Aktif erkek bireylerde tek seans uygulanan izokinetik egzersizin DNA hasarı üzerine etkilerinin incelenmesi

Öz

ÖZET

Amaç: İzokinetik egzersiz, kas kuvvet dengesizliğini azaltmak ve performansı arttırmak için kullanılır. Önceki çalışmalarda, farklı egzersiz türlerinin DNA üzerinde yaptığı değişiklikler araştırılmış olmakla birlikte, izokinetik egzersizin DNA üzerine etkisi bilinmemektedir. Bu çalışmada, tek seans uygulanan izokinetik egzersiz protokolünü takiben olası DNA hasarının zamana bağlı değişikliklerinin incelenmesi amaçlanmıştır.

Gereç ve Yöntemler: Çalışmamıza 11 sağlıklı aktif erkek birey dahil edilmiştir (ort yaş 19,45±0,31, vücut kitle indeksi 22,05±0,51 kg/m²). Bireylerin dominant diz fleksiyon/ekstansiyon kuvvetleri izokinetik dinamometre (Humac Norm Testing Rehabilitation system, CSMI Medikal Solutions, USA) kullanılarak ölçülmüştür. Quadriceps ve hamstring izokinetik kas kuvvetleri 30^o/s ve 120^o/s açısal hızlarda eksantrik modda test edilmiştir. Bireylerden egzersiz öncesi, hemen sonrası ve 2 gün sonra alınan venöz kan örneklerinde, DNA hasarı belirlenmesinde Comet analizi kullanılmıştır. İstatistiksel analiz için Friedman testi kullanılmış, p≤0,05 değerleri istatistiksel olarak anlamlı kabul edilmiştir.

Bulgular ve Sonuçlar: İzokinetik egzersizin DNA hasarına etkileri incelendiğinde; kuyruk uzunluğu (18,76±3,08’e karşın 16,21±1,14; p=0,027) egzersizden hemen sonra alınan kan örneklerinde azalırken, 2 gün sonra post-egzersiz döneme göre artmış olarak (16,21±1,14’e karşın 18,33±1,23; p=0,011) belirlenmiştir.

Sonuç: Egzersizden hemen sonra azalan kuyruk uzunluğu, uygulanan izokinetik egzersizin genotoksisite üzerine olumlu etkide bulunabileceğini düşündürmektedir. Olumlu etki 2 gün içinde geri dönmektedir.

Anahtar Kelimeler

• İzokinetik egzersiz,DNA hasarı

Investigation of the effects of an acute bout of isokinetic exercise on DNA damage in active male subjects

Abstract

Purpose: Isokinetic exercise is used to reduce strength imbalance and to enhance performance. Although previous studies presented alterations on DNA induced by different exercise types; effects of isokinetic exercise on DNA remain unknown. Present study was designed to explore the time course of possible DNA damage following an acute bout of isokinetic exercise.

Materials and Methods: 11 healthy, male, active subjects (mean age 19.45±0.31, Body mass index 22.05±0.51) were enrolled. They performed eccentric contractions of knee flexors and extensors with dominant leg on a dynamometer (Humac Norm Testing Rehabilitation system, CSMI Medikal Solutions, USA). Isokinetic hamstring, quadriceps strength were recorded at eccentric (30, 120°s^-1) angular velocities. Blood was obtained before, after and 2 days following the exercise. DNA damage was measured by Comet analysis. Friedman test was used for statistical analysis, p≤0.05 was considered statistically significant.

Results: In terms of DNA damage parameters following isokinetic exercise tail length (18.76±3.08 vs. 16.21±1.14; p=0.027) was significantly decreased, whereas it was increased again on the 2nd day following the exercise compared to the post-exercise value (16.21±1.14 vs.18.33±1.23; p=0.011).

Conclusion: Decreased tail length immediately after exercise suggests that the applied isokinetic exercise may positively influence genotoxicity. This favorable effect returns within 2 days. 

Keywords

• Isokinetic exercise test,DNA damage,eccentric exercise

Kaynakça

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