Gibberellic Acid is Active Only in Orchid Cross-Pollination

Yıl 2025, Cilt: 8 Sayı: 2, 533 - 542, 12.03.2025

Mehmet Aybeke

https://doi.org/10.47495/okufbed.1452135

Öz

The aim of this study is to determine the endogenous hormone activities of Gibberellic acid (GA) in compatible (Intraspecific= ISP) and incompatible pollination (Intergeneric= IGP). It was designed to be Himantoglossum robertianum in ISP experiments and Himantoglossum robertianum and Orchis italica in IGP experiments. For this reason, some polynariums taken from O. italica have been applied with needles to the flower stigmas of H. robertianum. Ovaries with stigma have been taken from both types of pollination for 10 days, and quantitative hormone analyses have been performed by LC-MS/MS. As a result, GA was not found in the ISP experiments. Likewise, it did not appear at all in the tests between the 1st and 6th days of IGP; it was found only on the 7th, 8th, 9th, and 10th days of IGP and at increasingly higher values. For the first time, endogenous ovary-stigma GA amounts in the post-pollination process of orchid IGP were determined in this study, and its importance was discussed. According to statistical analysis, there is a significant difference between almost all values. It has been understood that additional histological and embryological studies are needed to understand the reason for the very high activity, especially on days 9 and 10.

Anahtar Kelimeler

Himantoglossum, hormone, orchide, post polination phenomena, flower

Gibberellik Asit Yalnızca Orkidelerin Çapraz Tozlaşmasında Aktiftir

Öz

Bu çalışmanın amacı Gibberellik asitin (GA) uyumlu (Intraspesifik=ISP) ve uyumsuz tozlaşmada (Intergeneric=IGP) endojen hormon aktivitelerini belirlemektir. ISP deneylerinde Himantoglossum robertianum ve IGP deneylerinde Himantoglossum robertianum ve Orchis italica olacak şekilde tasarlandı. Bu nedenle O. italica'dan alınan bazı polinariumlar H. robertianum'un çiçek stigmalarına iğnelerle uygulanmıştır. Her iki tozlaşma türünden de stigmalı yumurtalıklar 10 gün süreyle alınmış ve LC-MS/MS ile kantitatif hormon analizleri yapılmıştır. Sonuç olarak ISP deneylerinde GA bulunamadı. Aynı şekilde IGP'nin 1. ve 6. günleri arasında yapılan testlerde de hiç görünmedi; IGP'nin sadece 7, 8, 9 ve 10. günlerinde ve giderek daha yüksek değerlerde bulundu. İlk kez bu çalışmada orkide IGP'nin tozlaşma sonrası süreçteki endojen yumurtalık-stigma GA miktarları belirlenmiş ve önemi tartışılmıştır. İstatistiksel analizlere göre hemen hemen tüm değerler arasında anlamlı bir fark vardır. Özellikle 9. ve 10. günlerde görülen çok yüksek aktivitenin nedeninin anlaşılabilmesi için ilave histolojik ve embriyolojik çalışmalara ihtiyaç duyulduğu anlaşılmıştır.

Anahtar Kelimeler

Himantoglossum, hormon, orkide, tozlaşma sonrası olaylar, çiçek

Teşekkür

The author would like to thank Prof. Dr. Oğuzhan Doğanlar for his help in statistical tests.

Kaynakça

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