Elektro üretim tekniği kullanılarak postbiyotik içeren nanoliflerin üretimi

Öz

Postbiyotikler, çeşitli yöntemler ile üretilen ve canlı hücre içermeyen metabolitlerdir. İçerdikleri biyoaktif ajanlar ile bulundukları ortamda antimikrobiyal, antiviral ve antienflamatuar gibi çeşitli fizyolojik aktiviteleri teşvik ederler. Canlı hücre içermedikleri için antibiyotik direnci oluşturmazlar, bağışıklığı düşük bireylerde kullanımı güvenlidir ve soğuk zincir gerektirmeyen taşıma ve depolama kolaylıkları ile probiyotiklere kıyasla birçok üstün özelliklere sahiptir. Nanolifler geniş yüzey alanı ve yüksek gözenekli yapıları ile birçok kullanım alanına sahiptir. Nanoliflerin üretiminde en sık kullanılan yöntemlerden biri elektro üretimdir. Bu çalışma kapsamında elektro üretim tekniği ile üretilen polimer nanolif örtülerin yapılarına Lactobacillus acidophilus ve Lactobacillus rhamnosus suşlarından elde edilen postbiyotiklerin dahil edilebilirliği ve nanoliflerin antimikrobiyal aktivitesi analiz edilmiştir. Yapılan analizlerden elde edilen bulgularda postbiyotiklerin nanolif örtülere dahil edilebildiği ve bununla birlikte 5 farklı patojen mikroorganizmaya karşı iyi bir antimikrobiyal etki sağladığı ortaya konmuştur.

Anahtar Kelimeler

• Postbiyotik
• Probiyotik
• Nanolif
• Antimikrobiyal Elektro üretim

Production of nanofibers containing postbiotics using electrospinning method

Öz

Postbiotics are metabolites produced by various methods that do not contain live cells. These metabolites promote various physiological activities, such as antimicrobial, antiviral, and anti-inflammatory effects, through the bioactive agents they contain. Since they do not contain live cells, they do not contribute to antibiotic resistance, making their use safe for individuals with compromised immune systems. Moreover, they offer several advantages over probiotics, such as not requiring a cold chain for transport and storage. Nanofibers, known for their large surface area and highly porous structure, have a wide range of applications. One of the most commonly used methods for producing nanofibers is the electrospinning technique. In this study, the incorporation of postbiotics derived from Lactobacillus acidophilus and Lactobacillus rhamnosus strains into polymer nanofiber mats produced by electrospinning was investigated, along with the antimicrobial activity of these nanofibers. The findings from the analyses demonstrated that postbiotics could be successfully incorporated into nanofiber mats and provided significant antimicrobial effects against five different pathogenic microorganisms

Anahtar Kelimeler

• Postbiotic
• Probiotic
• Nanofiber
• Antimicrobial Electrospinning

Kaynakça

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