Characterization of Pseudomonas species isolated from agricultural areas applied with sulphonylurea group herbicides in Tekirdağ province

Öz

The role of bacteria in biological degradation and removal of toxic properties of pollutants has been set out in various studies. Like many toxic chemicals, biological degradation of sulphonylurea group herbicides has been studied with increasing interest in recent years. In this study, 134 isolates of Pseudomonas genus were isolated and purified from intense-herbicide applied agricultural areas, where wheat and corn were cultivated in rotation, in Tekirdağ province. Among these, 45 isolates which survived at high herbicide concentration [500 mgL-1 (w/v)] were selected. The selected isolates showed the ability of nitrogen fixation (42 isolates) and phosphate solubilization (31 isolates), nitrate reduction (37 isolates), lipolytic (45 isolates) and proteolytic (7 isolates) activity. In addition, genotypic characterization of the isolates was performed by classical biochemical and physiological tests. Five of these isolates were identified at the species level by comparison with the data in the NCBI database by molecular biological methods (16S rDNA sequence analysis). The identification results were evaluated according to the first three matches with the highest identity rate (100-99%) and isolates were identified as Pseudomonas cremicolorata, Pseudomonas parafulva, Pseudomonas putida (isolate 13); Pseudomonas baetica, Pseudomonas koreensis and Pseudomonas helmanticensis (isolate 18); Pseudomonas plecoglossicida, Pseudomonas cremicolorata; Pseudomonas parafulva (isolate 28); Pseudomonas reidholzensis and Pseudomonas putida (isolates 38 and 42) .

Anahtar Kelimeler

• Biochemical and molecular characterization
• Degradation
• Pseudomonas
• Sulphonylurea
• 16SrDNA

Sülfonilüre Grubu Herbisit Kullanılan Tekirdağ Tarım Alanlarından İzole Edilen Pseudomonas Türlerinin Karakterizasyonu

Öz

Toksik kirleticilerin biyolojik parçalanma ve toksik özelliklerinin gideriminde bakterilerin rolü çeşitli araştırmalarda ortaya konmuştur. Birçok toksik kimyasal gibi sülfonilüre grubu herbisitlerin de biyolojik olarak yıkımı konusu son yıllarda artan bir ilgiyle çalışılmaktadır. Bu çalışmada herbisitlerin yoğun olarak kullanıldığı buğday ve mısırın rotasyonlu ekildiği Tekirdağ tarım alanlarından Pseudomonas cinsinin temel özellikleri ile uyumlu 134 izolat saflaştırılmıştır. Bunların içinden yüksek herbisit konsantrasyonunda [500 mgL-1 (w/v)] hayatta kalan 45 izolat seçilmiştir. Seçilen izolatlar azotu fikse etme (42 izolat) ve fosfatı çözebilme (31 izolat), nitratı indirgeme (37 izolat), lipolitik (45 izolat) ve proteolitik (7 izolat) aktivite göstermiştir. Bunun yanında izolatların klasik biyokimyasal ve fizyolojik testler ile cins seviyesinde karakterizasyonu yapılmıştır. Bu izolatlardan 5 tanesi ise moleküler biyolojik yöntemler (16S rDNA dizi analizi) ile NCBI veri bankasındaki veriler ile karşılaştırılarak tür seviyesinde de tanımlanmıştır. Tanımlama sonuçları en yüksek benzerliğe (%100-99) sahip ilk üç eşleşmeye göre değerlendirilmiş ve izolatlar Pseudomonas cremicolorata, Pseudomonas parafulva; Pseudomonas putida (izolat 13); Pseudomonas baetica, Pseudomonas koreensis ve Pseudomonas helmanticensis (izolat 18); Pseudomonas plecoglossicida, Pseudomonas cremicolorata; Pseudomonas parafulva (izolat 28); Pseudomonas reidholzensis, Pseudomonas putida (izolat 38 ve 42) olarak tanımlanmıştır.nas cremicolorata; Pseudomonas parafulva (izolat 28); Pseudomonas reidholzensis, Pseudomonas putida (izolat 38 ve 42) olarak tanımlanmıştır.

Anahtar Kelimeler

• Biyokimyasal ve moleküler karakterizasyon
• degradasyon
• Pseudomonas
• sülfonilüre
• 16S rDNA

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