Enzymes During Pregnancy: Cytosolic Carbonic Anhydrase, Catalase, Paraoxonase 1 and Xanthine Oxidase Levels

Yıl 2019, , 79 - 93, 31.03.2019

Ender Şimşek , Ayşe Filiz Yavuz Emine Terzi Tuğba Kevser Uysal Beyza Ecem Öz Bedir Emin Haqverdiyev Özen Özensoy Güler

https://doi.org/10.26453/otjhs.409112

Cited By: 2

Öz

Pregnancy is a physiological condition
which requires high energy and therefore more oxygen for many body functions.
This increase in oxygen and energy requirements leads to an increase in
oxidative stress. High energy and increased oxygen are needed in the duration
of pregnancy - 1st, 2nd and 3rd trimesters. There can be significant
correlations between biochemical changes and some enzyme activities. This study
investigates the activities of cytosolic CA, CAT, PON 1 and XO enzymes during
pregnancy. The antioxidant enzymes’ activities were measured
spectrophotometrically using the UV assay method on a Biotek. Cytosolic CA
ctivity was measured by the hydration of CO2 in accordance with Wilbur and
Anderson's method. According to the findings of this study, CAT (p=0.048) and
cytosolic CA (p<0.001) activities decreased during pregnancy whereas both
PON 1 (p<0.001) and XO (p=0.016) activity levels were higher in pregnancy.
However, there were no statistically significant difference between 1st, 2nd
and 3rd trimesters of pregnancy in terms of cytosolic CA, CAT, PON 1 or XO enzyme
levels (p>0.05).Different antioxidant enzymes' activities may increase or
decrease during pregnancy. The decrease of CA enzyme levels in the group
consisting pregnants may have significant impact on biochemical and
physiological aspect of fetal and maternal health.

Anahtar Kelimeler

Pregnancy, catalase, paraoxanase, xanthine oxidase, cytosolic carbonic anhydrase

Gebelikte Enzimler: Sitozolik Karbonik Anhidraz, Katalaz, Paraoksonaz 1 ve Ksantin Oksidaz Düzeyleri

Öz

Gebelik, birçok vücut fonksiyonu için
yüksek enerji ve dolayısıyla daha fazla oksijen gerektiren fizyolojik bir
durumdur. Oksijen ve enerji gereksinimlerindeki bu artış, oksidatif streste
artışa neden olmaktadır. Gebeliğin 1., 2. ve 3. trimesterlarında yüksek enerji
ve artmış oksijen ihtiyacı duyulur. Bu biyokimyasal değişiklikler ile bazı
enzim aktiviteleri arasında anlamlı ilişki olabilir. Yapılan bu çalışmada
gebelik sırasında sitozolik CA, CAT, PON 1 ve XO enzim aktiviteleri
araştırılmıştır. Antioksidan enzimlerin aktiviteleri Biotek cihazı ile
spektrofotometrik olarak ölçülmüştür. Sitozolik CA aktivitesi, Wilbur ve
Anderson yöntemine göre CO2'nin hidratasyonu ile ölçülmüştür. Bulgulara göre,
gebelikte CAT (p=0,048) ve sitozolik CA (p<0,001) enzim aktiviteleri azalırken,
PON 1 (p<0,001) ve XO (p=0,016) enzim aktiviteleri artmıştır. Bununla
birlikte, sitozolik CA, CAT, PON 1 veya XO enzim düzeyleri açısından 1., 2. ve
3. trimesterlar arasında istatistiksel olarak anlamlı bir fark saptanamamıştır
(p>0,05). Farklı antioksidan enzim aktiviteleri hamilelikte artabilir veya
azalabilir. Ayrıca, gebe grubunda sitozolik CA enzim düzeyleri azalmasının
fetal ve maternal sağlığın biyokimyasal ve fizyolojik yönleri üzerinde önemli
etkisi olabilir.

Anahtar Kelimeler

Gebelik, katalaz, paraoksonaz, ksantin oksidaz, sitozolik karbonik anhidraz

Kaynakça

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