Zaman Kısıtlamalı Beslenme Müdahalesi Kolonda İnflammasom Aktivasyonunu Transkripsiyonel Düzenleme Yoluyla Baskılamakta

Öz

Bu çalışma, zaman kısıtlamalı beslenme modelinin kolonik inflamasyonla ilişkili inflammasom gen ekspresyonu üzerindeki etkilerini değerlendirmeyi amaçlamıştır. On iki aylık erkek Wistar sıçanlar 35 gün süreyle kontrol (ad libitum beslenme) veya günlük 18 saat açlık–6 saat beslenme protokolünü içeren aralıklı açlık uygulamasına tabi tutulmuştur (n=7/grup). Deney sonunda kolon dokularından toplam RNA izole edilmiş ve cDNA sentezi sonrası inflammasom kompleksinin temel bileşenleri olan Nlrp3, Asc, Caspase-1 ile proinflamatuar sitokin genleri Il-1β ve Il-18’in ekspresyon düzeyleri kantitatif gerçek zamanlı PCR yöntemiyle analiz edilmiştir. Gen ekspresyon düzeyleri normalize edilmiş ΔCt değerleri üzerinden istatistiksel olarak değerlendirilmiştir. Aralıklı açlık uygulanan grupta tüm hedef genlerde kontrol grubuna kıyasla anlamlı düzeyde transkripsiyonel baskılanma saptanmıştır (p<0.05). Bulgular, zaman kısıtlamalı beslenmenin Nlrp3 inflammasom eksenini üst (sensör/adaptör) ve alt (efektör/sitokin) düzeylerde modüle ederek kolonik inflamatuar yanıtı baskılayabileceğini göstermektedir. Sonuç olarak, aralıklı açlık uygulamasının inflamasyon temelli bağırsak patolojilerinde tamamlayıcı ve koruyucu bir strateji olarak değerlendirilebileceği düşünülmektedir. Ancak bu moleküler düzeydeki bulguların fonksiyonel ve klinik çalışmalarla desteklenmesi gerekmektedir.

Anahtar Kelimeler

• Aralıklı oruç
• NLRP3 inflamasyon
• kolonik inflamasyon
• Wistar rat.

Time-Restricted Nutritional Intervention Suppresses Inflammasome Activation in the Colon Through Transcriptional Regulation

Öz

This study aimed to evaluate the effects of a time-restricted feeding model on inflammasome-related gene expression associated with colonic inflammation. Twelve-month-old male Wistar rats were assigned either to a control group (ad libitum feeding) or to an intermittent fasting protocol consisting of 18 hours of daily fasting followed by 6 hours of feeding for 35 days (n = 7 per group). At the end of the experimental period, total RNA was isolated from colon tissues, and following cDNA synthesis, the expression levels of core inflammasome components (Nlrp3, Asc, and Caspase-1) and pro-inflammatory cytokine genes (Il-1β and Il-18) were analyzed using quantitative real-time PCR. Gene expression levels were statistically evaluated based on normalized ΔCt values. The intermittent fasting group exhibited significant transcriptional downregulation of all target genes compared to controls (p < 0.05). These findings indicate that time-restricted feeding may suppress colonic inflammatory responses by modulating the Nlrp3 inflammasome axis at both upstream (sensor/adaptor) and downstream (effector/cytokine) levels. Collectively, the results suggest that intermittent fasting may represent a complementary and preventive strategy in inflammation-based intestinal pathologies. Nevertheless, the molecular findings reported here require further validation through functional and clinical investigations.

Anahtar Kelimeler

• Intermittent fasting
• NLRP3 inflammasome
• colonic inflammation
• Wistar rat.

Kaynakça

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