Changes in microbial structure in the anammox population at high-temperature

Yıl 2025, Cilt: 31 Sayı: 6, 1060 - 1072, 13.11.2025

Tuğba Sarı

https://doi.org/10.5505/pajes.2025.69449

https://izlik.org/JA53JG23PE

Öz

Anammox bacteria are crucial in converting ammonia nitrogen into N2 gas in anoxic conditions, offering sustainable alternatives to biological wastewater treatment. However, temperature fluctuations in wastewater treatment plants significantly impact the activity of microorganisms, including anammox bacteria. This underscores the importance of understanding the influence of temperature on microbial ecology. Although extensive research has been conducted on anammox activity across various temperatures, the effects of high temperatures on microbial diversity within anammox consortia remain largely unexplored. This study aims to investigate the effects of high temperatures on microbial performance and shifts in microbial diversity in anammox bioreactors. Two separate bioreactors were employed to validate the findings. After enriching anammox cultures under mesophilic conditions, the bioreactors were exposed to 65°C for 9 hours, followed by a recovery phase at 35°C. Thermal stress led to a sharp decline in nitrogen removal efficiency, with NH4+-N and NO2--N removal rates decreasing by up to 83.59% and 91.35%, respectively. Even after reducing influent concentrations, nitrogen removal performance remained impaired. Metagenomic analyses revealed substantial shifts in microbial composition at elevated temperatures. While phyla such as Chloroflexi and Bacteroidota diminished, Proteobacteria and Firmicutes proliferated. Notably, Candidatus Kuenenia (anammox genus) declined, indicating its sensitivity to thermal stress. These findings provide valuable insights into how high temperatures influence microbial diversity in anammox systems. Future research should focus on improving microbial resilience and stability to optimize anammox system performance in harsh environmental conditions.

Anahtar Kelimeler

Anammox , Wastewater treatment , High temperature , Metagenomics , Microbial composition

Yüksek sıcaklıkta anammox popülasyonunda mikrobiyal yapıdaki değişiklikler

https://izlik.org/JA53JG23PE

Öz

Anammox bakterileri, anoksik koşullar altında amonyak azotunu N2 gazına dönüştürmede kritik öneme sahiptir ve biyolojik atık su arıtımına sürdürülebilir alternatifler sunar. Ancak, atık su arıtma tesislerindeki sıcaklık dalgalanmaları, anammox bakterileri de dahil olmak üzere mikroorganizmaların aktivitelerini önemli ölçüde etkilemektedir. Bu durum, sıcaklığın mikrobiyal ekoloji üzerindeki etkisini anlamanın önemini ortaya koymaktadır. Çeşitli sıcaklıklarda anammox aktivitesi üzerine kapsamlı araştırmalar yürütülmüş olmasına rağmen, yüksek sıcaklıkların anammox konsorsiyumlarındaki mikrobiyal çeşitlilik üzerindeki etkileri büyük ölçüde keşfedilmemiştir. Bu çalışma, anammox biyoreaktörlerinde yüksek sıcaklıkların mikrobiyal performans üzerindeki etkilerini ve mikrobiyal çeşitlilikteki değişimleri araştırmayı amaçlamaktadır. Bulguları doğrulamak için iki ayrı biyoreaktör kullanılmıştır. Anammox kültürleri mezofilik koşullar altında zenginleştirildikten sonra, biyoreaktörler 9 saat boyunca 65 °C’ye maruz bırakılmış ve ardından 35 °C’de bir geri kazanım sürecine tabi tutulmuştur. Termal stres, azot giderim verimliliğinde keskin bir düşüşe neden olmuş; NH4+-N ve NO2--N giderim oranları sırasıyla %83,59 ve %91,35'e kadar azalmıştır. Giriş konsantrasyonlarının azaltılmasına rağmen, azot giderim performansı iyileşme göstermemiştir. Metagenomik analizler, yüksek sıcaklıklarda mikrobiyal bileşimde önemli değişiklikler olduğunu ortaya koymuştur. Chloroflexi ve Bacteroidota gibi filumlar azalırken, Proteobakteriler ve Firmicutes artmıştır. Özellikle, Candidatus Kuenenia (anammox türü) bir düşüş göstermiştir ve bu durum bu türün termal strese duyarlılığını ortaya koymaktadır. Bu bulgular, yüksek sıcaklıkların anammox sistemlerindeki mikrobiyal çeşitlilik üzerindeki etkilerine ilişkin değerli bilgiler sunmaktadır. Gelecekteki araştırmalar, zorlu çevre koşullarında anammox sistemlerinin performansını optimize etmek için mikrobiyal dayanıklılığı ve stabiliteyi artırmaya odaklanmalıdır.

Anahtar Kelimeler

Anammox , Atık su arıtımı , Yüksek sıcaklık , Metagenomik , Mikrobiyal kompozisyon

Kaynakça

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