Sıçan hipokampüsünün histolojik katmanlarında EphB2 reseptör ekspresyonu

Öz

Hipokampus, depresyon, Alzheimer hastalığı, Parkinson hastalığı ve epilepsi gibi önemli hastalıklardan etkilenen ana beyin bölgelerinden biridir. Hipokampüsün farklı histolojik alt alanlarının çeşitli patolojik süreçlerden etkilendiği bildirilmektedir. Eph reseptörleri, nöronal plastisite için gerekli süreçlerde yer alan bir transmembran reseptör ailesinin üyeleridir. Bu reseptörler arasında EphB2, hipokampüste LTP'nin düzenlenmesinde hem morfolojik hem de sinaptik plastisitede rol oynar. Bu çalışmada, hipokampal süreçlerde önemli olan EphB2 reseptörünün hipokampüsteki histolojik substratlarda ekspresyon yoğunluklarını araştırmayı amaçladık. Sıçanlardan alınan beyin kesitlerinde EphB2 immünofloresan olarak boyanmış ve hipokampal alanlardaki floresan yoğunlukları değerlendirilmiştir. Sonuçlarımıza göre, EphB2 reseptörlerinin ifade düzeyleri hipokampüsün alt katmanlarında değişiklik göstermektedir. Dentat girus, EphB2 ekspresyonunun en yüksek olduğu anatomik katmandı. Tüm bölgelerde, hücresel katmanlarda diğer katmanlara kıyasla daha yüksek EphB2 kümelenmesi tespit edilmiştir. Farklı katmanlarda EphB2 ekspresyonundaki farklılıklar, çeşitli sinir sistemi patolojilerinde gelişen davranış bozukluklarıyla ilişkili olabilir. Sonuçlarımız, morfolojik ve sinaptik plastisitedeki moleküler yolakların daha iyi anlaşılmasını sağlayacak gelecekteki çalışmalar için faydalı olabilir.

Anahtar Kelimeler

• EphB2 Reseptörü
• Hipokampüs
• İmmünofloresan
• Nöroplastisite
• Reseptör Protein-Tirozin Kinazlar

EphB2 receptor expression in histological layers of rat hippocampus

Öz

The hippocampus is one of the main brain regions affected by major diseases such as depression, Alzheimer's disease, Parkinson's disease, and epilepsy. Different histological sub-areas of the hippocampus are reported to be affected by various pathological processes. Eph receptors are members of a family of transmembrane receptors involved in processes necessary for neuronal plasticity. Among these receptors, EphB2 plays a role in both morphological and synaptic plasticity in regulating LTP in the hippocampus. In this study, we aimed to investigate the expression intensities of the EphB2 receptor, which is essential in hippocampal processes in histological substrates in the hippocampus. In brain sections from rats, EphB2 was immunofluorescently stained, and fluorescence intensities in hippocampal areas were evaluated. According to our results, the expression levels of EphB2 receptors vary in the lower layers of the hippocampus. The dentate gyrus was the anatomical layer with the highest expression of EphB2. In all areas, higher EphB2 clustering was detected in cellular layers compared to other layers. Differences in the expression of EphB2 in different layers may be related to behavioural disorders that develop in various nervous system pathologies. Our results may be useful for future studies that will provide a better understanding of molecular pathways in morphological and synaptic plasticity.

Anahtar Kelimeler

• EphB2 Receptor
• Hippocampus
• Immunofluorescence
• Neuroplasticity
• Receptor Protein-Tyrosine Kinases

Kaynakça

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