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Uzungöl ve Cernek Göllerindeki (Samsun, Türkiye) Tek Hücreli Siyanobakterilerin İzolasyonu ve Karakterizasyonu Üzerine Araştırmalar

Yıl 2022, Cilt 12, Sayı 2, 167 - 178, 31.12.2022
https://doi.org/10.54370/ordubtd.1205725

Öz

Samsun, Bafra Kızılırmak deltasında yer alan Uzungöl ve Cernek göllerindenden alınan su örneklerinden tek hücreli siyanobakterilerin saf kültürler şeklinde izolasyonu gerçekleştirilmiştir. Seyreltilmiş su örnekleri siyanobakteriler için seçici katı besiyeri yüzeyine ekilerek sürekli ışık altında inkübasyona bırakılmış ve izole koloniler elde edilmiştir. Tek hücrelerden oluşmuş 20 koloni tek hücreli izolatlar olarak seçilmiştir. Her bir izolata ait bu tek koloniler sıvı besiyerlerinde sürekli ışık altında sıvı kültürler şekline üretilmiştir. Bu sıvı kültürlerdeki hücrelere ait karakterler incelenerek hangi siyanobakteriyel altbölüme ve hangi form-cinse dahil oldukları belirlenmiştir. Bu çalışmalar sonucunda bütün izolatların Subsection 1 ve Synechococcus form-cinsine dahil olduğu sonucuna varılmıştır. Bu sonucu desteklemek için her bir izolattan genomik DNA izole edilmiş, 16S rRNA gen bölgesi çoğaltılmış ve her bir amplikon EcoRI ile kesilmiştir. Elde edilen fragment büyüklüklerinin standart Synechococcus sp.PCC7942 suşunun fragment büyüklükleriyle uyumlu olduğu belirlenmiştir. Dolayısıyla restriksiyon fragment büyüklükleri ve kültürel özellikler uyumlu olduğundan izolatların tamamının Altbölüm 1 ve Synechococcus form-cinsinin üyeleri olduğu sonucuna varılmıştır.

Kaynakça

  • AcaClone (2022). pDRAW32 DNA analysis software. Retrieved November 2, 2022 from https://www.acaclone.com/
  • Affe, H. M., Rigonato, J., Nunes, J. M. & Menezes, M. (2018). Metagenomic analysis of cyanobacteria in an oligotrophic tropical estuary, South Atlantic. Frontiers in Microbiology, 9, 1393. https://doi.org/10.3389/fmicb.2018.01393
  • Castenholz, R. W. (2001). General characteristics of the cyanobacteria. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 474-487). Springer.
  • Castenholz, R. W., Wilmotte, A., Herdman, M., Rippka, R., Waterbury, J. B., Iteman, I., & Hoffmann, L. (2001). Phylum BX. Cyanobacteria. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 473-599). Springer. https://doi.org/10.1007/978-0-387-21609-6_27
  • Cyanobase (2022). Retrieved November 2, 2022 from http://genome.microbedb.jp/cyanobase/
  • Gönülol, A., & Çomak, Ö. (1992). Bafra balık gölleri (Balık gölü, Uzungöl) fitoplanktonu üzerinde florikstik araştırmalar I-Cyanophyta. Doğa- Tr. J of Botany, 16, 223–245.
  • Herdman, M., Castenholz, R. W., Iteman, I., Waterbury, J. B., & Rippka, R. (2001a). Subsection I. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 493-514). Springer.
  • Herdman, M., Castenholz, R. W., Iteman, I., Waterbury, J. B. & Rippka, R., (2001b). Form-genus XIII. Synechococcus. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 508-512). Springer.
  • Komárek, J. (2016). A polyphasic approach for the taxonomy of cyanobacteria: Principles and applications. European Journal of Phycology, 51(3), 346-353. https://doi.org/10.1080/09670262.2016.1163738
  • Oren, A. (2004). A proposal for further integration of the cyanobacteria under the Bacteriological Code. International journal of systematic and evolutionary microbiology, 54(5), 1895-1902. https://doi.org/10.1099/ijs.0.03008-0
  • Moreira, C., Martins, J., Vasconcelos, V., & Antunes, A. (2020). Genomics perspectives on cyanobacteria research. In Handbook of Algal Science, Technology and Medicine (pp. 147-159). Academic Press. https://doi.org/10.1016/B978-0-12-818305-2.00009-7
  • Palinska, K. A., & Surosz W. (2014). Taxonomy of cyanobacteria: a contribution to consensus approach. Hydrobiologia, 740, 1–11. https://doi.org/10.1007/s10750-014-1971-9
  • Pang, K., Tang, Q., Chen, L., Wan, B., Niu, C., Yuan, X., & Xiao, S. (2018). Nitrogen-fixing heterocystous cyanobacteria in the Tonian period. Current Biology, 28(4), 616-622. https://doi.org/10.1016/j.cub.2018.01.008
  • Rippka, R., (1988). Isolation and putrification of Cyanobacteria. In L. Parker & A. N. Glazer (Eds.), Methods of Enzymology Vol.167 (pp. 3-27). Elsevier. https://doi.org/10.1016/0076-6879(88)67004-2
  • Rippka, R., Castenholz, R. W., & Herdman, M. (2001a). Form genus III. Cyanobacterium Rippka and Cohen-Bazire 1983. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 497-498). Springer.
  • Rippka, R., Castenholz, R. W., & Herdman, M. (2001b). Form genus IV. Cyanobium Rippka and Cohen-Bazire 1983. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 498-499). Springer.
  • Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., & Stanier, R. Y. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology, 111(1), 1-61. https://doi.org/10.1099/00221287-111-1-1
  • Sambrook, J., & Russell, D. W. (2001). Molecular cloning: A laboratory manual (3rd ed.). Cold Spring Harbor Laboratory Press.
  • Schoch, C. L., Ciufo, S., Domrachev, M., Hotton, C. L., Kannan, S., Khovanskaya, R., Leipe, D., Mcveigh, R., O’Neill, K., Robbertse, B., Sharma, S., Soussov, V., Sullivan, J. P., Sun, L., Turner, S., & Karsch-Mizrachi, I. (2020). NCBI Taxonomy: A comprehensive update on curation, resources and tools. Database, 2020, baaa062, 1-21. https://doi.org/10.1093/database/baaa062
  • Stanier, R. Y., Sistrom, W. R., Hansen, T. A., Whitton, B. A., Castenholz, R. W., Pfennig, N., Gorlenko, V. N., Kondratieva, E. N., Eimhjellen, K. E., Whittenbury, R., Gherna, R. I., & Trüper, H. G. (1978). Proposal to place the nomenclature of the Cyanobacteria (Blue-Green Algae) under the rules of International Code of Nomenclature of Bacteria. International Journal of Systematic and Evolutionary Microbiology, 28(2), 335-336. https://doi.org/10.1099/00207713-28-2-335
  • Stanier R. Y., & Cohen-Bazire G., (1977). Phototrophic prokaryotes: The cyanobacteria. Annual Review of Microbiology, 31, 225-2774. https://doi.org/10.1146/annurev.mi.31.100177.001301
  • Tas, B., & Gonulol, A., (2007). An ecologic and taxonomic study on phytoplankton of a shallow lake, Turkey. Journal of Environmental Biology, 28(2), 439-445. http://www.jeb.co.in/journal_issues/200704_apr07_supp/paper_15.pdf
  • Taton, A., Grubisic, S., Brambilla, E., Wit, R., & Wilmotte, A. (2003). Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): A morphological and molecular approach. Applied and Environmental Microbiology, 69(9), 5157-5169. https://doi.org/10.1128/AEM.69.9.5157-5169.2003
  • Whitton, B. A. (1992). Diversity, ecology and taxonomy of cyanobacteria. In: N. H. Mann & N. G. Carr, (Eds.), Photosynthetic Prokaryotes (pp. 1-37). Plenum Press. https://doi.org/10.1007/978-1-4757-1332-9_1
  • Whitton, B. A., & Potts, M. (2012). Introduction to Cyanobacteria. In B. A. Whitton (Eds.), The ecology of Cyanobacteria II: Their diversity in time and space (pp. 1-13). Kluwer Academic. https://doi.org/10.1007/978-94-007-3855-3_1
  • Wilmotte, A. (1994). Molecular evolution and taxonomy of Cyanobacteria. In: D. A. Bryant (Eds.), The molecular biology of Cyanobacteria (pp. 2-25). Springer. https://doi.org/10.1007/978-94-011-0227-8_1
  • Wilmotte, A., & Herdman, M. (2001). Phylogenetic relationships among the cyanobacteria based on 16S rRNA sequences. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 487-493). Springer.
  • Wolk, C. P. (1996). Heterocyst formation. Annual Review of Genetics, 30(1), 59-78. https://doi.org/10.1146/annurev.genet.30.1.59

Investigations on Isolation and Characterisation of Unicellular Cyanobacteria in Uzungöl and Cernek Lakes, Samsun, Türkiye

Yıl 2022, Cilt 12, Sayı 2, 167 - 178, 31.12.2022
https://doi.org/10.54370/ordubtd.1205725

Öz

Isolation of unicellular cyanobacteria as pure cultures were carried out from the water samples collected from Uzungöl and Cernek lakes in Kızılırmak delta, Bafra, Samsun. Diluted water samples were spread onto a solid BG11 medium which was positively selective for cyanobacteria and isolated single colonies were observed after incubation under continuous light. Twenty colonies composed of unicellular cells were selected as unicellular isolates. These single colonies from each isolates were transferred into a liquid BG11 medium to grow as liquid cultures under continuous light. Characteristics of the cells in liquid cultures of each isolate were used to ıdentify subsection and form-genus of Cyanobacteria they might be included. The results showed that all the isolates were settled in Subsection 1 and form-genus Synechococcus. To support this identification, genomic DNAs were isolated from each of the isolates, 16S rRNA gene regions were amplified, and each amplicon was digested with EcoRI. Restriction fragment lengths were found to be consistent with those of the standard cyanobacterial strain Synechococcus sp. PCC7942. Since restriction fragment lengths and cultural characteristics were consistent, all the isolates were, therefore, evaluated to be included into Subsection 1 and form-genus Synechococcus.

Kaynakça

  • AcaClone (2022). pDRAW32 DNA analysis software. Retrieved November 2, 2022 from https://www.acaclone.com/
  • Affe, H. M., Rigonato, J., Nunes, J. M. & Menezes, M. (2018). Metagenomic analysis of cyanobacteria in an oligotrophic tropical estuary, South Atlantic. Frontiers in Microbiology, 9, 1393. https://doi.org/10.3389/fmicb.2018.01393
  • Castenholz, R. W. (2001). General characteristics of the cyanobacteria. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 474-487). Springer.
  • Castenholz, R. W., Wilmotte, A., Herdman, M., Rippka, R., Waterbury, J. B., Iteman, I., & Hoffmann, L. (2001). Phylum BX. Cyanobacteria. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 473-599). Springer. https://doi.org/10.1007/978-0-387-21609-6_27
  • Cyanobase (2022). Retrieved November 2, 2022 from http://genome.microbedb.jp/cyanobase/
  • Gönülol, A., & Çomak, Ö. (1992). Bafra balık gölleri (Balık gölü, Uzungöl) fitoplanktonu üzerinde florikstik araştırmalar I-Cyanophyta. Doğa- Tr. J of Botany, 16, 223–245.
  • Herdman, M., Castenholz, R. W., Iteman, I., Waterbury, J. B., & Rippka, R. (2001a). Subsection I. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 493-514). Springer.
  • Herdman, M., Castenholz, R. W., Iteman, I., Waterbury, J. B. & Rippka, R., (2001b). Form-genus XIII. Synechococcus. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 508-512). Springer.
  • Komárek, J. (2016). A polyphasic approach for the taxonomy of cyanobacteria: Principles and applications. European Journal of Phycology, 51(3), 346-353. https://doi.org/10.1080/09670262.2016.1163738
  • Oren, A. (2004). A proposal for further integration of the cyanobacteria under the Bacteriological Code. International journal of systematic and evolutionary microbiology, 54(5), 1895-1902. https://doi.org/10.1099/ijs.0.03008-0
  • Moreira, C., Martins, J., Vasconcelos, V., & Antunes, A. (2020). Genomics perspectives on cyanobacteria research. In Handbook of Algal Science, Technology and Medicine (pp. 147-159). Academic Press. https://doi.org/10.1016/B978-0-12-818305-2.00009-7
  • Palinska, K. A., & Surosz W. (2014). Taxonomy of cyanobacteria: a contribution to consensus approach. Hydrobiologia, 740, 1–11. https://doi.org/10.1007/s10750-014-1971-9
  • Pang, K., Tang, Q., Chen, L., Wan, B., Niu, C., Yuan, X., & Xiao, S. (2018). Nitrogen-fixing heterocystous cyanobacteria in the Tonian period. Current Biology, 28(4), 616-622. https://doi.org/10.1016/j.cub.2018.01.008
  • Rippka, R., (1988). Isolation and putrification of Cyanobacteria. In L. Parker & A. N. Glazer (Eds.), Methods of Enzymology Vol.167 (pp. 3-27). Elsevier. https://doi.org/10.1016/0076-6879(88)67004-2
  • Rippka, R., Castenholz, R. W., & Herdman, M. (2001a). Form genus III. Cyanobacterium Rippka and Cohen-Bazire 1983. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 497-498). Springer.
  • Rippka, R., Castenholz, R. W., & Herdman, M. (2001b). Form genus IV. Cyanobium Rippka and Cohen-Bazire 1983. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 498-499). Springer.
  • Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., & Stanier, R. Y. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology, 111(1), 1-61. https://doi.org/10.1099/00221287-111-1-1
  • Sambrook, J., & Russell, D. W. (2001). Molecular cloning: A laboratory manual (3rd ed.). Cold Spring Harbor Laboratory Press.
  • Schoch, C. L., Ciufo, S., Domrachev, M., Hotton, C. L., Kannan, S., Khovanskaya, R., Leipe, D., Mcveigh, R., O’Neill, K., Robbertse, B., Sharma, S., Soussov, V., Sullivan, J. P., Sun, L., Turner, S., & Karsch-Mizrachi, I. (2020). NCBI Taxonomy: A comprehensive update on curation, resources and tools. Database, 2020, baaa062, 1-21. https://doi.org/10.1093/database/baaa062
  • Stanier, R. Y., Sistrom, W. R., Hansen, T. A., Whitton, B. A., Castenholz, R. W., Pfennig, N., Gorlenko, V. N., Kondratieva, E. N., Eimhjellen, K. E., Whittenbury, R., Gherna, R. I., & Trüper, H. G. (1978). Proposal to place the nomenclature of the Cyanobacteria (Blue-Green Algae) under the rules of International Code of Nomenclature of Bacteria. International Journal of Systematic and Evolutionary Microbiology, 28(2), 335-336. https://doi.org/10.1099/00207713-28-2-335
  • Stanier R. Y., & Cohen-Bazire G., (1977). Phototrophic prokaryotes: The cyanobacteria. Annual Review of Microbiology, 31, 225-2774. https://doi.org/10.1146/annurev.mi.31.100177.001301
  • Tas, B., & Gonulol, A., (2007). An ecologic and taxonomic study on phytoplankton of a shallow lake, Turkey. Journal of Environmental Biology, 28(2), 439-445. http://www.jeb.co.in/journal_issues/200704_apr07_supp/paper_15.pdf
  • Taton, A., Grubisic, S., Brambilla, E., Wit, R., & Wilmotte, A. (2003). Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): A morphological and molecular approach. Applied and Environmental Microbiology, 69(9), 5157-5169. https://doi.org/10.1128/AEM.69.9.5157-5169.2003
  • Whitton, B. A. (1992). Diversity, ecology and taxonomy of cyanobacteria. In: N. H. Mann & N. G. Carr, (Eds.), Photosynthetic Prokaryotes (pp. 1-37). Plenum Press. https://doi.org/10.1007/978-1-4757-1332-9_1
  • Whitton, B. A., & Potts, M. (2012). Introduction to Cyanobacteria. In B. A. Whitton (Eds.), The ecology of Cyanobacteria II: Their diversity in time and space (pp. 1-13). Kluwer Academic. https://doi.org/10.1007/978-94-007-3855-3_1
  • Wilmotte, A. (1994). Molecular evolution and taxonomy of Cyanobacteria. In: D. A. Bryant (Eds.), The molecular biology of Cyanobacteria (pp. 2-25). Springer. https://doi.org/10.1007/978-94-011-0227-8_1
  • Wilmotte, A., & Herdman, M. (2001). Phylogenetic relationships among the cyanobacteria based on 16S rRNA sequences. In R. D. Boone & R. W. Castenholz (Eds.), Bergey's manual of systematic bacteriology Vol. 1: The Archaea and the deeply branching and phototrophic Bacteria (pp. 487-493). Springer.
  • Wolk, C. P. (1996). Heterocyst formation. Annual Review of Genetics, 30(1), 59-78. https://doi.org/10.1146/annurev.genet.30.1.59

Ayrıntılar

Birincil Dil İngilizce
Konular Temel Bilimler
Bölüm Araştırma Makaleleri
Yazarlar

Kübra ÖZKUL>
Bir kuruma bağlı değildir
0000-0001-8958-4185
Türkiye


Haydar KARAKAYA> (Sorumlu Yazar)
ONDOKUZ MAYIS ÜNİVERSİTESİ
0000-0003-3347-0001
Türkiye

Destekleyen Kurum Ondokuz Mayıs Üniversitesi
Proje Numarası F373
Teşekkür Yazarlar F373 nolu proje ile bu çalışmayı destekleyen Ondokuz Mayıs Üniversitesine teşekkür ederler.
Yayımlanma Tarihi 31 Aralık 2022
Yayınlandığı Sayı Yıl 2022, Cilt 12, Sayı 2

Kaynak Göster

APA Özkul, K. & Karakaya, H. (2022). Investigations on Isolation and Characterisation of Unicellular Cyanobacteria in Uzungöl and Cernek Lakes, Samsun, Türkiye . Ordu Üniversitesi Bilim ve Teknoloji Dergisi , 12 (2) , 167-178 . DOI: 10.54370/ordubtd.1205725