Biochemical and histomorphometric comparison of antioxidant (Taurine) and ozone therapy effects on ESWL-induced oxidative damage in rabbit kidney tissue

Öz

Purpose: This study aimed to investigate the role of free radicals in kidney injury induced by extracorporeal shock wave lithotripsy (ESWL) and to compare the protective effects of taurine and ozone therapy.

Methods: Thirty male New Zealand rabbits were divided into five groups: control, ESWL, ESWL + ozone, ESWL + taurine, and ESWL + ozone + taurine. ESWL was applied in a single session at 14 kV and 2500 shocks. Ozone was administered intraperitoneally and taurine orally for 15 days. Histopathological evaluations and biochemical analyses, including MDA, SOD, CAT, and GSH-Px levels, were performed on renal tissues.

Results: ESWL significantly increased tissue MDA levels and decreased antioxidant enzyme levels. Taurine and ozone treatments reduced oxidative stress by lowering MDA levels and enhancing antioxidant enzyme activity. Histologically, less tissue damage was observed in the treatment groups.

Conclusion: Taurine and ozone therapies effectively reduced ESWL-induced kidney damage both biochemically and histologically. These findings support the potential protective role of these agents in clinical settings.

Anahtar Kelimeler

• Lithotripsy extracorporeal
• kidney injuries
• oxidative stress
• taurine
• ozone therapy

Tavşan böbrek dokusunda eswl ile indüklenen oksidatif hasar üzerine antioksidan (Taurin) ve ozon terapi etkilerinin biyokimyasal ve histomorfometrik karşılaştırılması

Öz

Amaç: Bu çalışmada, litotripsi sonrası oluşan böbrek hasarında serbest radikallerin rolü ve bu hasarın azaltılmasında taurin ve ozon tedavisinin etkileri karşılaştırılmıştır.

Gereç ve yöntem: Otuz erkek Yeni Zelanda tavşanı beş gruba ayrıldı: kontrol, ESWL, ESWL + ozon, ESWL + taurin ve ESWL + ozon + taurin. ESWL uygulaması tek seans 14 kV enerjiyle, 2500 atım olarak gerçekleştirildi. Ozon tedavisi intraperitoneal olarak, taurin ise oral yolla 15 gün süreyle verildi. Histopatolojik değerlendirme yanı sıra doku düzeyinde MDA, SOD, CAT, GSH-Px gibi biyokimyasal parametreler ölçüldü.

Bulgular: ESWL sonrası doku MDA düzeyleri anlamlı şekilde artarken, antioksidan enzim düzeyleri azaldı. Taurin ve ozon tedavileri, MDA düzeylerini düşürüp antioksidan enzim düzeylerini artırarak oksidatif stresi azalttı. Histopatolojik olarak da tedavi gruplarında daha az hasar izlendi.

Sonuç: Taurin ve ozon tedavisi, ESWL'nin neden olduğu böbrek hasarını hem biyokimyasal hem de histopatolojik olarak azaltmada etkili bulunmuştur. Bu sonuçlar, klinik uygulamalarda bu ajanların koruyucu potansiyelini desteklemektedir.

Anahtar Kelimeler

• Litotripsi ekstrakorporeal
• böbrek yaralanmaları
• oksidatif stres
• taurin
• ozon terapisi

Kaynakça

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