Sürfaktan protein D düzeyleri ile COVID-19 klinik şiddeti arasindaki ilişkinin değerlendirilmesi: bir vaka kontrol çalışması

Öz

Amaç: Serum surfaktan protein D (SP-D) vücutta viral enfeksiyona karşı koruma, bakteriyel ve fungal klirens,
apoptotik hücrelerin temizlenmesi ve inflamasyonun baskılanması gibi roller oynar. Bu çalışma, SP-D düzeyi ile
koronavirüs hastalığı (COVID-19) şiddeti arasındaki ilişkiyi incelemeyi amaçlamaktadır.
Gereç ve yöntem: Çalışmaya 80 hasta (30 hafif hastalığı ve 50 ağır/kritik COVID-19) ve 50 sağlıklı gönüllü dahil
edildi. Serum örneklerinde SP-D seviyeleri ELISA ile analiz edildi.
Bulgular: SP-D düzeyi ortanca değeri hafif hastalıkta 2,47 (1,67-7,79) ng/ml, ağır/kritik hastalık gruplarında
5,65 (3,09-16,55) ng/ml iken, sağlıklı control grubunda 2,89 (10,8-6,24) ng/ml olarak bulundu. Ağır/kritik hastalık
grubu hem hafif hastalık hem de kontrol grubu ile karşılaştırıldığında SP-D düzeylerindeki farklılıklar istatistiksel
olarak anlamlı bulundu (sırasıyla p=0,007 ve 0,001). ROC analizi, hafif COVID-19 hastalığı hastalarına kıyasla
şiddetli/kritik COVID-19 hastalarında serum SP-D seviyeleri için eğri altında kalan alan daha yüksek saptandı
(AUC=0.691, %95 CI=0,56-0.822; p=0,004). Ayrıca, ağır/kritik hastaları saptamak için SP-D düzeyleri 2,44 ng/
ml düzeyinde (p=0,004) %86 duyarlı ve %51,6 özgül saptandı.
Sonuç: SP-D seviyeleri, COVID-19 hastaları için klinik şiddeti ve prognozu tahmin etmede faydalıdır. SP-D,
klinik şiddeti ve prognozu tahmin etmek için değerli bir biyobelirteçtir.

Anahtar Kelimeler

• Sürfaktan protein D
• COVID-19
• pnömoni

Evaluation of the relationship between surfactant protein D levels and COVID-19 clinical severity: a case-control study

Öz

Purpose: Serum surfactant protein D (SP-D) plays roles in the body such as protection against viral infection,
bacterial and fungal clearance, clearance of apoptotic cells and suppression of inflammation. This study aims to
examine the relationship between SP-D level and coronavirus disease (COVID-19) severity.
Methods: 80 patients (30 with mild disease and 50 with severe/critical COVID-19), and 50 healthy volunteers
were enrolled in the study. SP-D levels were analyzed by ELISA in serum samples.
Results: The median of SP-D was found to be 2.47 (1.67-7.79) ng/ml in mild disease and 5.65 (3.09-16.55) ng/
ml in severe/critical disease groups, while 2.89 (10.8-6.24) ng/ml in the healthy controls. The differences in SP-D
levels between the severe/critical disease group compared to both mild disease and control groups were found
statistically significant (p=0.007 and 0.001, respectively). ROC analysis showed greater AUC for the serum
SP-D levels of the severe/critical COVID-19 patients compared to mild COVID-19 disease patients (AUC=0,691,
95% CI=0.56-0,822; p=0.004). Furthermore, SP-D levels were 86% sensitive and 51.6% specific at 2.44 ng/ml
level (p=0.004) to detect severe/critical patients.
Conclusion: SP-D levels is useful for COVID-19 patients in the prediction of clinical severity and prognosis.
SP-D is a valuable biomarker for predicting the clinical severity and prognosis.

Anahtar Kelimeler

• Serum surfactant protein D
• COVID-19
• pneumonia
• pnömoni

Kaynakça

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