The relationship between short term fasting and oxidative stress in pediatric emergency patients

Yıl 2019, , 378 - 382, 01.12.2019

Halit Halil , Can Demir Karacan

https://doi.org/10.21601/ortadogutipdergisi.487827

Öz

Aim: Healthy life is sustained by maintaining the balance between oxidative and antioxidant homeostasis in the living organism. During oxidation process free radical products lead to cellular damage and death. It is well known that oxidative stress plays role in the etiology of many diseases. We aimed to study the relationship between oxidative stress and short term fasting by measuring the serum levels of ischemia modified albumin, thiol, and disulphide in pediatrics patients admitted for radiological imaging.
Material and Method: Our study was carried out between April and November 2018. We included children between 6 months and 6 years. Children who were fasting for more than 5 hours were assigned as the study group. Children who were not fasting were assigned as the control group. Serum ischemia modified albumin, thiol and disulfide were calculated using the colorimetric method of Erel’s.
Results: In the study group, the mean± SD serum level of ischemia modified albumin, native thiol, total thiol and disulphide were 0.72± 0.12 ABSU, 559.15± 58.98 μmol/L, 606.83± 61.35 μmol/L and 23.83± 5.58 μmol/L respectively. In the control group, the mean ± SD serum level of ischemia modified albumin, native thiol, total thiol and disulphide were 0.77± 0.15 ABSU, 528.80± 54.45 μmol/L, 580.17± 63.41 μmol/L and 25.68± 8.07 μmol/L respectively. The disulphide /native thiol and disulphide / total thiol ratios were lower in the study group than the controls, but there were no significant differences between the two groups.
Conclusion: Short term fasting does not change serum levels of oxidative stress biomarkers and dose not lead to inbalance in the oxidative stress hemeostasis in pediatric patients.

Anahtar Kelimeler

disulphide, fasting, ischemia modified albumin, pediatrics, thiol, oxidative stress

Acil servise başvuran çocuk hastalarda kısa süreli açlık ile oksidatif stres arasındaki ilişkinin değerlendirilmesi

Öz

Amaç: Sağlıklı bir yaşamın devam ettirilebilmesi için organizmanın oksidan antioksidan dengesinin korunması gereklidir. Oksidatif stresteki artış sonucunda oluşan serbest radikal türleri hücre zedelenmesine ve hücre ölümüne neden olur. Oksidatif stresin birçok hastalığın etiyolojisinde rolü olduğu bilinmektedir. Bu çalışmanın amacı radyolojik görüntüleme için kısa süre aç bırakılan çocuk hastaların iskemi modifiye albumin, tiyol ve disulfid serum düzeylerini belirleyerek, açlık ile oksidatif stres arasındaki ilişkiyi araştırmaktır.
Gereç ve Yöntem: Çalışmamız Nisan ile Kasım 2018 tarihleri arasında gerçekleştirildi. Yaşları altı ay ile 6 yaş arasında değişen pediatrik hastalar dahil edildi. En az beş saat aç bırakılan pediatrik hastalar çalışma grubu olarak belirlendi, aç olmayan hastalar kontrol grubu olarak kabul edildi. Oksidatif stres parametreleri olarak iskemi modifiye albumin, tiyol ve disülfid serum düzeylerini Erel kalorimetrik yöntemi ile ölçüldü.
Bulgular: En az beş saat aç olan hastaların ortalama ± SD nativ tiyol düzeyi 559,15± 58,98 μmol/L, total tiyol düzeyi 606,83± 61,35μmol/L, disülfid düzeyi 23,83± 5,58 μmol/L ve iskemi modifiye albumin düzeyi 0,72± 0,12 ABSU idi. Kontrol hastalarında ortalama± SD nativ tiyol düzeyi 528,80± 54,45 μmol/L, total tiyol düzeyi 580,17± 63,41 μmol/L, disülfid düzeyi 25,68± 8,07 μmol/L ve iskemi modifiye albumin 0,77± 0,15 ABSU idi. Disülfid/tiyol ve disülfid/total tiyol oranları aç bırakılan hasta grubunda konrol grubuna göre daha düşüktü, ayrıca gruplar arasında oranların ortalamaları açısından anlamlı bir istatistiksel farklılık saptanmadı.
Sonuç: Kısa süre aç bırakılan çocuklarda oksidatif stres belirteçlerinin değişmediği ve dengesinin bozulmadığı görüşüne varıldı.

Anahtar Kelimeler

açlık, çocuk, disülfid, iskemi modifiye albumin, tiyol, oksidatif stres

Kaynakça

• Jisa KA, Clarey DD, Peeples ES. Magnetic Resonance Imaging Findings of Term and Preterm Hypoxic-Ischemic Encephalopathy: A Review of Relevant Animal Models and Correlation to Human Imaging. Open Neuroimag J. 2018 Oct 17; 12: 55-65. (doi: 10.2174/1874440001812010055). eCollection 2018.
• Mürner-Lavanchy IM, Kidokoro H, Thompson DK, Doyle LW, Cheong JLY, Hunt RW, Inder TE, Anderson PJ. Thirteen-Year Outcomes in Very Preterm Children Associated with Diffuse Excessive High Signal Intensity on Neonatal Magnetic Resonance Imaging. J Pediatr. 2018 Nov 7. pii: S0022-3476(18)31428-8. (doi: 10.1016/j.jpeds.2018.10.016).
• Jain S. Sedation: A Primer for Pediatricians. Pediatr Ann. 2018 Jun 1; 47(6): 254-58. (doi: 10.3928/19382359-20180522-04).
• Ramalho CE, Bretas PMC, Schvartsman C, Reis AG. Sedation and analgesia for procedures in the pediatric emergency room. J Pediatr (Rio J). 2017 Nov - Dec; 93 Suppl 1: 2-18. (doi: 10.1016/j.jped.2017.07.009). Epub 2017 Sep 23.
• Wu P, Chu W, Liu X, Guo X, Zhang J. The Influence of Short-term Fasting on Muscle Growth and FiberHypotrophy Regulated by the Rhythmic Expression of Clock Genes and Myogenic Factors in Nile Tilapia. Mar Biotechnol (NY). 2018 Dec; 20(6): 750-68. (doi: 10.1007/s10126-018-9846-0).
• Salin K, Villasevil EM, Anderson GJ, Auer SK, Selman C, Hartley RC, Mullen W, Chinopoulos C, Metcalfe NB. Decreased mitochondrial metabolic requirements in fasting animals carry an oxidative cost. Funct Ecol. 2018 Sep; 32(9): 2149-57. (doi: 10.1111/1365-2435.13125). Epub 2018 May 29.
• Jongbloed F, Saat TC, Verweij M, Payan-Gomez C, Hoeijmakers JH, van den Engel S, van Oostrom CT, Ambagtsheer G, Imholz S, Pennings JL, van Steeg H, IJzermans JN, Dollé ME, de Bruin RW. A signature of renal stress resistance induced by short-term dietary restriction, fasting, and protein restriction. Sci Rep. 2017 Jan 19; 7: 40901. (doi: 10.1038/srep40901).
• Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive Oxygen Species in Inflammation and Tissue Injury. Antioxid Redox Signal. 2014 Mar 1; 20(7): 1126–67. (doi:10.1089 /ars. 2012.5149).
• Khan HA, Alhomida AS, Sobki SH, Habib SS, Al Aseri Z, Khan AA, Al Moghairi A. Serum markers of tissue damage and oxidative stress in patients with acute myocardial infarction. Biomedical Research 2013; 24(1): 15-20.
• Coverdale JPC, Katundu KGH, Sobczak AIS, Arya S, Blindauer CA, Stewart AJ. Ischemia-modified albumin: Crosstalk between fatty acid and cobalt binding. Prostaglandins Leukot Essent Fatty Acids. 2018 Aug; 135: 147-57. (doi: 10.1016/j.plefa.2018.07.014). Epub 2018 Jul 20.
• Baba SP, Bhatnagar A. Role of thıols ın oxıdatıve stress. Curr Opin Toxicol. 2018 Feb; 7: 133-39. (doi: 10.1016/j.cotox.2018.03.005). Epub 2018 Mar 21.
• Gündüzöz M, Birgin İritaş S, Tutkun L, Büyükşekerci M, Pinar Çetintepe S, Bal C, Alişik M, Erdoğan S, Yilmaz H, Erel Ö. A new potential biomarker in early diagnosis of firefighter lung function impairment: dynamic thiol / disulphide homeostasis. Cent Eur J Public Health. 2018 Sep; 26(3): 190-94. (doi: 10.21101/cejph.a4972).
• Sinha N, Dabla PK. Oxidative stress and antioxidants in hypertension-a current review. Curr Hypertens Rev. 2015; 11(2): 132-42.
• Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004; 37: 112– 19.
• Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38: 1103– 11.
• Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014 Dec; 47(18): 326-32.
• Green SM, Krauss B. Pulmonary aspiration risk during emergency department procedural sedation – An examination of the role of fasting and sedation depth. Acad Emerg Med. 2002; 9: 35–42.