In vitro effects of some forage industry chemicals on hyaluronidase

Yıl 2016, Cilt: 31 Sayı: 3, 399 - 407, 31.10.2016

Mustafa Oğuzhan Kaya , Emrah Yerlikaya , Mehmet Arif Özyazıcı , Kıvanç İrak

https://doi.org/10.7161/omuanajas.269994

Öz

This study investigates in vitro effects of some chemicals, used as a spreading factor on therapeutic and various medical fields and applied frequently in forage and fertilizer industry on bovine testicular hyaluronidase (BTH) which is substantially effective in glucotechnological applications and delivery of artificial insemination. BTH was purified through ammonium sulfate precipitation method and affinity gel (Sepharose-4B-L- tyrosine -m-anisidine). The inhibiton effects of Fe2SO4, C2H4N4, K2SiF6, CH2N2, CH4N2O and indole-butyric acid were determined on purified BTH. IC50 values of these chemicals were found as 7.097, 5.775, 6.854, 5.317, 4.617, 4.265 ×10-2 mM respectively and at the end of research, indole-butyric acid (IBA) was found as the strongest inhibitory effect compound by the value of IC50 with 4.265×10-2 mM.

Anahtar Kelimeler

Fertilizer, hyaluronidase; inhibition; artificial insemination; forage

Bazı yem ve gübre sanayi kimyasallarının hyalüronidaz üzerine in vitro etkileri

Öz

Bu çalışmada, terapötik ve çeşitli tıbbi alanlarda yayılma faktörü olarak kullanılan, glikoteknolojik uygulamalar ve suni tohumlamada önemli derecede etkili sığır testis hyalüronidazı (BTH: bovine testicular hyaluronidase) üzerine yem ve gübre sanayisinde sıkça kullanılan bazı kimyasalların in vitro etkileri araştırılmıştır. BTH, amonyum sülfat çöktürmesi yöntemi ve afinite jeli (Sepharose-4B-L-tirozin-m-anisidin) kullanılarak saflaştırılmıştır. Saflaştırılan BTH enzimi üzerine Fe2SO4, C2H4N4, K2SiF6, CH2N2, CH4N2O ve İndol-Bütirik Asitin inhibisyon etkileri belirlenmiştir. Bu kimyasallar için IC50 değerleri sırasıyla 7.097, 5.775, 6.854, 5.317, 4.617, 4.265 ×10-2 mM olarak bulunmuş ve araştırma sonucunda, IC50 değeri 4.265×10-2 mM ile indol-bütirik asit (IBA) en güçlü inhibisyon etkisi gösteren yapı olduğu tespit edilmiştir.

Anahtar Kelimeler

Gübre, hyalüronidaz; inhibisyon; suni tohumlama; yem

Kaynakça

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