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Calcium carbonate precipitation by urease and carbonic anhydrase positive bacteria

Yıl 2020, Cilt: 26 Sayı: 3, 513 - 518, 08.06.2020

Öz

In present study, CaCO3 precipitation was examined in two ureolytic bacteria. Bacillus aerius U2 and Sporosarcina pasteurii ATCC 6453 were used as test organisms. The determination of urease and carbonic anhydrase enzyme activities were also determined. For further confirmation of the calcium carbonate mineral type produced by bacteria, XRD, SEM and EDX analysis were done. Strain U2 produced calcite and vaterite. In S. pasteurii ATCC 6453, only vaterite was found. The enzyme activity studies showed that both urease and carbonic anhydrase activities was 2-50-fold higher in S. pasteurii ATCC 6453 than B. aerius U2. Although, S. pasteurii ATCC 6453 was better option for microbial calcium carbonate precipitation (MCP) at higher temperature, by B. aerius U2 at lower temperature (<30 °C) is made possible to employ in the most geotechnical applications.

Kaynakça

  • Henze J, Randall DG. “Microbial induced calcium carbonate precipitation at elevated pH values (>11) using Sporosarcina pasteurii”. Journal of Environmental Chemical Engineering, 6(4), 5008-5013, 2018.
  • Canakci H, Sidik W, Kilic IH. “Effect of bacterial calcium carbonate precipitation on compressibility and shear strength of organic soil”. Soils and Foundations, 55(5), 1211-1221, 2015.
  • Bundur ZB, Amiri A, Ersan YC, Boon N, Belie ND. “Impact of air entraining admixtures on biogenic calcium carbonate precipitation and bacterial viability”. Cement and Concrete Research, 98, 44-49, 2017.
  • Ferris FG, Phoenix V, Fujita Y, Smith RW. “Kinetics of calcite precipitation induced by ureolytic bacteria at 10 to 20 oC in artificial groundwater”. Geochimica et Cosmochimica Acta, 68 (8), 1701-1710, 2004.
  • Chu J, Stabnikov V, Ivanov V. “Microbially induced calcium carbonate precipitation on surface or in the bulk of soil”. Geomicrobiology Journal, 29 (6), 544-549, 2012.
  • Amiri A, Azima M, Bundur ZB. “Crack remediation in mortar via biomineralization: Effects of chemical admixtures on biogenic calcium carbonate”. Construction and Building Materials, 190, 317-325, 2018.
  • Ksara M, Newkirk R, Langroodi SK, Althoey F, Sales , Schauer CL, Farnam Y. “Microbial damage mitigation strategy in cementitious materials exposed to calcium chloride”. Construction and Building Materials, 195, 1-9, 2019.
  • Qabany A, Soga K, Santamarina C. “Factors affecting efficiency of microbially induced calcite precipitation”. Journal of Geotechnical and Geoenvironmental Engineering, 138 (8), 992-1001, 2012.
  • Li Q, Zhang B, Ge Q, Yang X. “Calcium carbonate precipitation induced by calcifying bacteria in culture experiments: Influence of the medium on morphology and mineralogy”. International Biodeterioration & Biodegradation, 134, 83-92, 2018.
  • Zhu T, Lin Y, Lub X, Dittrich M. “Assessment of cyanobacterial species for carbonate precipitation on mortar surface under different conditions”. Ecological Engineering, 120, 154-163, 2018.
  • Schwantes-Cezario N, Medeiros LP, De Oliveira Jr. AG, Nakazato G, Kobayashi RKT, Toralles BM. “Bioprecipitation of calcium carbonate induced by Bacillus subtilis isolated in Brazil”. International Biodeterioration and Biodegradation, 123, 200-205, 2017.
  • Zhuang D, Yan H, Tucker ME, Zhao H, Han Z, Zhao Y, Sun B, Li D, Pan J, Zhao Y, Meng R, Shan G, Zhang X, Tang R. “Calcite precipitation induced by Bacillus cereus MRR2 cultured at different Ca2+ concentrations: Further insights into biotic and abiotic calcite”. Chemical Geology, 500, 64-87, 2018.
  • Bang SS, Galimat JK, Ramakrishan V. “Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii”. Enzyme and Microbial Technology, 28(4-5), 404-409, 2001.
  • Rodriguez-Navarro C, Rodriguez-Gallego M, Chekroun KB, Muñoz MT. “Conservation of ornamental stone by Myxococcus xanthus-induced carbonate biomineralization”. Applied and Environmental Microbiology, 69(4), 2182-2193, 2003.
  • Lee NY. “Calcite production by Bacillus amyloliquefaciens CMB01”. Journal of Microbiology, 41(4), 345-348, 2003.
  • Chahal N, Rajor A, Siddique R. “Calcium carbonate precipitation by different bacterial strains”. African Journal of Biotechnology, 10(2), 8359-8372, 2011.
  • Akyol E, Bozkaya O, Dogan NM. “Strengthening sandy soils by microbial methods”. Arabian Journal of Geosciences, 10(327), 1-8, 2017.
  • American Public Health Association (APHA). “Standard methods for the Examination of Water and Wastewater”. Washington, USA, 17th ed.,1989.
  • Weatherburn MW. “Phenol-hypochlorite reaction for determination of ammonia”. Analytical Chemistry, 39(8), 971-974, 1967.
  • Armstrong JM, Myers DV, Verpoorte JA, Edsall JT. “Purification and properties of human erythrocyte carbonic anhydrases”. Journal of Biological Chemistry, 241(21), 5137-5149, 1966.
  • Sun X, Miao L, Tong T, Wang C. “Study of the effect of temperature on microbially induced carbonate precipitation”. Acta Geotechnica, 14(3), 627-638, 2019.
  • Omoregie AI, Ong DEL, Nissom PM. “Assessing ureolytic bacteria with calcifying abilities isolated from limestone caves for biocalcification”. Letters in Applied Microbiology, 68 (2), 173-181, 2018.
  • Mitchell AC, Ferris FG. “The Ca precipitation into calcite precipitates induced by bacterial ureolysis in artificial ground water: temperature and kinetics dependence”. Geochimica Cosmochimica Acta, 69, 4199-4210, 2005.
  • Rodriguez-Navarro C, Jimenez-Lopez C, Rodriguez-Navarro A, Gonzalez-Munöz MT, Rodriguez-Gallego M. “Bacterially mediated mineralization of vaterite”. Geochimica Cosmochimica Acta, 71 (5), 1197-1213, 2007.
  • Stocks-Fischer S, Galinate JK, Bang SS. “Microbiological precipitation of CaCO3”. Soil Biology and Biochemistry, 31 (11), 1563-1571, 1999.
  • Anne S, Rozen Bamu O, Andreazza P, Rouet JL. “Evidence of a bacterial carbonate coating on plaster samples subjected to the calcite bioconcept biomineralization techniques”. Construction and Building Materials, 24 (6), 1036-1042, 2010.
  • Prah J, Maˇcek J, Draˇzic G. “Precipitation of calcium carbonate from a calcium acetate and ammonium carbamate batch system”. Journal of Crystal Growth, 324 (1), 229-234, 2011.
  • Hammes F, Boon N, de Villiers J, Verstraete W, Siciliano SD. “Strain-Specific ureolytic microbial calcium carbonate precipitation”. Applied and Environmental Microbiology, 69(8), 4901-4909, 2003.
  • Li W, Chen WS, Zhou PP, Cao L, Yu LJ. “Influence of initial pH on the precipitation and crystal morphology of calcium carbonate induced by microbial carbonic anhydrase”. Colloids and Surfaces B: Biointerfaces, 102, 281-287, 2013.
  • Chunxiang Q, Jianyun W, Ruixing W, Liang C. “Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii”. Materials Science and Engineering C, 29(4), 1273-1280, 2009.
  • Obst M, Dynes JJ, Lawrence JR, Swerhone GDW, Benzerara K., Karunakaran C, Kaznatcheev K, Tyliszczak T, Hitchcock AP. “Precipitation of amorphous CaCO3 (aragonite-like) by cyanobacteria: A STXM study of the influence of EPS on the nucleation process”. Geochimica et Cosmochimica Acta, 73 (14), 4180-4198, 2009.
  • Kaur DN, Reddy SM, Mukherjee A. “Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites”. Journal of Microbiology and Biotechnology, 23(5), 707-714, 2013.
  • Priya JN, Kannan M. “Effect of carbonic anhydrase and urease on bacterial calcium carbonate precipitation”. Int. Journal of Pharma and Bio Science, 8(3), 609-614, 2017.
  • Sensoy T, Bozbeyoglu NN, Dogan NM, Bozkaya O, Akyol E. “Characterization of Calcium Carbonate Produced by ureolytic bacteria (Sporocarcina pasteurii ATCC 6453 and Bacillus aerius U2) and Effect of Environmental Conditions on Production of Calcium Carbonate”. https://cest.gnest.org/sites/default/files/presentation_file_list/cest2017_00840_poster_paper.pdf. (19.04. 2017).

Üreaz ve karbonik anhidraz pozitif bakteriler tarafindan kalsiyum karbonat çökelimi

Yıl 2020, Cilt: 26 Sayı: 3, 513 - 518, 08.06.2020

Öz

Bu çalışmada, iki üreolitik bakteride CaCO3 çökelimi araştırılmıştır. Test organizması olarak Bacillus aerius U2 ve Sporosarcina pasteurii ATCC 6453 türleri kullanılmıştır. Bunların yanında, bakterilerin üreaz ve karbonik anhidraz enzim aktiviteleri de tespit edilmiştir. Bakteriler tarafından üretilen kalsiyum karbonatın mineral tipi konformasyonu için XRD, SEM ve EDX analizleri gerçekleştirilmiştir. B. aerius U2 suşu kalsit ve vaterit üretmiştir. S. pasteurii ATCC 6453 suşunda sadece vaterit bulunmuştur. Enzim aktivitesi çalışmalarının sonuçlarına göre S. pasteurii ATCC 6453, B. aerius U2’den 2-50 kat daha fazla aktivite göstermiştir. Yüksek sıcaklıklarda kalsiyum karbonat çökelimi için S. pasteurii ATCC 6453 türü tercih edilebilirken, daha geniş iklim bölgelerini kapsayan düşük sıcaklıklarda(<30 °C) B. aerius U2 türünün kullanım potansiyeli bulunmaktadır.

Kaynakça

  • Henze J, Randall DG. “Microbial induced calcium carbonate precipitation at elevated pH values (>11) using Sporosarcina pasteurii”. Journal of Environmental Chemical Engineering, 6(4), 5008-5013, 2018.
  • Canakci H, Sidik W, Kilic IH. “Effect of bacterial calcium carbonate precipitation on compressibility and shear strength of organic soil”. Soils and Foundations, 55(5), 1211-1221, 2015.
  • Bundur ZB, Amiri A, Ersan YC, Boon N, Belie ND. “Impact of air entraining admixtures on biogenic calcium carbonate precipitation and bacterial viability”. Cement and Concrete Research, 98, 44-49, 2017.
  • Ferris FG, Phoenix V, Fujita Y, Smith RW. “Kinetics of calcite precipitation induced by ureolytic bacteria at 10 to 20 oC in artificial groundwater”. Geochimica et Cosmochimica Acta, 68 (8), 1701-1710, 2004.
  • Chu J, Stabnikov V, Ivanov V. “Microbially induced calcium carbonate precipitation on surface or in the bulk of soil”. Geomicrobiology Journal, 29 (6), 544-549, 2012.
  • Amiri A, Azima M, Bundur ZB. “Crack remediation in mortar via biomineralization: Effects of chemical admixtures on biogenic calcium carbonate”. Construction and Building Materials, 190, 317-325, 2018.
  • Ksara M, Newkirk R, Langroodi SK, Althoey F, Sales , Schauer CL, Farnam Y. “Microbial damage mitigation strategy in cementitious materials exposed to calcium chloride”. Construction and Building Materials, 195, 1-9, 2019.
  • Qabany A, Soga K, Santamarina C. “Factors affecting efficiency of microbially induced calcite precipitation”. Journal of Geotechnical and Geoenvironmental Engineering, 138 (8), 992-1001, 2012.
  • Li Q, Zhang B, Ge Q, Yang X. “Calcium carbonate precipitation induced by calcifying bacteria in culture experiments: Influence of the medium on morphology and mineralogy”. International Biodeterioration & Biodegradation, 134, 83-92, 2018.
  • Zhu T, Lin Y, Lub X, Dittrich M. “Assessment of cyanobacterial species for carbonate precipitation on mortar surface under different conditions”. Ecological Engineering, 120, 154-163, 2018.
  • Schwantes-Cezario N, Medeiros LP, De Oliveira Jr. AG, Nakazato G, Kobayashi RKT, Toralles BM. “Bioprecipitation of calcium carbonate induced by Bacillus subtilis isolated in Brazil”. International Biodeterioration and Biodegradation, 123, 200-205, 2017.
  • Zhuang D, Yan H, Tucker ME, Zhao H, Han Z, Zhao Y, Sun B, Li D, Pan J, Zhao Y, Meng R, Shan G, Zhang X, Tang R. “Calcite precipitation induced by Bacillus cereus MRR2 cultured at different Ca2+ concentrations: Further insights into biotic and abiotic calcite”. Chemical Geology, 500, 64-87, 2018.
  • Bang SS, Galimat JK, Ramakrishan V. “Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii”. Enzyme and Microbial Technology, 28(4-5), 404-409, 2001.
  • Rodriguez-Navarro C, Rodriguez-Gallego M, Chekroun KB, Muñoz MT. “Conservation of ornamental stone by Myxococcus xanthus-induced carbonate biomineralization”. Applied and Environmental Microbiology, 69(4), 2182-2193, 2003.
  • Lee NY. “Calcite production by Bacillus amyloliquefaciens CMB01”. Journal of Microbiology, 41(4), 345-348, 2003.
  • Chahal N, Rajor A, Siddique R. “Calcium carbonate precipitation by different bacterial strains”. African Journal of Biotechnology, 10(2), 8359-8372, 2011.
  • Akyol E, Bozkaya O, Dogan NM. “Strengthening sandy soils by microbial methods”. Arabian Journal of Geosciences, 10(327), 1-8, 2017.
  • American Public Health Association (APHA). “Standard methods for the Examination of Water and Wastewater”. Washington, USA, 17th ed.,1989.
  • Weatherburn MW. “Phenol-hypochlorite reaction for determination of ammonia”. Analytical Chemistry, 39(8), 971-974, 1967.
  • Armstrong JM, Myers DV, Verpoorte JA, Edsall JT. “Purification and properties of human erythrocyte carbonic anhydrases”. Journal of Biological Chemistry, 241(21), 5137-5149, 1966.
  • Sun X, Miao L, Tong T, Wang C. “Study of the effect of temperature on microbially induced carbonate precipitation”. Acta Geotechnica, 14(3), 627-638, 2019.
  • Omoregie AI, Ong DEL, Nissom PM. “Assessing ureolytic bacteria with calcifying abilities isolated from limestone caves for biocalcification”. Letters in Applied Microbiology, 68 (2), 173-181, 2018.
  • Mitchell AC, Ferris FG. “The Ca precipitation into calcite precipitates induced by bacterial ureolysis in artificial ground water: temperature and kinetics dependence”. Geochimica Cosmochimica Acta, 69, 4199-4210, 2005.
  • Rodriguez-Navarro C, Jimenez-Lopez C, Rodriguez-Navarro A, Gonzalez-Munöz MT, Rodriguez-Gallego M. “Bacterially mediated mineralization of vaterite”. Geochimica Cosmochimica Acta, 71 (5), 1197-1213, 2007.
  • Stocks-Fischer S, Galinate JK, Bang SS. “Microbiological precipitation of CaCO3”. Soil Biology and Biochemistry, 31 (11), 1563-1571, 1999.
  • Anne S, Rozen Bamu O, Andreazza P, Rouet JL. “Evidence of a bacterial carbonate coating on plaster samples subjected to the calcite bioconcept biomineralization techniques”. Construction and Building Materials, 24 (6), 1036-1042, 2010.
  • Prah J, Maˇcek J, Draˇzic G. “Precipitation of calcium carbonate from a calcium acetate and ammonium carbamate batch system”. Journal of Crystal Growth, 324 (1), 229-234, 2011.
  • Hammes F, Boon N, de Villiers J, Verstraete W, Siciliano SD. “Strain-Specific ureolytic microbial calcium carbonate precipitation”. Applied and Environmental Microbiology, 69(8), 4901-4909, 2003.
  • Li W, Chen WS, Zhou PP, Cao L, Yu LJ. “Influence of initial pH on the precipitation and crystal morphology of calcium carbonate induced by microbial carbonic anhydrase”. Colloids and Surfaces B: Biointerfaces, 102, 281-287, 2013.
  • Chunxiang Q, Jianyun W, Ruixing W, Liang C. “Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii”. Materials Science and Engineering C, 29(4), 1273-1280, 2009.
  • Obst M, Dynes JJ, Lawrence JR, Swerhone GDW, Benzerara K., Karunakaran C, Kaznatcheev K, Tyliszczak T, Hitchcock AP. “Precipitation of amorphous CaCO3 (aragonite-like) by cyanobacteria: A STXM study of the influence of EPS on the nucleation process”. Geochimica et Cosmochimica Acta, 73 (14), 4180-4198, 2009.
  • Kaur DN, Reddy SM, Mukherjee A. “Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites”. Journal of Microbiology and Biotechnology, 23(5), 707-714, 2013.
  • Priya JN, Kannan M. “Effect of carbonic anhydrase and urease on bacterial calcium carbonate precipitation”. Int. Journal of Pharma and Bio Science, 8(3), 609-614, 2017.
  • Sensoy T, Bozbeyoglu NN, Dogan NM, Bozkaya O, Akyol E. “Characterization of Calcium Carbonate Produced by ureolytic bacteria (Sporocarcina pasteurii ATCC 6453 and Bacillus aerius U2) and Effect of Environmental Conditions on Production of Calcium Carbonate”. https://cest.gnest.org/sites/default/files/presentation_file_list/cest2017_00840_poster_paper.pdf. (19.04. 2017).
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makale
Yazarlar

Naime Nur Bozbeyoğlu Bu kişi benim

Tuğba Şensoy Candoğan Bu kişi benim

Şevki Arslan Bu kişi benim

Buket Kabalay Bu kişi benim

Ömer Bozkaya Bu kişi benim

Erdal Akyol Bu kişi benim

Nazime Mercan Doğan Bu kişi benim

Yayımlanma Tarihi 8 Haziran 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 26 Sayı: 3

Kaynak Göster

APA Bozbeyoğlu, N. N., Şensoy Candoğan, T., Arslan, Ş., Kabalay, B., vd. (2020). Calcium carbonate precipitation by urease and carbonic anhydrase positive bacteria. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, 26(3), 513-518.
AMA Bozbeyoğlu NN, Şensoy Candoğan T, Arslan Ş, Kabalay B, Bozkaya Ö, Akyol E, Mercan Doğan N. Calcium carbonate precipitation by urease and carbonic anhydrase positive bacteria. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi. Haziran 2020;26(3):513-518.
Chicago Bozbeyoğlu, Naime Nur, Tuğba Şensoy Candoğan, Şevki Arslan, Buket Kabalay, Ömer Bozkaya, Erdal Akyol, ve Nazime Mercan Doğan. “Calcium Carbonate Precipitation by Urease and Carbonic Anhydrase Positive Bacteria”. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 26, sy. 3 (Haziran 2020): 513-18.
EndNote Bozbeyoğlu NN, Şensoy Candoğan T, Arslan Ş, Kabalay B, Bozkaya Ö, Akyol E, Mercan Doğan N (01 Haziran 2020) Calcium carbonate precipitation by urease and carbonic anhydrase positive bacteria. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 26 3 513–518.
IEEE N. N. Bozbeyoğlu, T. Şensoy Candoğan, Ş. Arslan, B. Kabalay, Ö. Bozkaya, E. Akyol, ve N. Mercan Doğan, “Calcium carbonate precipitation by urease and carbonic anhydrase positive bacteria”, Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, c. 26, sy. 3, ss. 513–518, 2020.
ISNAD Bozbeyoğlu, Naime Nur vd. “Calcium Carbonate Precipitation by Urease and Carbonic Anhydrase Positive Bacteria”. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 26/3 (Haziran 2020), 513-518.
JAMA Bozbeyoğlu NN, Şensoy Candoğan T, Arslan Ş, Kabalay B, Bozkaya Ö, Akyol E, Mercan Doğan N. Calcium carbonate precipitation by urease and carbonic anhydrase positive bacteria. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi. 2020;26:513–518.
MLA Bozbeyoğlu, Naime Nur vd. “Calcium Carbonate Precipitation by Urease and Carbonic Anhydrase Positive Bacteria”. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, c. 26, sy. 3, 2020, ss. 513-8.
Vancouver Bozbeyoğlu NN, Şensoy Candoğan T, Arslan Ş, Kabalay B, Bozkaya Ö, Akyol E, Mercan Doğan N. Calcium carbonate precipitation by urease and carbonic anhydrase positive bacteria. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi. 2020;26(3):513-8.





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