Aktif erkek bireylerde tek seans uygulanan izokinetik egzersizin DNA hasarı üzerine etkilerinin incelenmesi

Yıl 2019, Cilt: 12 Sayı: 1, 33 - 40, 18.01.2019

Emine Kılıç Toprak Fatma Ünver Özgen Kılıç Erkek Halil Korkmaz Burak Oymak Yasin Özdemir Alten Oskay Vural Küçükatay Melek Bor Küçükatay

https://doi.org/10.31362/patd.463518

Öz

ÖZET

Amaç: İzokinetik
egzersiz, kas kuvvet dengesizliğini azaltmak ve performansı arttırmak için
kullanılır. Önceki çalışmalarda, farklı egzersiz türlerinin DNA üzerinde
yaptığı değişiklikler araştırılmış olmakla birlikte, izokinetik egzersizin DNA
üzerine etkisi bilinmemektedir. Bu çalışmada, tek seans uygulanan izokinetik
egzersiz protokolünü takiben olası DNA hasarının zamana bağlı değişikliklerinin
incelenmesi amaçlanmıştır.

Gereç ve
Yöntemler: Çalışmamıza 11 sağlıklı aktif erkek
birey dahil edilmiştir (ort yaş 19,45±0,31, vücut kitle indeksi 22,05±0,51 kg/m²). Bireylerin dominant diz fleksiyon/ekstansiyon kuvvetleri izokinetik dinamometre
(Humac Norm Testing Rehabilitation system, CSMI Medikal Solutions, USA)
kullanılarak ölçülmüştür. Quadriceps ve hamstring izokinetik kas kuvvetleri 30^o/s
ve 120^o/s açısal hızlarda eksantrik modda test edilmiştir. Bireylerden egzersiz öncesi, hemen sonrası ve 2 gün
sonra alınan venöz kan
örneklerinde, DNA hasarı belirlenmesinde Comet
analizi kullanılmıştır. İstatistiksel
analiz için Friedman testi kullanılmış, p≤0,05
değerleri istatistiksel olarak anlamlı kabul edilmiştir.

Bulgular ve
Sonuçlar: İzokinetik egzersizin DNA hasarına
etkileri incelendiğinde; kuyruk
uzunluğu (18,76±3,08’e karşın 16,21±1,14; p=0,027)
egzersizden hemen sonra alınan kan
örneklerinde azalırken, 2 gün sonra post-egzersiz döneme göre artmış olarak (16,21±1,14’e
karşın 18,33±1,23; p=0,011)
belirlenmiştir.

Sonuç: Egzersizden hemen sonra azalan kuyruk uzunluğu, uygulanan izokinetik egzersizin genotoksisite üzerine olumlu etkide
bulunabileceğini düşündürmektedir. Olumlu etki 2 gün içinde geri dönmektedir.

Anahtar Kelimeler

İzokinetik egzersiz, DNA hasarı

Investigation of the effects of an acute bout of isokinetic exercise on DNA damage in active male subjects

Öz

Purpose: Isokinetic exercise is used to reduce
strength imbalance and to enhance performance. Although previous studies
presented alterations on DNA induced by different exercise types; effects of
isokinetic exercise on DNA remain unknown. Present study was designed to
explore the time course of possible DNA damage following an acute bout of
isokinetic exercise.

Materials and Methods: 11 healthy,
male, active subjects (mean age 19.45±0.31,
Body mass index 22.05±0.51) were enrolled. They
performed eccentric contractions of knee flexors and extensors with dominant
leg on a dynamometer (Humac Norm Testing Rehabilitation system, CSMI Medikal
Solutions, USA). Isokinetic hamstring, quadriceps strength were recorded at
eccentric (30, 120°s^-1) angular velocities. Blood was obtained before, after and 2 days following the
exercise. DNA damage was
measured by Comet analysis. Friedman test was used for statistical analysis, p≤0.05
was considered statistically significant.

Results: In terms of DNA
damage parameters following isokinetic exercise tail length (18.76±3.08 vs. 16.21±1.14; p=0.027)
was significantly decreased, whereas it was increased again on the 2nd day
following the exercise compared to the post-exercise value (16.21±1.14
vs.18.33±1.23; p=0.011).

Conclusion: Decreased
tail length immediately after exercise suggests that the applied isokinetic
exercise may positively influence genotoxicity. This favorable effect returns
within 2 days. 

Anahtar Kelimeler

Isokinetic exercise test, DNA damage, eccentric exercise

Kaynakça

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