Verbascoside, 3T3-L1 hipertrofik hücrelerinde lipolizle ilişkili genlerin ekspresyonunda değişiklik yoluyla lipolizi artırır

Öz

Amaç: Son zamanlarda yapılan çalışmalar, obezite ile mücadele için yağ yakıcı özelliklere sahip doğal, toksik olmayan polifenollerin kullanımında umut verici sonuçlar göstermiştir. Bu çalışmanın amacı, Verbascoside (VB) tedavisine yanıt olarak hipertrofik 3T3-L1 adipositlerin gen ekspresyon profilindeki değişiklikleri ve yağ metabolizmasında önemli rol oynayan 5' AMP ile aktive olan protein kinaz (AMPK), peroksizom proliferatör ile aktive olan reseptör gama (PPAR-γ), hormona duyarlı lipaz (HSL) ve lipid damlacığı ile ilişkili protein (perilipin1, PLIN1) mRNA seviyelerini araştırmaktır. Gereç ve yöntem: Bu amaçla, 3T3-L1 preadipositleri farklılaştırıldı ve hipertrofiye edildi. Hücrelerdeki trigliserit birikimi Oil Red O boyaması kullanılarak değerlendirildi. Hipertrofik 3T3-L1 hücreleri 48 saat boyunca VB ile inkübe edildi. Bulgular: Bu sürenin sonunda yapılan mikroarray analizi, VB'nin lipoliz süreçlerinde yer alan birçok proteinin mRNA seviyelerini artırdığını, ancak PPARγ, AMPK, HSL ve PLIN1 ekspresyonunu değiştirmediğini gösterdi. Bunu doğrulamak için yaptığımız RT-PCR’a göre PPARγ, AMPK, HSL ve PLIN1 ekspresyon seviyelerinin VB tedavisi ile değiştiğini belirledik. Sonuçlarımız, VB uygulamasının lipolitik süreçlerde yer alan genlerin ifadesinde kontrole kıyasla önemli bir artışa neden olduğunu göstermiştir. Sonuç: Bu çalışmanın bulguları, VB'nin lipolitik süreçlerde yer alan önemli hücre içi proteinler aracılığıyla aktif bir rol oynayabileceğini göstermektedir.

Anahtar Kelimeler

• Verbascoside
• polifenol
• adipoz
• lipoliz
• 3T3-L1

Verbascoside promotes lipolysis in 3T3-L1 hypertrophic cells through alteration in the expression of lipolysis-related genes

Öz

Purpose: Recent studies have shown promising outcomes in the utilization of natural, non-toxic polyphenols with fat-burning properties to combat obesity. The aim of this study was to investigate the changes in the gene expression profile of hypertrophic 3T3-L1 adipocytes in response to Verbascoside (VB) treatment and the mRNA levels of 5' AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor gamma (PPAR-γ), hormone-sensitive lipase (HSL), and lipid droplet-associated protein (perilipin1, PLIN1), which play an important role in fat metabolism. Materials and methods: For this purpose, 3T3-L1 preadipocytes were differentiated and hypertrophied. Triglyceride accumulation in the cells was evaluated using Oil Red O staining. The hypertrophic 3T3-L1 cells were incubated with VB for 48 h. Results: Microarray analysis at the end of this period showed that VB increased the mRNA levels of many proteins involved in lipolysis processes, but did not change the expression of PPARγ, AMPK, HSL and PLIN1. Therefore, we confirmed by RT-PCR that PPARγ, AMPK, HSL, and PLIN1 expression levels were altered by VB treatment. Our results showed that VB treatment caused a significant increase in the expression of genes involved in lipolytic processes compared to the control. Conclusion: The findings of this study suggest that VB may play an active role through important intracellular proteins involved in lipolytic processes.

Anahtar Kelimeler

• Verbascoside
• polyphenol
• adipose
• lipolysis
• 3T3-L1

Kaynakça

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