Erken Evre Kolon Kanserinde On Yıllık Nüks Sonuçları ve Bu Tümörlerde MikroRNA'ların ve Kanser Kök Hücre Belirteçlerinin Ekspresyon Profilleri

Öz

Amaç: MikroRNA'ların (miRNA'lar), kolon kanserinin erken evrelerinde (eCC) kanser kök hücre (CSC) belirteçlerini kritik olarak düzenleyerek tümör gelişiminde rol oynayabileceğini varsaydık. Bu çalışmanın amacı, CSC pozitif eCC hastalarında miRNA'ların ekspresyon profillerini belirlemek ve nüks ile ilişkilerini incelemektir. Materyal ve Metot: 30 eCC örneğinde CSC durumunu belirlemek için CD133, LGR5 ve SOX2 ekspresyon profillerini analiz ettik. Ardından, RT2 miRNA PCR özel dizilerinin sonuçlarını kullanarak, CSC pozitif eCC hastalarında 38 miRNA'nın ekspresyon profillerini değerlendirdik. Bulgular: Ameliyattan sonraki 10 yıl içinde 5 hastada nüks meydana geldi. CSC pozitif eCC hastalarında miR-125b'nin aşağı regülasyonunun ve miR-135b'nin yukarı regülasyonunun anlamlı olduğunu belirledik (sırasıyla p=0,21, p=0,001). miR-125b'nin düşük ifadesinin eCC'de tekrarlama ile ilişkili olduğunu bulduk (p=0,0022). Sonuç: eCC'de miR-125b ekspresyonunun arttırılmasıyla nüksün önlenebileceğini düşünüyoruz.

Anahtar Kelimeler

• kanser kök hücresi
• kolon kanseri
• erken başlangıçlı
• erken evre
• mikroRNA

Ten-Year Recurrence Outcomes in Early Stage and Early Onset Colon Cancer and the Expression Profiles of MicroRNAs and Cancer Stem Cell Markers in These Tumors

Abstract

Objective: We hypothesized that microRNAs (miRNAs) might be involved in tumor development by critically regulating cancer stem cell (CSC) markers in the early stages of colon cancer (eCC). This study aimed to determine the expression profiles of miRNAs in CSC-positive eCC patients and examine their associations with recurrence. Materials and Methods: We analyzed CD133, LGR5 and SOX2 expression profiles to determine CSC status in 30 eCC specimens. Then, using the results of RT2 miRNA PCR custom arrays, we evaluated the expression profiles of 38 miRNAs in CSC-positive eCC patients. Results: Recurrence occurred in 5 patients within ten years after surgery. We determined down-regulation of miR-125b and up-regulation of miR-135b were significant in CSC-positive eCC patients (p=0.021, p=0.001, respectively). We found that low expression of miR-125b was associated with recurrence in eCC (p=0.0022). Conclusions: We suggest that recurrence might be prevented by increasing the expression of miR-125b in eCC.

Keywords

• cancer stem cell
• colon cancer
• early-onset
• early-stage
• microRNA

Kaynakça

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