Metotreksat kaynaklı beyin hasarına karşı bromelainin potansiyel faydalı etkilerinin araştırılması

Öz

Amaç: Metotreksat, bazı kanser türleri ve romatoid artrit başta olmak üzere çeşitli hastalıkların tedavisinde kullanılan bir folik asit antagonistidir. Ancak, metotreksat kullanımıyla birlikte görülen ve nörotoksisiteyi de içeren birçok yan etki önemli bir klinik problemdir. Ananas bitkisinden elde edilen bromelain, radikal süpürücü ve lipit peroksidasyonu önleyici etkileri olan enzimatik bir karışımdır.

Gereç ve Yöntem: 28 adet rat rastgele 4 gruba bölündü (n=7). Birinci grup kontrol grubu olarak tutuldu. İkinci grup bromelain grubu olarak belirlendi ve bu gruptaki ratlara 14 gün boyunca 200 mg/kg/gün bromelain verildi. Üçüncü grup metotreksat grubu olarak belirlendi ve bu gruptaki ratlara üçüncü gün tek doz 20 mg/kg metotreksat verildi. Dördüncü grup metotreksat+bromelain grubu olarak belirlendi ve gruptaki ratlara metotreksat ve bromelain eşdeğer dozlarda birlikte verildi.

Bulgular: Metotreksat uygulaması gruplar arasında tiyobarbitürik asit reaktif substans seviyeleri açısından herhangi bir değişikliğe neden olmadı ancak redükte glutatyon seviyesinde ve süperoksit dismutaz, glutatyon peroksidaz ve katalaz aktivitelerinde kontrol grubu ile karşılaştırıldığında istatistiksel olarak anlamlı bir azalmaya neden oldu. Metotreksat ile birlikte bromelain uygulanması redükte glutatyon seviyesini ve süperoksit dismutaz aktivitesini metotreksat grubu ile karşılaştırıldığında anlamlı şekilde arttırdı ancak glutatyon peroksidaz ve katalaz aktivitelerindeki artış istatistiki olarak anlamlı değildi. Beyin dokusu histolojik açıdan da değerlendirildi. metotreksat uygulamasının beyinde histopatolojik olarak belirgin bir lezyon oluşumuna yol açmadığı görüldü. Sonuç: Bulgularımız bromelain kullanımının nörolojik hasara karşı metotreksat ile tedavi edilen hastalarda fayda sağlayabileceğini desteklemektedir.

Anahtar Kelimeler

• metotreksat,
• bromelain
• beyin hasarı
• oksidatif stres

Investigation of potential beneficial effects of bromelain against methotrexate-induced brain injury

Öz

Abstract Purpose: Methotrexate is a folic acid antagonist used in the treatment of various diseases, especially some types of cancer and rheumatoid arthritis. However, many side effects associated with methotrexate use, including neurotoxicity, are an important clinical problem. Bromelain obtained from the pineapple plant is an enzymatic mixture with radical scavenging and anti-lipid peroxidation effects.

Materials and methods: 28 rats were randomly divided into 4 groups (n=7). The first group was kept as the control group. The second group was determined as the bromelain group and 200 mg/kg/day bromelain was given to the rats in this group for 14 days. The third group was determined as methotrexate group and a single dose of 20 mg/kg methotrexate was given to the rats in this group on the third day. The fourth group was determined as methotrexate + bromelain group, and the rats in the group were given methotrexate and bromelain in equivalent doses together.

Results: Methotrexate administration did not cause any change in thiobarbituric acid reactive substance levels among groups, but caused a statistically significant decrease in reduced glutathione level and superoxide dismutase, glutathione peroxidase and catalase activities compared to the control group. The administration of bromelain with methotrexate significantly increased the reduced glutathione level and superoxide dismutase activity compared to the methotrexate group, but the increase in glutathione peroxidase and catalase activities was not statistically significant. Brain tissue was also evaluated histologically. It was observed that methotrexate administration did not cause a significant lesion formation in the brain histopathologically.

Conclusion: Our findings support that the use of bromelain may benefit patients treated with methotrexate against neurological damage. Keywords: Methotrexate, bromelain, brain injury, oxidative stress.

Anahtar Kelimeler

• Methotrexate
• bromelain
• brain injury
• oxidative stress

Kaynakça

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