Mikrodalga ön işleminden geçirilmiş atık kuru incirin biyohidrojen üretimi için değerlendirilmesi

Öz

Çalışmada; atık kuru incirde mikrodalga ön işlemi (250-800W) uygulanarak aflotoksin giderimi ve en yüksek çözünmüş şeker eldesi için en iyi koşulları saptanmıştır. Aflatoksini giderilmiş atık kuru incirlerin biyohidrojen üretim performansları farklı substrat (11.8-118 g/L) ve farklı organizma konsantrasyonları (0.5-2.5 g/L) denenerek test edilmiştir. Mikrodalga işlemi sonucunda 100 g/L atık kuru incirden en yüksek çözünmüş şeker konsantrasyonunu (89.7 g/L) veren dalga boyu 400 W ve muamele süresi 10 dk. olarak bulunmuştur. Bu koşullarda %46.2 şeker hidrolizlenmesi ve %77.8 aflatoksin giderimi sağlanmıştır. En yüksek kümülatif hidrojen gazı hacmi, hidrojen gazı üretim verimi, hidrojen gazı üretim hızı; 59 g/L başlangıç substrat konsantrasyonunda sırasıyla, 161.9 mL, 207.5 mL H2/g toplam şeker, 1.75 mL/sa. olarak bulunmuştur. Farklı substrat konsantrasyonlarının kullanıldığı deneylerde üretilen organik asitlerden, laktik asidin yüksek miktarlarda üretiliyor olmasına rağmen, bazı deneylerde bütrik aside dönüştüğü gözlemlenmiştir. Bu da biyohidrojen gazı üretimlerinde artış sağlamıştır. Organizma miktarı değişim deneylerinde şekerin tüm deney ortamlarında tüketildiği gözlenmiş ve başarılı bir şekilde hidrojen gazı üretimi gerçekleşmiştir. Yoğunluklu olarak bütrik asit üretilen deney ortamlarında en yüksek hidrojen gazı üretim verimi 1 g/L organizma içeren deney ortamında 124.28 mL H2/g toplam şeker olarak elde edilmiştir. Organizma konsantrasyonu arttıkça spesifik hidrojen üretim hızında (SHÜH) düşüş gözlenmiştir. En yüksek SHÜH, 0.5 g/L organizma içeren fermentasyon ortamında 3.83 mL H2/g biyokütle saat olarak bulunmuştur.

Anahtar Kelimeler

• Aflatoksin
• Atık kuru incir
• Biyohidrojen üretimi
• Substrat konsantrasyonu
• Organizma konsantrasyonu

Evaluation of microwave pretreated waste dry fig for Biohydrogen production

Abstract

In the study, the best conditions for aflatoxin removal and the highest dissolved sugar were determined by applying microwave pretreatment (250-800W) to waste dried figs. Biohydrogen production performances of aflatoxin-free dried figs were evaluated by testing different substrate (11.8-118 g/L) and different organism concentrations (0.5-2.5 g/L). The optimum microwave of the microwave pretreatment process, giving the highest dissolved sugar concentration (89.7 g/L) from 100 g/L waste dried figs was 400 W and the treatment time was 10 minutes. Under these conditions, 46.2% hydrolysis of sugars and 77.8% aflatoxin removal were achieved. At the initial substrate concentration of 59 g/L, the highest cumulative volume of hydrogen gas, hydrogen gas production yield, hydrogen gas production rate were found as 161.9 mL, 207.5 mL H2/g total sugar, 1.75 mL/hour, respectively. Although lactic acid was produced in high amounts from organic acids produced in experiments using different substrate concentrations, it has been observed that it is converted into butyric acid in some experiments. This resulted as an increase in biohydrogen gas production. In different organism amount experiments, it was observed that sugar was consumed in all experiments and hydrogen gas was produced successfully. Butyric acid was the main organic acid in the experimental media where the highest hydrogen gas production yield was produced in 1 g/L as 124.28 mL H2/g total sugar. The specific hydrogen gas production rate (SHPR) decreased with increasing organism concentrations. The highest SHPR was obtained with 0.5 g/L organism concentration as 3.83 mL H2/g biomass hour.

Keywords

• Aflatoxin
• Waste dry fig
• Biohydrogen production
• Substrate concentration
• Organism concentration

Kaynakça

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