Mirtazapin Diyabet Oluşturulan Sıçanların Beyinciklerindeki Hasarı Kısmen Düzeltmektedir

Yıl 2018, Cilt: 40 Sayı: 3, 61 - 69, 01.09.2018

Erhan Şahin Ezgi Bektur Dilek Burukoğlu Dönmez Cengiz Bayçu Devrim Özgür Can Varol Şahintürk

https://doi.org/10.20515/otd.394800

Öz

Diabetes mellitus sistemik bir
hastalık olup çeşitli organlarda hasara neden olmaktadır. Bu organlar arasında beyincik
de yer almaktadır. Mirtazapin ise majör depresyon tedavisinde kullanılan bir
antidepresan madde olup inflamasyon yanıtının düzenlenmesinde de rol
oynamaktadır.  Bütün bu bilgiler ışığında, çalışmamızda DM oluşturulan sıçanların
beyinciklerinde meydana gelen değişiklikleri ve bu değişiklikler üzerine
mirtazapinin etkilerini araştırmayı amaçladık. Çalışmamızda toplam 21 adet yetişkin, erkek Sprague Dawley sıçan 3 eşit gruba
ayrıldı (n=7). Kontrol grubuna hiçbir uygulama yapılmadı. DM grubundaki hayvanlara
tek doz 55 mg/kg streptozotosin intraperitoneal olarak verilerek DM
oluşturuldu. Mirtazapin grubuna streptozotosin uygulamasından 28 gün sonra, 14
gün boyunca günde 20 mg/kg mirtazapin gavaj yoluyla uygulandı. Deney sonunda
alınan beyincik örnekleri %10’luk formaldehit ile fikse edildikten sonra rutin doku
takip işleminin ardından alınan kesitlere hematoksilen ve eozin, krezil viyole boyama
teknikleri ve akson rejenerasyonunu belirlemek amacıyla da GAP-43
immünohistokimyasal boyaması uygulandı. Kontrol grubunda beyincikte normal histoloji
ile medüllada yüksek GAP-43 ekspresyonu gözlendi. DM grubunda Purkinje
nöronlarında büzüşme ve bazı alanlarda hücre ölümü saptanırken, orta düzeyde GAP-43
immünoreaksiyonu belirlendi. Mirtazapin uygulanan grupta ise Purkinje nöronlarının
ve histolojik yapının kontrol grubuna yakın olduğu gözlenirken GAP-43 ekspresyonunun
DM grubuna benzer olduğu saptandı. Sonuç olarak, streptozotosinle oluşturulan DM
sıçanların beyinciğinde özellikle Purkinje nöronlarında hasara ve medüllada
miyelin kaybına yol açmakta ve mirtazapin uygulaması Purkinje nöronlarındaki hasarı
azaltmakta, ancak miyelin rejenerasyonunu sağlamada belirgin bir etki göstermemektedir.

Anahtar Kelimeler

Diabetes mellitus, Serebellum, Mirtazapin, GAP43

Mirtazapine Partially Ameliorate Damage of the Diabetic Rat Cerebellum

Öz

Diabetes mellitus is a systemic
disease that causes damage to various organs. Among these organs, cerebellum is
also present. Mirtazapine is an antidepressant used in the treatment of major
depression also regulating the inflammatory response. With all this
information, we aimed to investigate the effects of DM on rat cerebellum and
effects of mirtazapine on DM-induced cerebellum damage. In our study 21 adult, male,
Sprague Dawley rats were equaly
divided into 3 groups (n = 7). No treatment was made to the control group.
Animals in the DM group received a single dose of 55 mg/kg streptozotocin
intraperitoneally. Mirtazapine was administered via gavage 20 mg / kg
mirtazapine daily for 14 days after 28 days of streptozotocin administration.
After 28 days of streptozotin administration 20 mg / kg mirtazapine was
administered to the mirtazapine group via gavage per day for 14 days. At the
end of the experiment, cerebellum specimens were fixed with 10% formaldehyde,
following routine tissue processing hematoxylin-eosin, krezil violet dyeing
techniques and GAP-43 immunohistochemical staining made to determine axonal
regeneration. The control group had high GAP-43 expression in medullary with
normal histology in the cerebellum. Moderate GAP-43 immunoreactivity, cell
death in some areas and shrinkage of Purkinje neurons was detected in the DM
group. In the mirtazapine group, Purkinje neurons were found to protect normal
structures relatively better, while GAP-43 expression was found to be similar
to the DM group. As a result, streptozotocin-induced DM leads to loss of myelin
in the brain, especially in Purkinje neurons damage, and mirtazapine
administration, which reduces damage in the Purkinje neurons, but does not have
a significant effect on myelin regeneration.

Anahtar Kelimeler

Diabetes mellitus, Cerebellum, Mirtazapine

Kaynakça

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