial consortium isolated from textile wastewater sludge. World Journal of Microbiology and Biotechnology 2019; 35(3): 1-10.
• Referans 8 Guo JZ., Li B., Liu L., Lv K. Removal of methylene blue from aqueous solutions by chemically modified bamboo. Chemosphere 2014; 111: 225-231.
• Referans 9 Jadhav JP., Kalyani DC., Telke AA., Phugare SS., Govindwar SP. Evaluation of the efficacy of a bacterial consortium for the removal of color, reduction of heavy metals, and toxicity from textile dye effluent. Bioresour Technol. 2010; 101: 165–173.
• Referans 10 Jamee R., Siddique R. Biodegradation of synthetic dyes of textile effluent by microorganisms: an environmentally and economically sustainable approach. Eur. J Microbiol. Immunol. 2019; 9: 114–118.
• Referans 11 Lops C., Ancona A., Di Cesare K., Dumontel B., Garino N., Canavese G., Cauda V. Sonophotocatalytic degradation mechanisms of Rhodamine B dye via radicals generation by micro-and nano-particles of ZnO. Applied Catalysis B: Environmental 2019; 243: 629-640.
• Referans 12 Mourid E., Lakraimi M., Khattabi E., El Benaziz L., Berraho M. Removal of remazol brilliant blue R from aqueous solution by adsorption using a calcined layered double hydroxide [Zn2-Al-CO3]. J. Mater. Environ. Sci. 2017; 8: 921–930.
• Referans 13 Neoh CH., Lam CY., Lim CK., Yahya A., Bay HH., Ibrahim Z., Noor ZZ. Biodecolorization of recalcitrant dye as the sole sourceof nutrition using Curvularia clavata NZ2 and decolorization ability of its crude enzymes. Environmental Science and Pollution Research 2015; 22(15): 11669–11678.
• Referans 14 Rai HS., Bhattacharya MS., Singh J., Bansal TK.,Vats P., Banerjee UC. Removal of dyes from the effluent of textile and dyestuff manufacturing industry: a review of emergin techniques with reference to biological treatment. Crit. Rev. Environ. Sci. Technol. 2005; 35: 219–238.
• Referans 15 Samir Ali S., Al-Tohamy R., Sun J., Wu J., Huizi L. Screening and construction of a novel microbial consortium SSA-6 enriched from the gut symbionts of wood-feeding termite, Coptotermes formosanus and its biomass-based biorefineries. Fuel 2019; 236: 1128-1145.
• Referans 16 Seyedi ZS., Zahraei Z., Jookar Kashi F. Decolorization of reactive black 5 and reactive red 152 Azo dyes by new haloalkaliphilic bacteria isolated from the textile wastewater. Current Microbiology 2020; 77(9): 2084-2092.
• Referans 17 Tan NCG., Prenafeta-Boldu FX., Opsteeg JL., Lettinga G., Field JA. Biodegradation of azo dyes in cocultures of anaerobic granular sludge with aerobic aromatic amine degrading enrichment cultures. Applied Microbiology and Biotechnology 1999; 51(6): 865-871.
• Referans 18 Wenning M., Breitenwieser F., Konrad R., Huber I., Busch U., Scherer S. Identification and differentiation of food-related bacteria: A comparison of FTIR spectroscopy and MALDI-TOF mass spectrometry. Journal of Microbiological Methods 2014; 103: 44–52.