Yıl 2019, Cilt 34 , Sayı 2, Sayfalar 172 - 183 2019-06-25

Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi
Investigation of microRNA-mediated redox regulation in leaf growth regions during chiling stress tolerance of maize (Zea mays L.)

Fatma Aydinoglu [1] , Burak Akgul [2]


Ilıman iklim tahıllarından olan mısır (Zea mays L.) bitkisi, üşüme stresine oldukça duyarlıdır ve optimum büyüme ve yüksek verim için nispeten yüksek sıcaklığa ihtiyaç duyar. Üşüme stresi, büyüme işlevlerini sekteye uğratmakla birlikte hücrenin redoks homeostazisini bozarak oksidatif strese yol açar. Bu sırada açığa çıkan reaktif oksijen türleri (ROS)’in ise düşük miktarlarda büyümeyi teşvik ettikleri de bilinmektedir. Buradan yola çıkarak, bu çalışmada, mısır fidelerinin üşüme stresine karşı yaprak büyüme cevabının oluşumunda sinyal role sahip reaktif oksijen türlerinin microRNA (miRNA) genleri ile olası regülasyonunun araştırılması amaçlanmıştır.  Bu doğrultuda, ADA 313 adlı mısır hibridi fidelerine üşüme stresi uygulanarak ROS üretimi tetiklenmiştir.  Redoks regülasyonunda başlıca rol oynayan antioksidan genleri ve onları hedef alan microRNA’lar in silico analizlerle belirlenerek, aralarındaki ilişki transkripsiyonel ve enzimatik seviyede gösterilmiştir. Sonuç olarak, yaprak uzama oranı stres koşullarında kontrole göre %19 azalmıştır. SOD 1a genini hedef aldığı tahmin edilen miR528’in stres ve meristem, miR397 ve tahmini hedefi Lakkaz’ın olgunluk spesifik olduğu saptanmıştır. Katalaz (CAT), süperoksit dismutaz (SOD), glutatyon redüktaz (GR), peroksidaz (POX) ve askorbat peroksidaz (APX) antioksidan enzimlerinin aktivitelerinin büyüme bölgeleri arasında farklılık gösterdiği görülmüştür.  Bu çalışma ile ilk defa, mısır bitkisinin üşüme stresine toleransı sırasında yaprak büyüme cevabında oksidatif sinyalizasyonun miRNA genleriyle regülasyonu gösterilmiştir.

Corn (Zea mays L.) is one of the temperate climate crops, very sensitive to chilling stress and needs  relatively high temperature for optimum growth and high yield. Chilling stress disrupts growth processes coupled with disruption of redox homeostasis of the cell, leading to oxidative stress. It is also known that the low amount of reactive oxygen species (ROS) released during this period promote growth. From that view, the aim of this study was to investigate the possible microRNA (miRNA) mediated regulation of ROS playing signalling role in leaf growth response against the chilling stress of maize seedlings. In this respect, ROS production was triggered by applying low night temperature stress to maize hybrid seedlings named ADA313. The antioxidant genes that play a major role in the redox regulation and the microRNAs targeting them were determined by in silico analysis and the relationship between them was validated at the transcriptional and enzymatic level. As a result, the elongation rate of the fourth leaf was reduced by 19% by chilling stress compared to control. miR528, which was predicted to target SOD 1a gene was found meristem and stress specific. The expression of miR397, which was predicted to target Laccase, was detected at maturity. Enzymatic activities of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), peroxidase (POX) and ascorbate peroxidase (APX) were found to differ between the growth regions. In this study, the miRNA-mediated regulation of the oxidative signaling pathway was shown for the first time in the leaf growth zones of maize response to chilling stress.

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Birincil Dil tr
Konular Fen
Bölüm Tarımsal Biyoteknoloji
Yazarlar

Yazar: Fatma Aydinoglu (Sorumlu Yazar)
Kurum: GEBZE TEKNİK ÜNİVERSİTESİ
Ülke: Turkey


Yazar: Burak Akgul
Kurum: GEBZE TEKNİK ÜNİVERSİTESİ
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 25 Haziran 2019

Bibtex @araştırma makalesi { omuanajas482710, journal = {Anadolu Tarım Bilimleri Dergisi}, issn = {1308-8750}, eissn = {1308-8769}, address = {}, publisher = {Ondokuz Mayıs Üniversitesi}, year = {2019}, volume = {34}, pages = {172 - 183}, doi = {10.7161/omuanajas.482710}, title = {Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi}, key = {cite}, author = {Aydinoglu, Fatma and Akgul, Burak} }
APA Aydinoglu, F , Akgul, B . (2019). Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi. Anadolu Tarım Bilimleri Dergisi , 34 (2) , 172-183 . DOI: 10.7161/omuanajas.482710
MLA Aydinoglu, F , Akgul, B . "Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi". Anadolu Tarım Bilimleri Dergisi 34 (2019 ): 172-183 <https://dergipark.org.tr/tr/pub/omuanajas/issue/46215/482710>
Chicago Aydinoglu, F , Akgul, B . "Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi". Anadolu Tarım Bilimleri Dergisi 34 (2019 ): 172-183
RIS TY - JOUR T1 - Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi AU - Fatma Aydinoglu , Burak Akgul Y1 - 2019 PY - 2019 N1 - doi: 10.7161/omuanajas.482710 DO - 10.7161/omuanajas.482710 T2 - Anadolu Tarım Bilimleri Dergisi JF - Journal JO - JOR SP - 172 EP - 183 VL - 34 IS - 2 SN - 1308-8750-1308-8769 M3 - doi: 10.7161/omuanajas.482710 UR - https://doi.org/10.7161/omuanajas.482710 Y2 - 2019 ER -
EndNote %0 Anadolu Tarım Bilimleri Dergisi Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi %A Fatma Aydinoglu , Burak Akgul %T Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi %D 2019 %J Anadolu Tarım Bilimleri Dergisi %P 1308-8750-1308-8769 %V 34 %N 2 %R doi: 10.7161/omuanajas.482710 %U 10.7161/omuanajas.482710
ISNAD Aydinoglu, Fatma , Akgul, Burak . "Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi". Anadolu Tarım Bilimleri Dergisi 34 / 2 (Haziran 2019): 172-183 . https://doi.org/10.7161/omuanajas.482710
AMA Aydinoglu F , Akgul B . Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi. Anadolu Journal of Agricultural Sciences. 2019; 34(2): 172-183.
Vancouver Aydinoglu F , Akgul B . Mısır (Zea mays L.) bitkisinin üşüme stresine toleransı sırasında yaprak büyüme bölgelerinde mikroRNA aracılıklı redoks regülasyonunun incelenmesi. Anadolu Tarım Bilimleri Dergisi. 2019; 34(2): 183-172.