Anjiyografi Hasta Plazmalarında, Paraoxonase 1, Arylesterase ve Total Thiol İncelemesi

Yıl 2022, , 8 - 12, 01.03.2022

Hayrullah Yazar , Güler Kuşçu Günay , Mustafa Baran İnci , Süleyman Kaleli , Mehmet Çakar

https://doi.org/10.26453/otjhs.905082

Öz

Amaç: Radyal koroner anjiyografi uygulanan erişkin hastalarda; Paraoxonase-1 (PON 1), Arilesteraz (ARE) ve Total tiyol (TTL) değerlerinin, kan plazmasında tespit edilmesi amaçlandı.
Materyal ve Metot: Çalışmanın katılımcı sayısı, birisi kontrol diğeri hasta grubu olmak üzere, iki grupta toplam 68 oldu. Kontrol grubu 34 sağlıklı bireyden oluşurken, hasta gurubu ise 34 radyal koroner anjiyografi hastasından oluştu. Kan numuneleri lityum heparinli tüplere alındıktan sonra laboratuvara transfer edildi ve burada santrifüj işleminden sonra ayrılan plazmaları, çalışma gününe kadar kapaklı ependorf tüplerde -80C0 de saklandı. Test parametreleri tam otomatik analizörde spektrofotometrik metot ile incelendi. Çalışmada elde edilen veriler SPSS 23 paket programı ile istatistiksel olarak analiz edildi. Anlamlılık düzeyi, p<0,.05 olarak kabul edildi.
Bulgular: Hasta grubundaki ortalama TTL değerleri (360,29 μmol/L) kontrol grubuna göre (482,25 μmol/L) anlamlı derecede düşük bulundu (p<0,001). Benzer şekilde hastaların ortalama ARE değerleri (11,30 U/L) kontrol grubuna göre (14,28 U/L) anlamlı derecede düşük bulundu (p<0,001). Hasta grubu PON 1 (U/L) değerleri sırasıyla (medyan, Q1, Q3): 140,78; 100,06; 322,12 olarak bulundu. Kontrol grubu PON 1 (U/L) değerleri ise, sırasıyla: 153,65; 104,9Q8; 454,13 şeklinde bulundu. Bu değerler arasında anlamlı bir fark olmadığı görüldü (p=0,30).
Sonuç: Anjiyografi hastaları plazma değerleri, lipofilik antioksidan özelliklerde azalma olduğunu gösterdi. PON 1 değerleri, istatistiksel olarak anlamlı olmamasına rağmen bu görüşü destekledi. Öte yandan; hasta grubunda tespit edilen anlamlı TTL düşüklüğü, üzerinde daha geniş araştırmalar gerektiren bir sonuç olarak tespit edildi. 

Anahtar Kelimeler

Arylesterase, paraoxonase 1, renal anjiyografi, total tiyol

Destekleyen Kurum

BAP Hızlı Destek

Proje Numarası

12017-08-06-003

Paraoxonase1, Arylesterase and Total Thiol Investigation in Angiography Patient Plasmas

Öz

Objective: In adult patients undergoing radial coronary angiography; It was aimed to determine the values ​​of paraoxonase-1, Arylesterase and Total thiol in blood plasma.
Materials and Methods: The number of participants in the study was 68 in two groups, one for the control group and the other for the patient group. While the control group consisted of 34 healthy individuals, the patient group consisted of 34 radial coronary angiography patients. After blood samples were taken into lithium heparin tubes, they were transferred to the laboratory and the plasma separated after centrifugation was stored at -80 °C in capped eppendorf tubes until the working day. Test parameters were analyzed by spectrophotometry method on a fully automated analyzer. The data obtained in the study were statistically analyzed with the SPSS 23 package program. Significance level was accepted as p<0.05.
Results: The mean TTL values ​​in the patient group (360.29 μmol/L) were found to be significantly lower than the control group (482.25 μmol/L) (p <0.001). Similarly, the mean ARE values ​​of the patients (11.30 U/L) were found to be significantly lower than the control group (14.28 U/L) (p<0.001). Patient group PON 1 (U/L) values, respectively (median, Q1, Q3): 140.78; 100.06; 322.12 were found. Control group PON 1 (U/L) values: 153.65; 104.9Q8; 454.13 were found. There was no significant difference between these values (p=0.30).
Conclusion: Angiography patients showed a decrease in plasma values, lipophilic antioxidant properties. PON 1 values supported this view, although it was not statistically significant. On the other hand; Significant low TTL detected in the patient group was determined as a result that requires further studies. 

Anahtar Kelimeler

Arylesterase, paraoxonase 1, renal angiography, total thiol

Proje Numarası

12017-08-06-003

Kaynakça

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