Biochemical and histomorphometric comparison of antioxidant (Taurine) and ozone therapy effects on ESWL-induced oxidative damage in rabbit kidney tissue
Öz
Purpose: This study aimed to investigate the role of free radicals in kidney injury induced by extracorporeal shock wave lithotripsy (ESWL) and to compare the protective effects of taurine and ozone therapy.
Methods: Thirty male New Zealand rabbits were divided into five groups: control, ESWL, ESWL + ozone, ESWL + taurine, and ESWL + ozone + taurine. ESWL was applied in a single session at 14 kV and 2500 shocks. Ozone was administered intraperitoneally and taurine orally for 15 days. Histopathological evaluations and biochemical analyses, including MDA, SOD, CAT, and GSH-Px levels, were performed on renal tissues.
Results: ESWL significantly increased tissue MDA levels and decreased antioxidant enzyme levels. Taurine and ozone treatments reduced oxidative stress by lowering MDA levels and enhancing antioxidant enzyme activity. Histologically, less tissue damage was observed in the treatment groups.
Conclusion: Taurine and ozone therapies effectively reduced ESWL-induced kidney damage both biochemically and histologically. These findings support the potential protective role of these agents in clinical settings.
Anahtar Kelimeler
Lithotripsy extracorporeal, kidney injuries, oxidative stress, taurine, ozone therapy
Kaynakça
- Hill AJ, Basourakos SP, Lewicki P, et al. Incidence of Kidney Stones in the United States: The Continuous National Health and Nutrition Examination Survey. J Urol. 2022;207(4):851-856. doi:10.1097/JU.0000000000002331
- Walsh PC, Retik AB, Vaughan ED, Wein AJ. Urinary stone disease and endourology. In: Campbell’s Urology. 8th ed. Philadelphia, PA: Saunders; 2006.
- Chow GK, Streem SB. Extracorporeal lithotripsy: update on technology. Urol Clin North Am. 2000;27(2):315 322. doi:10.1016 /s0094-0143(05)70260-3
- Kaude JV, Williams CM, Millner MR, Scott KN, Finlayson B. Renal morphology and function immediately after extracorporeal shock wave lithotripsy. AJR Am J Roentgenol. 1985;145(2):305 313. doi:10.2214/ajr.145.2.305
- Rubin JI, Arger PH, Pollack HM, Banner MP, Coleman BG, Mintz MC, VanArsdalen KN. Kidney changes after extracorporeal shock wave lithotripsy: CT evaluation. Radiology. 1987;162(1):21 24.
- Delius M, Jordan M, Eizenhoefer H, et al. Biological effects of shock waves: kidney haemorrhage by shock waves in dogs—administration rate dependence. Ultrasound Med Biol. 1988;14(7):689 694. doi:10.1016/0301-5629(90)90024-7
- Gillenwater JY. Extracorporeal shock wave lithotripsy for the treatment of urinary calculi. In: Gillenwater JY, Grayhack JT, Howards SS, Duckett JW, eds. Adult and Pediatric Urology. 2nd ed. St Louis, MO: Mosby Year Book; 1991:695 710.
- Stoller ML. Extracorporeal shock wave lithotripsy. In: Tanago EA, McAninch JW, eds. Smith’s General Urology. 14th ed. Stamford, CT: Appleton & Lange; 1995:305 313.
- Sharples EJ, Patel N, Brown P, et al. Erythropoietin protects the kidney against the injury and dysfunction caused by ischemia reperfusion. J Am Soc Nephrol. 2004;15(7):2115 2124. doi:10.1097/01.ASN.0000135059.67385.5D
- Yagi K. Lipid peroxides in hepatic, gastrointestinal and pancreatic diseases. Adv Exp Med Biol. 1994;366:165 169.
