THE EFFECT OF GENERATION AND PARENTS ON ENDOSPERM PROTEIN RATIO AND CHANGE OF PROTEIN FRACTIONS IN MAIZE

Yıl 2013, Cilt: 28 Sayı: 3, 150 - 156, 05.10.2013

Cem Egesel , Fatih Kahrıman , Nur Çorbacıoğlu

https://doi.org/10.7161/anajas.2013.28.3.150

Cited By: 1

Öz

Among the quality traits of maize grain, protein ratio and protein quality are two main components. The objective of the study was to investigate changes of endosperm protein ratio and protein fractions in successive generations of different hybrid combinations having a high lysine mutant parent, opaque2 (o2). The study was carried out using seed samples generated at 2010 and 2011 growing seasons in Dardanos Research and Experiment Center of Çanakkale Onsekiz Mart University. To produce the plant material, an o2 line was pollinated with two different inbreds. The seeds of F0:1 and F1:2 generations from these hybrids, as well as selfed seeds from the parents, were used to detect protein ratio and protein fractions. The results showed that there were significant differences between parent lines and F1:2 seeds of hybrid combinations for endosperm protein ratios. Endosperm protein ratios ranged between 5.54% and 8.93%. There was a significant male parent effect on albumin + globulin and zein fractions as well as total protein ratio. Albumin + globulin fractions ranged between 3.46-8.71 mg/g. Lower values were detected for zein and total protein ratios in F1:2 seeds as compared to the seeds of F0:1 generation. In terms of zein fractions the range was between 16.9 and 61.0 mg/g. 

Anahtar Kelimeler

Albumin, Globulin, Protein, Zea mays

MISIRDA ENDOSPERM PROTEİN ORANI VE PROTEİN FRAKSİYONLARININ DEĞİŞİMİNDE EBEVEYN VE GENERASYONLARIN ETKİSİ

Öz

Mısırda tane kalitesinin belirleyici unsurları arasında, protein oranı ve protein kalitesi önemli yer tutmaktadır. Bu çalışmanın amacı, yüksek lisin içeriğine sahip opaque-2 (o2) mutant hattı ile oluşturulan farklı melez kombinasyonların endosperm protein oranının ve portein fraksiyonlarının farklı generasyonlarda değişiminin incelenmesidir. Çalışma Çanakkale Onsekiz Mart Üniversitesi, Ziraat Fakültesi Dardanos Araştırma ve Uygulama Birimi’nde 2010 ve 2011 yetiştirme sezonlarında oluşturulan tohumluk setleri kullanılarak yürütülmüştür. Araştırmada bir o2 hattı iki farklı baba hat ile melezlenmiş ve bu melezlerin F0:1 ve F 1:2 tohumlukları ile ebeveyn hatların kendilenmiş örneklerinde protein oranı ve protein fraksiyonlarını belirlemek için analizler yapılmıştır. Araştırma bulgularına göre endosperm protein oranı bakımından ebeveyn hatlar ve hibrit kombinasyonların F1:2 generasyonundaki tohumlukları arasında önemli ölçüde farklılık olduğu görülmüştür. Genotiplerin endosperm protein oranı % 5.54-8.93 arasında değişim göstermiştir. Baba hatların etkisinin albümin+globülin ve zein proteinlerinin içeriği kadar, toplam protein içeriğine de etkili olduğu görülmüştür. Albümin+globülin fraksiyonları 3.46-8.71 mg/g arasında değişim göstermiştir. Ayrıca zein ve toplam protein içeriğinin, F1:2 generasyonunda F0:1 generasyonuna göre daha düşük olduğu tespit edilmiş, zein proteinleri ise 16.9-61.0 mg/g arasında değişmiştir.

Anahtar Kelimeler

Albümin, Globülin, Protein, Zea mays

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