STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats

Tuğba Semerci Sevimli; Murat Sevimli; İbrahim Onaran; Dilek Bayram

doi:10.20515/otd.04872

STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats

Öz

Özet: Silibinin, Silybum marianum (Deve dikeni) adı verilen bitkiden elde edilen bir flavanoiddir. Bu deneysel çalışmada; hücre koruyucu, antiinflamatuar, antikanserojen, antioksidan etkileri olan ve proteüniriyi azalttığı bilinen Silibininin, STZ ile diyabet oluşturulan ratların böbreklerinde HIF-1α ve TLR2 genlerinin ekspresyon seviyelerine etkisini tedavi öncesi ve sonrası karşılaştırıp, histokimyasal olarak da inceledik. Çalışmamızda 5 grup vardı: Kontrol Grubu, Diyabet Grubu (STZ 65 mg/kg, i.p. tek doz), Diyabetik Grup+100mg/kg Silibinin Tedavi Grubu, Diyabetik Grup+200mg/kg Silibinin Tedavi Grubu, Silibinin grubu (5 rata 100 mg/kg silibinin, 5 rata 200 mg/kg silibinin). Böbreğin biri HIF-1α ve TLR2 genlerinin ekspresyon seviyeleri için değerlendirilirken, diğer böbrek histopatolojik olarak değerlendirildi. Histolojik olarak ilaç alan gruplarda, diyabetik gruplara kıyasla Diyabetik Nefropati (DN) bulguları açısından anlamlı bir değişiklik gözlenmedi. Alınan doku örneklerinden elde edilen total RNA’lar kullanılarak RT-PCR metodu ile 2 adet hedef gen ve bir adet kalibratör genin ifadesindeki değişiklikler incelendi. Elde edilen bulgular kontrol grubu ve diğer gruplardaki örnekler ile karşılaştırılmıştır. 2-ΔΔCT yöntemi ile kat değişimleri hesaplandığında ve eşik -2/2 olarak alındığında TLR2 geninde değişimler tespit edilmiştir ancak istatistiksel olarak anlamlı bir fark bulunamamıştır (p< 0,05). Silibinin’in diyabetli ratların böbrek dokusunda oluşan hasara karşı yeteri kadar etkili olmadığı görüldü.

Anahtar Kelimeler: Diyabet, Silibinin, HIF-1α, TLR2

Abstract: Silibinin is a flavonoid that derived from the plant named Silybum marianum.In this experimental study, we compared and histochemically examined the effect of Silibinin on the expression levels of HIF-1α and TLR2 genes in the kidneys of STZ-diabetic rats before and after the treatment. There were 5 groups in this study: Control Group, Diabetes Group (STZ 65 mg/kg, i.p. single dose), Diabetic Group+100mg/kg Silibinin Treatment Group, Diabetic Group +200mg/kg Silibinin Treatment Group, Silibinin group (100 mg/kg silibinin on 5 rats, 200 mg/kg silibinin on 5 rats). The changes in the expression of two target genes and one calibrator gene were examined using the RT-PCR method. No statistically significant difference was found when the fold changes were calculated using 2-ΔΔCT method (p>0,05). No significant change was observed in the groups taking drugs histologically when compared to diabetic groups. It was seen that Silibinin is not quite effective on the damage formed in the kidney tissue of diabetic rats according to Diabetic Nephropaty findings. Also silibinin is not effective on the mRNA levels of diabetic rat kidneys.

Keywords: Diabetes, Silibinin, HIF-1α, TLR2

Anahtar Kelimeler

diyabet,silibinin,HIF-1α

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Tuğba Semerci Sevimli
SULEYMAN DEMIREL UNIV
Türkiye

Murat Sevimli Bu kişi benim
SULEYMAN DEMIREL UNIV
Türkiye

İbrahim Onaran Bu kişi benim
SULEYMAN DEMIREL UNIV
Türkiye

Dilek Bayram Bu kişi benim
SULEYMAN DEMIREL UNIV
Türkiye

Yayımlanma Tarihi

7 Ocak 2017

Gönderilme Tarihi

9 Ocak 2017

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2017 Cilt: 39 Sayı: 1

DOI

https://doi.org/10.20515/otd.04872

IZ

https://izlik.org/JA64TN27ZE

Kaynak Göster

RIS / Bibtex

APA

Sevimli, T. S., Sevimli, M., Onaran, İ., & Bayram, D. (2017). STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats. Osmangazi Tıp Dergisi, 39(1), 6-12. https://doi.org/10.20515/otd.04872

AMA

1.Sevimli TS, Sevimli M, Onaran İ, Bayram D. STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats. Osmangazi Tıp Dergisi. 2017;39(1):6-12. doi:10.20515/otd.04872

Chicago

Sevimli, Tuğba Semerci, Murat Sevimli, İbrahim Onaran, ve Dilek Bayram. 2017. “STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats”. Osmangazi Tıp Dergisi 39 (1): 6-12. https://doi.org/10.20515/otd.04872.

EndNote

Sevimli TS, Sevimli M, Onaran İ, Bayram D (01 Ocak 2017) STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats. Osmangazi Tıp Dergisi 39 1 6–12.

IEEE

[1]T. S. Sevimli, M. Sevimli, İ. Onaran, ve D. Bayram, “STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats”, Osmangazi Tıp Dergisi, c. 39, sy 1, ss. 6–12, Oca. 2017, doi: 10.20515/otd.04872.

ISNAD

Sevimli, Tuğba Semerci - Sevimli, Murat - Onaran, İbrahim - Bayram, Dilek. “STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats”. Osmangazi Tıp Dergisi 39/1 (01 Ocak 2017): 6-12. https://doi.org/10.20515/otd.04872.

JAMA

1.Sevimli TS, Sevimli M, Onaran İ, Bayram D. STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats. Osmangazi Tıp Dergisi. 2017;39:6–12.

MLA

Sevimli, Tuğba Semerci, vd. “STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats”. Osmangazi Tıp Dergisi, c. 39, sy 1, Ocak 2017, ss. 6-12, doi:10.20515/otd.04872.

Vancouver

1.Tuğba Semerci Sevimli, Murat Sevimli, İbrahim Onaran, Dilek Bayram. STZ (Streptozotosin) ile Diyabet Oluşturulmuş Ratların Böbrek Dokusunda Silibinin’in HIF-1α (Hypoxıa-InducıbleFactor-1 Alpha) ve TLR-2 (Toll Lıke Receptor 2) Genlerinin mRNA Düzeylerine Etkisi/Effect Of Silibinin On Mrna Levels Of Hif-1α (Hypoxia-Inducible Factor-1 Alpha) And Tlr2 (Toll-Like Receptor 2) Genes In Kidney of Stz (Streptozotocin) Induced Diabetic Rats. Osmangazi Tıp Dergisi. 01 Ocak 2017;39(1):6-12. doi:10.20515/otd.04872