Pestisit klorprifosun neden olduğu testis doku hasarı üzerine kurkuminin antioksidan etkisinin ışık mikroskobik olarak incelenmesi

Yıl 2017, Cilt: 32 Sayı: 1, 139 - 146, 14.02.2017

Banu Eren , Sevcan Mercan , Nazan Dinç

https://doi.org/10.7161/omuanajas.289406

Cited By: 1

Öz

Bu çalışmada zirai mücadelede yaygın olarak kullanılan bir organofosfat pestisit olan klorprifosun (CPF) memeli testis dokusu üzerinde oluşturduğu hasar ve bu hasarın azaltılması amacıyla kullanılan antioksidan kurkuminin etkisi araştırılmıştır. Bu amaçla, 48 adet 90 günlük erkek rat (Wistar albino) 15 gün boyunca madde uygulanan ve 30 gün boyunca madde uygulanan grup olmak üzere öncelikle iki gruba ayrılmıştır. Bu gruplar da kendi içerisinde bir kontrol grubu ile sırasıyla sadece CPF, sadece kurkumin ve CPF+kurkumin verilen 3 muamele grubu olacak şekilde toplam dört grup oluşturulmuştur. 15. ve 30. günlerde gruplardaki 6’şar hayvandan testis dokuları alınmıştır. Hayvanlardan çıkarılan testis dokuları %10’luk tamponlanmış nötral formalin içerisine alınmış, histolojik takip işlemlerinden sonra ışık mikroskobunda değerlendirilmiştir. Yapılan değerlendirmede CPF grupları kontrol grubuyla karşılaştırıldığında seminifer tübüllerde dejenerasyon, spermatogenik hücrelerde azalma, seminifer tübüller arasındaki bölgede bağ dokusunda ödem, nekrozis gibi histopatolojik değişiklikler belirlenmiştir. CPF ve kurkuminin birlikte verildiği gruplarda CPF gruplarına göre daha az histopatolojik değişiklik gözlenmiştir. Kontrol ile CPF ve CPF+KUR grupları arasında ve CPF ile CPF+KUR grupları arasındaki fark istatistiksel olarak anlamlı bulunmuştur (p<0.05). Bulgular incelenen testis dokularında CPF’nin hasar oluşturduğunu ve bu hasar üzerinde kurkuminin antioksidan etkisinin önemli düzeyde var olduğunu ortaya koymaktadır.

Anahtar Kelimeler

Histolojik değişiklikler, Klorprifos, Kurkumin, Rat, Seminifer tübül

Investigation of antioxidant effect of curcumin on testicular tissue damage caused by pesticide chlorpyrifos under light microscopy

Öz

In this study, effect
of commonly used  pesticide
chlorpyrifos’s (CPF) on mammalian testicular tissue damage and antioxidant
curcumin’s effects on damage reducing were investigated. For this purpose, 90
days old 48 male rats (Wistar albino) primarily divided into two groups;
treated for 15 days and treated for 30 days. These groups divided into four
groups within themselves, included; a control, only CPF, CPF + curcumin and
only curcumin groups, respectively. On the 15^th and 30^th
days 6 animals’ testicular tissue from each group were taken and placed in 10%
neutral buffered formalin for histological procedures and evaluated by light
microscopy. It was determined that the CPF group had histopathological changes
such as seminiferous tubule degeneration, reduction of germ cells in
seminiferous tubules, oedema in connective tissue in inter seminiferous tubules
region and necrosis compared to control group. Also, it was observed that CPF
and curcumin group had minor histopathological changes on these tissues
according to only CPF group. There were statistically significant differences
between control group and CPF and CPF + CUR groups as well as between CPF group
and CPF + KUR group (p<0.05). The findings revealed that CPF caused damage in
testicular tissues and curcumin had significant antioxidant effects on  this
damage.

Anahtar Kelimeler

Histological changes, Chlorpyrifos, Curcumin, Rat, Seminiferous tubules, Testicular tissue

Kaynakça

• Abdel-Kawi, S.H., Hassanin, K.M.A., Hassem, K.S., 2016. The effect of high dietary fructose on the kidney of adult albino rats and the role of curcumin supplementation: A biochemical and histological study. Beni-Suef Univ. J. Appl. Sci., 5: 52-60.
• Amri, N., Hammouda, A. Rahmouni, F., Chokri, M.A., Chaabane, R., Selmi, S., Rebai, T., Badraoui, R., 2016. Reproductive effects in hybrid sparrow from a polluted area in Tunisia: Oxidative damage and altered testicular histomorphology. Ecotox. Environ., Safe. 129: 164–170.
• Attia, A:A., El-Mazoudy, R.H., El-Shenawy, N.S., 2012. Antioxidant role of propolis extract against oxidative damage of testicular tissue induced by insecticide chlorpyrifos in rats. Pest.Biochem. Physiol., 103: 87–9.3
• Bancroft, J.D., Stevens, A., 1996. Theory and Practice of Histological Techniques, Fourth Edition,Churcill Livingestone Medical Division of Pearson Professional Limited. Pp. 36. ISBN: 0-443-047-60-X.
• Camougrand, N., Rigoulet, M., 2001. Aging and oxidative Stres: Studies of Some Genes Involved Both in Aging and in Response to Oxidative Stres. Respir. Physiol., 128:3, 393-401.
• Cingöz, Ş., 2013. Kurutma İşleminin Bazı Pestisit Kalıntıları Üzerine Etkisi. Yüksek Lisans Tezi.Gaziosmanpaşa Üniversitesi Fen Bil. Ens. Gıda Mühendisliği A.B.D. Tokat.
• Crumpton, T., Seidler, F., Slotkin, T., 2000. Developmental Neurotoxicity of Chlorpyrifos in vivo and in vitro Effects on Nuclear Transcription Factors Involved in Cell Replication and Differentiation. Brain Res., 857: 87-88.
• Çelik, I., Süzek, H., 2009. Effect of subacute exposure of dichlorus at sublethal dosages on erythrocyte and tissue antioxidant defense systems and lipid peroxidantion in rats. Ecotox. Environ. Safe., 72: 905-908.
• Ceyhun, S.B., Şentürk, M., Ekinci, D., Erdoğan, O., Çiltaş, A., Kocaman, E.M., 2010. Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout. Comp. Biochem. Physiol. C., 152: 215–223.
• Chattopadhyay, I., Biswas, K., Bandyopadhyay, U., Banerjee, R.K., 2004. Turmeric and curcumin: Biological actions and medicinal applications. Curr. Sci., 87(1):44-53.
• Çoban,Ö., Patır, B., 2010. Antioksidan Etkili Bazı Bitki ve Baharatların Gıdalarda Kullanımı. Elect. J. Food Tech., 5(2):7-19.
• Ekinci, D., Beydemir, Ş., 2010. Risk assessment of pesticides and fungicides for acid–base regulation and salt transport in rainbow trout tissues. Pest.Biochem. Physiol., 97: 66–70.
• El-Fattah, A.A, Fahim, A.T., Sadik, N.A.H., Ali, B.M., 2016. Resveratrol and curcumin ameliorate di-(2-ethylhexyl) phthalate induced testicular injury in rats. Gen. Comp. Endocrinol., 225, 45-54.
• El-Mazoudy, R.H., Attia, A., Shenawy, N., 2011. Protective Role of Propolis Against Reproductive Toxicity of Chlorpyrifos in Male Rats. Pest.Biochem. Physiol., 101: 175-181.
• Farag, A., Okazy, A., Asward, A., 2003. Devolopmental Toxicity Study of Chlorpyrifos in Rats. Reprod. Toxicol., 17: 203-208.
• Farag, A., Radwan, A., Sorour, F., El-Okazy, A., El-Agamy, El-S., El-Sebae, Ael-K. 2010. Chlorpyrifos Induced reproductive toxicity in male mice. Reprod. Toxicol., 29, 80-85.
• Ghosh, S., Bhattacharyya, S., Rashid, K.,Sil, P.C., 2015. Curcumin protects rat liver from streptozotocin-induced diabetic pathophysiology by counteracting reactive oxygenspecies and inhibiting the activation of p53 and MAPKs mediated stress response pathways. Toxicol. Rep., 2: 365–376.
• Gültekin, F., Kaleli, S., Altuntaş, İ., Öncü, M., Gökçimen, A., Sütçü, R., 2000. Chlorpyriphos-ethylin rat testis dokusunda in vivo lipoperoksidatif etkisi. Genel Tıp Dergisi, 10 (4) : 147-152.
• Joshi, S.C., Mathur,R., Guluta, N., 2007. Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat. Toxicol. Ind. Health., 23: 439-444.
• Kalender, Y., Kaya, S., Durak, D., Uzun, F.G., Demir, F., 2012. Protective effects of catechin and quercetin on antioxidant status, lipid peroxidation and testis-histoarchitecture induced by chlorpyrifos in male rats. Environ.Toxicol. Phar., 33: 141-148.
• Kierszenbaum, A.L., 2006. Histology and Cell Biology. Mosby. Çeviri: Ramazan Demir, Palme Yayıncılık, Sf: 458-464.
• Kumar, V., Cotran, R.S., Robbins, S.L., 1999. Basic Pathology. WD Saunders Company, Çeviri: Çevikbaş, U. 2000. Temel Patoloji, Nobel Tıp Kitapevleri Ltd. Şti. 4-24.
• Kurutaş, E.B., Kılınç, M., 2003. Pestisitlerin Biyolojik Sistemler Üzerine Etkisi. Arşiv Kaynak Tarama Derg., 12 (3): 215-228.
• Li, M., Liu, C., Yang, L., Zhang, L., Chen, C., He, M., Lu, Y., Feng, W., Pi, H., Zhang, Y., Zhong, M., Yu, Z., Zhou, Z., 2016. G9a-mediated histone methylation regulates cadmium-induced male fertility damage in pubertal mice. Toxicol. Lett., 252: 11–21.
• Ma, P., Wu, Y., Zeng, Q., Gan, Y., Ye, J.X., Yang, X., 2013. Oxidative damage induced by chlorpyrifos in the hepatic andrenal tissue of Kunming mice and the antioxidant role of vitamin E. Food Chem. Toxicol., 58: 177–183.
• Madhavi, M., Madhavi, K., Jithan, A.V., 2012. Preparationand in vitro/in vivo characterization of curcumin microspheres intended to treat colon cancer. J. Pharm. Bioallied. Sci., 4 (2): 164–171.
• Mansour, S.A., Mossa, A.H., 2009. Lipid peroxidation and oxidative stress in rat erythrocytes induced by chlorpyrifos and the protective effect of zinc. Pestic. Biochem. Phys., 93: 34-39.
• Mercan, L., Sirkecioğlu, N., Aksakal, E., Bayır, M., Bayır, A., Aras, M., Ekinci, D., 2013. Goose fat,a promising nutrient for fish feeding, activates antioxidant enzymes in rainbow trout, Oncorhynchus mykiss. Environ. Toxicol. Phar., 36: 964–971.
• Patat S., Akça, H., Kaleli, S., Karakoyun, İ., Koçak, A., Gültekin, F., 2003. Klorprifos–Etil’in HEPG2 Hücre Dizilerinde Hücre Canlılığına Etkisi ve Melatoninin Koruyucu Etkisi. Süleyman Demirel Üniv. Tıp Fak. Der., 10(3): 39-43.
• Reiter, R.J., 1997. Aging and Oxygen Toxicity: Relation to Changes in Melatonin. Age., 20: 201-213.
• Shittu, M., Ayo, J.O., Ambali, S.F., Fatihu, M.Y., Onyeanusi, B.I., Kawu. M.U., 2012. Chronic chlorpyrifos-induced oxidative changes in the testes and pituitary gland of Wistar rats: Ameliorative effects of vitamin C. Pest.Biochem. Physiol., 102:79–85.
• Şıktar, E., Ekinci, D., Şıktar, E., Beydemir, Ş., Gülçin, İ., Günay, M., 2011. Protective role of L-carnitine supplementation against exhaustive exercise induced oxidative stress in rats. Eur. J. Pharmacol., 668: 407–413.
• Thiyagarajan, M., Sharma, S.S., 2004. Neuroprotective Effect of Curcumin in Middle Cerebral Artery Occlusion Induced Focal Cerebral Ischemia in Rats. Life Sci., 74 (8), 969-985.
• Tvrda, E., Tusimova, E., Kovacık, A., Paal, D., Greifova, H., Abdramanov, A., Lukac, N., 2016. Curcumin has protective and antioxidant properties on bulls permatozoa subjected to induced oxidative stress. Anim. Reprod. Sci., 172: 10–20.
• Verma, R.J., Mathuria, N., 2009. Effect of curcumin on aflatoxin-induced biochemical changes in testis of mice. Fertil. Steril., 91(2): 597-601.
• Vural, N., 2005. Toksikoloji. Ankara Üniversitesi Eczacılık Fakültesi Yayınları, No:73, Ankara, Ankara Üniv. Basımevi, ISBN: 975-482-289-1.
• Yousef, M.I., Omar, S.A.M., El-Guandi, M.I., Abdelmegid, L.A., 2010. Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food Chem. Toxicol., 48: 3246-3261.
• Yu, C., Mei, X.T, Zheng, Y.P., Xu, D.H., 2014. Zn(II)–curcumin protects against hemorheologic alterations, oxidative stress and liver injury in a ratmodel of acute alcoholism. Environ.Toxicol. Phar., 37: 729-737.