In vitro effects of some forage industry chemicals on hyaluronidase

Yıl 2016, Cilt: 31 Sayı: 3, 399 - 407, 31.10.2016

Mustafa Oğuzhan Kaya Emrah Yerlikaya Mehmet Arif Özyazıcı Kıvanç İrak

https://doi.org/10.7161/omuanajas.269994

Öz

This study investigates in vitro effects of some chemicals, used as a spreading factor on therapeutic and various medical fields and applied frequently in forage and fertilizer industry on bovine testicular hyaluronidase (BTH) which is substantially effective in glucotechnological applications and delivery of artificial insemination. BTH was purified through ammonium sulfate precipitation method and affinity gel (Sepharose-4B-L- tyrosine -m-anisidine). The inhibiton effects of Fe2SO4, C2H4N4, K2SiF6, CH2N2, CH4N2O and indole-butyric acid were determined on purified BTH. IC50 values of these chemicals were found as 7.097, 5.775, 6.854, 5.317, 4.617, 4.265 ×10-2 mM respectively and at the end of research, indole-butyric acid (IBA) was found as the strongest inhibitory effect compound by the value of IC50 with 4.265×10-2 mM.

Anahtar Kelimeler

Fertilizer, hyaluronidase; inhibition; artificial insemination; forage

Bazı yem ve gübre sanayi kimyasallarının hyalüronidaz üzerine in vitro etkileri

Öz

Bu çalışmada, terapötik ve çeşitli tıbbi alanlarda yayılma faktörü olarak kullanılan, glikoteknolojik uygulamalar ve suni tohumlamada önemli derecede etkili sığır testis hyalüronidazı (BTH: bovine testicular hyaluronidase) üzerine yem ve gübre sanayisinde sıkça kullanılan bazı kimyasalların in vitro etkileri araştırılmıştır. BTH, amonyum sülfat çöktürmesi yöntemi ve afinite jeli (Sepharose-4B-L-tirozin-m-anisidin) kullanılarak saflaştırılmıştır. Saflaştırılan BTH enzimi üzerine Fe2SO4, C2H4N4, K2SiF6, CH2N2, CH4N2O ve İndol-Bütirik Asitin inhibisyon etkileri belirlenmiştir. Bu kimyasallar için IC50 değerleri sırasıyla 7.097, 5.775, 6.854, 5.317, 4.617, 4.265 ×10-2 mM olarak bulunmuş ve araştırma sonucunda, IC50 değeri 4.265×10-2 mM ile indol-bütirik asit (IBA) en güçlü inhibisyon etkisi gösteren yapı olduğu tespit edilmiştir.

Anahtar Kelimeler

Gübre, hyalüronidaz; inhibisyon; suni tohumlama; yem

Kaynakça

• Alexander, N.J., 1977. Surface structure of spermatozoa frozen for artificial insemination. Andrologia, 9(2): 155-161.
• Baumgartner, G., Moritz, A., 1988. Hyaluronidase. Springer-Verlag Wien New York, 1-8.
• Boling, S.D., Edwards, H.M., Emmert, J.L., Biehl, R.R., Baker, D.H., 1998. Bioavailability of Iron in Cottonseed Meal, Ferric Sulfate, and Two Ferrous Sulfate By-Products of the Galvanizing Industry. Poultry Science, 77: 1388-1392.
• Botzki, A., Rigden, D.J., Braun S., Nukui, M., Salmen, S., Hoechstetter, J., Bernhardt, G., Dove, S., Jedrzejas, M.J., Buschauer, A., 2004. L-Ascorbic Acid 6-Hexadecanoate, a Potent Hyaluronidase Inhibitor. The Journal of Biological Chemistry, 279(44): 45990-45997.
• Cherr, G.N., Yudin, A.I., Overstreet, J.W., 2001. The dual functions of GPI-anchored PH-20: hyaluronidase and intracellular signaling. Matrix Biol, 20(8): 515-25.
• Csoka, T.B., Frost, G.I., Stern, R., 1997. Hyaluronidases in tissue invasion. Invasion & Metastasis, 17(6): 297-311.
• Dai, Y., Di, H.J., Cameron, K.C., He, J.Z., 2013. Effects of nitrogen application rate and a nitrification inhibitor dicyandiamide on methanotroph abundance and methane uptake in a grazed pasture soil. Environmental Science and Pollution Research International, 20(12): 8680-8689.
• Fallis, A., 2013. Structural and functıional studies on group a streptococcal bacteriophage hyaluronidase. Journal of Chemical Information and Modeling, 53(9): 1689-1699.
• Frost, G.I., Csóka, T., Stern, R. 1996. The hyaluronidases: A chemical, biological and clinical overview. Trends in Glycoscience and Glycotechnology, 8(44): 419-434.
• Gladys, L., Hobby, P.H.D., Martin H., Dawson M.D., Karl Meyer, M.D., Eleanor, C., 1940. The relationship between spreding factor and Hyaluronidase, 9: 109-123.
• Grenier, D., Michaud, J., 1993. Evidence for the absence of hyaluronidase activity in porphyromonas gingivalis. Journal of Clinical Microbiology, 31(7): 1913-1915.
• Guo, X., Shi, Y., Sheng, J., Wang, F., 2014. A novel hyaluronidase produced by Bacillus sp. A50. PLoS ONE, 9(4): 1-8.
• Guthrie, R.D., 1969. Macromolecules. Carbohydrate Chemistry, 2: 215-216.
• Hertel, W., Peschel, G., Ozegowski, J.H., Müller, P.J., 2006. Inhibitory Effects of Triterpenes and Flavonoids on the Enzymatic Activity of Hyaluronic Acid-Splitting Enzymes. Arch. Pharm. Chem. Life Sci., 339: 313–318.
• Hoechstetter, J., 2005. Characterisation of bovine testicular hyaluronidase and a hyaluronate lyase from Streptococcus agalactiae. Tez, 1-239.
• Jackson, J., New, R., Fellow, S., 1927. Metabolism of Tryptophane.The Journal Of Biological Chemistry, 84: 1-21.
• Jin, M., Fujiwara, E., Kakiuchi, Y., Okabe, M., Satouh, Y., Baba, S.A., Chiba, K., Hirohashi, N., 2011. Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization. Proceedings of the National Academy of Sciences of the United States of America, 108(12): 4892-4896.
• Kakizaki, I., Ibori, N., Kojima, K., Yamaguchi, M., Endo, M., 2010. Mechanism for the hydrolysis of hyaluronan oligosaccharides by bovine testicular hyaluronidase. FEBS Journal, 277(7): 1776-1786.
• Kaya, M.O., 2013. Yeni bir metodla hyalüronidaz enziminin saflaştırılması ve bazı bileşiklerin bu enzim üzerine etkilerinin araştırılması.Tez; 1-123.
• Kaya, M.O., Arslan, O., Güler, O.O. 2014. A new affinity method for purification of bovine testicular hyaluronidase enzyme and an investigation of the effects of some compounds on this enzyme. Journal of Enzyme Inhibition and Medicinal Chemistry, 6366: 1-4.
• Kazemi-Bonchenari, M., Salem, A.Z.M., Ghasemi, E., 2016. Effect of urea supplementation in diet based on barley grain or corn silage on performance, digestion, rumen fermentation and microbial protein synthesis in Holstein bull calves. Indian Journal of Animal Sciences, 86(3): 313-317.
• Kleigrewe, K., Niehaus, E.M., Wiemann, P., Tudzynski, B., Humpf, H.U., 2012. New approach via gene knockout and single-step chemical reaction for the synthesis of isotopically labeled fusarin c as an internal standard for the analysis of this fusarium mycotoxin in food and feed samples. Journal of Agricultural and Food Chemistry, 60(34): 8350-8355.
• Kreil, G., 1995. Hyaluronidases-a group of neglected enzymes. Protein Science : A Publication of the Protein Society, 4(9): 1666-1669.
• Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259): 680-685.
• Luo, J., Ledgard, S., Wise, B., Welten, B., Lindsey, S., Judge, A., Sprosen, M., 2015. Effect of dicyandiamide (DCD) delivery method, application rate, and season on pasture urine patch nitrous oxide emissions. Biology and Fertility of Soils, 51(4): 453-464.
• Mahmood, T., Ali, R., Latif, Z., Ishaque, W., 2011. Dicyandiamide increases the fertilizer N loss from an alkaline calcareous soil treated with 15N-labelled urea under warm climate and under different crops. Biology and Fertility of Soils, 47(6): 619-631.
• Meyer, M.F., Kreil, G., Aschauer, H., 1997. The soluble hyaluronidase from bull testes is a fragment of the membrane-bound PH-20 enzyme. FEBS Letters, 413(2): 385-388.
• Pant, B., Kohda, H., Namera, A., 1996. Clonal propagation of cnidium officinale by shoot tip culture. Planta Medica, 62(3): 281-283.
• Rushing, L.G., Hansen, E.B.J., Thompson, H.C.J., 1985. Analysis of D-penicillamine by gas chromatography utilizing nitrogen--phosphorus detection. Journal of Chromatography, 337(1): 37-46.
• Saadullah, M., Haque, M., Dolberg, F., 1981. Effectiveness of Ammonification through Urea in Improving the Feeding Value of Rice Straw in Ruminants. Trop. Anim. Prod. 6(1978): 30-36.
• Sluis, J.J., 1987. Investigations Into The Outer Surface of pathogenic treponema pallidum. Rotterdam Üniversitesi. Tez. 1-140.
• Suzuki, A., Toyoda, H., Toida, T., Imanari, T., 2001. Preparation and inhibitory activity on hyaluronidase of fully O-sulfated hyaluro-oligosaccharides. Glycobiology, 11(1): 57-64.
• Tanyildizi, S., Bozkurt, T., 2003. The effects of lincomycin-spectinomycin and sulfamethoxazole-trimethoprim on hyaluronidase activities and sperm characteristics of rams. The Journal of Veterinary Medical Science / The Japanese Society of Veterinary Science, 65(7): 775-780.
• Tong, X.H., Wu, L.M., Jin, R.T., Luo, L.H., Luan, H.B., Liu, Y.S., 2012. Fertilization rates are improved after IVF if the corona radiata is left intact in vitrified-warmed human oocytes. Human Reproduction, 27(11): 3208-3214.
• Wang, H., Li, R., Fan, C., Feng, J., Jiang, S., Han, Z., 2015. Removal of fluoride from the acid digestion liquor in production process of nitrophosphate fertilizer. Journal of Fluorine Chemistry, 180: 122-129.