Prostat Kanserinde Yağ Damlacık Oluşumu ile İlişkili PLA2G7, UCP2 ve NEDD4L Genlerinin Protein Ekspresyonlarının Araştırılması

Öz

Amaç: Prostat kanseri (PCa) düzensiz lipid metabolizması ile karakterize edilen bir kanserdir. PCa hücrelerinin; lipitleri lipid damlacıkları şeklinde depoladığı ayrıca proliferasyon ve büyümeyi kolaylaştırmak amacıyla membran sentezi için yapıtaşı olarak kullandığı gözlemlenmiştir. Bu çalışmada amacımız PC3 ve DU145 prostat kanseri hücre hatlarında lipid damlacık metabolizmasında görev alan PLA2G7, UCP2 ve NEDD4L proteinlerinin ekspresyon seviyelerini incelemektir. Materyal ve Metot: Prostat kanseri hücre hatları orta seviye metastatik DU145 ve ileri metastatik PC3 %10 FBS, %1 penisilin-streptomisin, %1 non-esansiyel aminoasit içeren yüksek glukoz DMEM besi yerinde çoğaltılmıştır. Daha sonra PLA2G7, UCP2 ve NEDD4L proteinlerinin ekspresyon düzeyleri ELISA testi ile incelenmiştir. Bulgular: Bu çalışmada, PC3 ve DU145 hücrelerinde PLA2G7 protein seviyeleri açısından anlamlı bir fark olmadığı (p>0,05) ancak UCP2 protein seviyelerinin PC3 hücrelerinde anlamlı derecede arttığı gösterilmiştir (p<0,05). Bunun aksine NEDD4L protein seviyeleri DU145 hücre hattında anlamlı derecede düşmüştür (p<0,05). Sonuç: Çalışmanın sonucunda, UCP2'nin prostat kanserinin ilerlemesinde rolü ve NEDD4L ile hücre proliferasyonu kontrolü ile arasında bir ilişki olabilir.

Anahtar Kelimeler

• Lipid damlacığı
• NEDD4L
• PLA2G7
• Prostat kanseri
• UCP2

Investigation of Protein Expressions of PLA2G7, UCP2 and NEDD4L Genes Associated with Fat Droplet Formation in Prostate Cancer

Öz

Objective: Prostate cancer (PCa) is characterised by dysregulated lipid metabolism. PCa cells store lipids as lipid droplets and use them to facilitate proliferation and growth. In this study, we aim to investigate the expression levels of PLA2G7, UCP2 and NEDD4L proteins, which are involved in lipid droplet metabolism, in PC3 (advanced metastatic) and DU145 (intermediate metastatic) PCa cells. Materials and Methods: DU145 and PC3 cells were cultured in a high-glucose DMEM medium containing 10% FBS, 1% penicillin-streptomycin, and 1% non-essential amino acid. The expression levels of PLA2G7, UCP2, and NEDD4L protein were assessed with ELISA assay. Results: There was no significant difference in the protein level of PLA2G7 between the PC3 and DU145 cells (p>0.05), while the protein level of UCP2 increased in the PC3 cell line significantly (p<0.05). The protein level of NEDD4L decreased significantly in the DU145 cell line when compared to the PC3 cell line (p<0.05). Conclusions: As a result of this study, the UCP2 gene might play a role in the progression of prostate cancer, and there could be a relationship between NEDD4L and cell proliferation control.

Anahtar Kelimeler

• Lipid droplet
• NEDD4L
• PLA2G7
• Prostate cancer
• UCP2

Kaynakça

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