FACTORS AFFECTING MICROTUBERIZATION OF POTATO (SOLANUM TUBEROSUM L.) ON IN VITRO CONDITIONS

Yıl 2014, Cilt: 29 Sayı: 2, 154 - 165, 11.06.2014

Ahmet Kumlay , Neşet Arslan , Canan Kaya

https://doi.org/10.7161/anajas.2014.29.2.154-165

Cited By: 1

Öz

Micropropagation and microtuberization of potato (Solanum tuberosum L.) by in vitro culture was commonly used for the production of disease-free plantlets, germplasm conservation and exchange, and seed tuber production. Research on microtuberization in potato has mainly focused on the use of plant growth regulators however there is a significant variation in the results of these studies. Therefore, it is important to understand factors affecting microtuberization. The purpose of this review is to examine the effects of cultivar, explant source and types, inoculation density, solidifying support material, photoperiod, temperature, composition of carbohydrates, content of nitrogen and potassium in nutrient medium in addition to plant growth regulators in microtuberization of potato plantlets cultivated in vitro.

Anahtar Kelimeler

Potato, tissue culture, in vitro, microtuber, hormones

PATATES (SOLANUM TUBEROSUM L.)’TE IN VITRO ŞARTLARDA MİKROYUMRU ELDE EDİLMESİNİ ETKİLEYEN FAKTÖRLER

Öz

Hastalıktan ari fide elde edilmesi, germplazm muhafazası ve değişimi, ve tohumluk yumru elde edilmesi gibi amaçlarla patates (Solanum tuberosum L.) bitkisinin in vitro şartlarda mikroçoğaltımı ve mikroyumru (MY) elde edilmesi yaygın olarak kullanılmaktadır. Patateste MY araştırmaları temelde bitki büyüme düzenleyicileri üzerine yoğunlaşmış, ancak bu çalışmaların sonuçlarında önemli farklılıklar olduğu belirlenmiştir. Bu derlemenin amacı; in vitro şartlarda kültüre alınmış patates bitkiciklerinin MY meydana getirme özellikleri üzerine, bitki büyüme düzenleyicileri yanında çeşidin, eksplant kaynağı ve tipinin, inokülasyon yoğunluğunun, katılaştırıcı destek maddesinin, fotoperiyotun, sıcaklığın, karbonhidratın, kültür ortamındaki azot ve potasyum içeriğinin etkilerini araştırmaktır.

Anahtar Kelimeler

Patates, doku kültürü, in vitro, mikroyumru, hormonlar

Kaynakça

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