Can New Advanced Clinical Parameters Be Used in the Diagnosis of Vitamin B12 Deficiency?

Öz

The measurement of vitamin B12 levels has low sensitivity for the diagnosis of vitamin B12 deficiency, therefore, clinicians may prefer to decide the deficiency with the patient's clinic. In this study, the diagnostic values of the classical and advanced clinical complete blood count parameters were investigated to find a new marker in the diagnosis of vitamin B12 deficiency. 150 adult volunteers were included in the study and volunteers were divided into two groups according to their vitamin B12 levels (with or without vitamin B12 deficiency) and hemoglobin levels (with or without anemia). The differences and correlations of laboratory test results between groups were examined. Equivalent of reticulocyte hemoglobin, equivalent of erythrocyte hemoglobin and delta-hemoglobin were found significant differences between groups with or without vitamin B12 deficiency. Hypo-hemoglobinised red cells and hyper-hemoglobinised red cells were found significant difference between groups with or without anemia. Significant positive correlations were found between vitamin B12 levels and equivalent of reticulocyte hemoglobin, equivalent of erythrocyte hemoglobin and delta-hemoglobin. Significant positive correlations were found between hemoglobin levels and macrocytic red blood cells, equivalent of reticulocyte hemoglobin, equivalent of erythrocyte hemoglobin, delta hemoglobin and hyper-hemoglobinised red cells; negative correlations with microcytic red blood cells and hypo-hemoglobinised red cells. It is suggested that these advanced clinical parameters of complete blood count analysis may be used to determine vitamin B12 deficiency.

Anahtar Kelimeler

• Vitamin B12 deficiency
• Anemia
• Complete Blood Count
• Erythrocytes
• Reticulocytes

B12 Vitamini Eksikliğinin Tanısında Yeni Gelişmiş Klinik Parametreler Kullanılabilir mi?

Öz

B12 vitamini düzeylerinin ölçümünün B12 vitamini eksikliği tanısında duyarlılığı düşüktür, bu nedenle, klinisyenler eksikliğe hastanın kliniği ile karar vermeyi tercih edebilir. Bu çalışmada B12 vitamini eksikliği tanısında yeni bir belirteç bulmak amacıyla klasik ve ileri klinik tam kan sayımı parametrelerinin tanısal değerleri araştırıldı. Çalışmaya 150 yetişkin gönüllü dahil edildi ve gönüllüler B12 vitamini düzeylerine (B12 vitamini eksikliği olan veya olmayan) ve hemoglobin düzeylerine (kansızlık olan veya olmayan) göre iki gruba ayrıldı. Laboratuvar test sonuçlarının gruplar arasındaki farklılıkları ve korelasyonları incelendi. B12 vitamini eksikliği olan ve olmayan gruplar arasında retikülosit hemoglobini, eritrosit hemoglobin ve delta-hemoglobin açısından anlamlı farklar bulundu. Anemisi olan ve olmayan gruplar arasında hipo-hemoglobinize eritrositler ve hiper-hemoglobinize kırmızı hücreler arasında anlamlı fark bulundu. B12 vitamini düzeyleri ile retikülosit hemoglobini, eritrosit hemoglobin ve delta-hemoglobin arasında anlamlı pozitif korelasyon bulundu. Hemoglobin seviyeleri ile makrositik eritrositler, retikülosit hemoglobini, eritrosit hemoglobini, delta hemoglobin ve hiper-hemoglobinize eritrositler arasında anlamlı pozitif korelasyonlar; mikrositik eritrositler ve hipo-hemoglobinize eritrosit düzeyleri ile negatif korelasyonlar bulundu. Tam kan sayımı analizinin bu ileri klinik parametrelerinin B12 vitamini eksikliğini belirlemek için kullanılabileceği öne sürülmektedir.

Anahtar Kelimeler

• B 12 Vitamini Eksikliği
• Anemi
• Tam kan sayımı
• Eritrositler
• Retikülositler

Kaynakça

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