Yıl 2020, Cilt 13 , Sayı 1, Sayfalar 9 - 18 2020-01-21

DEBIO 1143’ün tek başına veya tamoksifenle kombinasyonunun östrojen reseptörü pozitif meme kanseri hücre hatlarındaki etkisi.
The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines.

Berrin TUĞRUL [1] , Merve İŞSEVEN [2]


Amaç: Tamoxifen (TAM) ve DEBIO 1143 (AT-406)’ün artan konsantrasyonlarının tek başına veya birlikte uygulanmasının, MCF-7 ve BT-474 östrojen reseptörü pozitif (ER+) meme kanseri hücre hatlarındaki etkisini araştırmak.

Gereç ve Yöntem: XTT testi, Tamoxifen ve DEBIO 1143’ün tek başına veya birlikte kullanımın 72 saatlik MCF-7 ve BT-474 hücre hatlarındaki hücre canlılığı üzerine olan etkisini belirlemek için kullanıldı. TAM ve DEBIO 1143'ün tek başına / kombinasyonunun her iki hücre hattı üzerindeki etkisini göstermek için çok parametreli apoptoz analiz kiti kullanıldı ve floresan mikroskobik görüntüleme yapıldı.

Bulgular: TAM'ın IC50 değeri MCF-7 ve BT-474 hücre hatlarında sırasıyla 3,8±0,6 mikromolar (µM) ve 18,9±6,7 µM idi. DEBIO 1143'ün IC50 değeri, MCF-7 hücre hattında 15±0,5 uM idi. İlaç kombinasyonuna bağlı sonuçlar her iki hücre hattı için istatistiksel olarak anlamlıydı (p<0,001). Hücre canlılığının azalması apoptozis ile ilişkili bulunmadı.

Sonuç: ER+ meme kanseri hücre hatlarında, kombinasyon halinde uygulanan TAM ve DEBIO 1143 dozlarının, tek başına uygulanmalarına göre hücre canlılığını daha fazla azalttığı belirlenmiştir. Her iki hücre hattında kombinasyon dozlarının uygulanması sinerjik bir etki göstermiştir. Kombine uygulamanın sebep olduğu hücre canlılığındaki azalmanın, apoptoz dışındaki hangi hücre ölüm tipi ile olduğunu belirlemek için ileri araştırmalara ihtiyaç vardır.

Purpose: To investigate the effect of increased concentrations of Tamoxifen (TAM) and DEBIO 1143 (AT-406) administered alone or in combination on cells in MCF-7 and BT-474 estrogen receptor positive (ER +) breast cancer cell lines.

Materials and Methods: The effect of Tamoxifen and DEBIO 1143 administered alone or in combination on cell viability in MCF-7 and BT-474 cell lines at the 72nd hour was assessed by the XTT test. Multi-parameter apoptosis assay kit was used to display the effect of the alone/combination of TAM and DEBIO 1143 on both cell lines. Fluorescence microscobic analysis was performed.

Results: The IC50 value of TAM was 3.8±0.6 micromolar (µM) and 18.9±6.7 µM in the MCF-7 and BT-474 cell lines, respectively. The IC50 value of DEBIO 1143 was 15±0.5 µM in the MCF-7 cell line. The results related to drug combination were statistically significant for both cell lines (p<0.001). The decrease in cell viability was not associated with apoptosis.

Conclusion: In ER + breast cancer cell lines, the combined doses of the TAM and DEBIO 1143 reduced cell viability more than their administration alone. Combined administrations in both cell lines were concluded in a synergistic effect. Further research is needed to determine which cell death type other than apoptosis is associated with a reduction in cell viability caused by combined administration.

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Birincil Dil en
Konular Biyokimya ve Moleküler Biyoloji
Yayımlanma Tarihi Ocak 2020
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0003-0844-7766
Yazar: Berrin TUĞRUL (Sorumlu Yazar)
Kurum: Manisa Celal Bayar University, Faculty of Science and Letters, Department of Biology, Molecular Biology Division, Manisa
Ülke: Turkey


Orcid: 0000-0001-8302-7943
Yazar: Merve İŞSEVEN
Kurum: Manisa Celal Bayar University, Faculty of Science and Letters, Department of Biology, Molecular Biology Division, Manisa
Ülke: Turkey


Destekleyen Kurum Manisa Celal Bayar University Scientific Research Foundation.
Proje Numarası FBE 2016-133.
Tarihler

Yayımlanma Tarihi : 21 Ocak 2020

Bibtex @araştırma makalesi { patd623005, journal = {Pamukkale Tıp Dergisi}, issn = {}, eissn = {1308-0865}, address = {Pamukkale Üniversitesi Tıp Fakültesi Eğitim Blokları Kınıklı kampüsü 20070 Kınıklı, Denizli}, publisher = {Pamukkale Üniversitesi}, year = {2020}, volume = {13}, pages = {9 - 18}, doi = {10.31362/patd.623005}, title = {The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines.}, key = {cite}, author = {TUĞRUL, Berrin and İŞSEVEN, Merve} }
APA TUĞRUL, B , İŞSEVEN, M . (2020). The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines.. Pamukkale Tıp Dergisi , 13 (1) , 9-18 . DOI: 10.31362/patd.623005
MLA TUĞRUL, B , İŞSEVEN, M . "The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines.". Pamukkale Tıp Dergisi 13 (2020 ): 9-18 <https://dergipark.org.tr/tr/pub/patd/issue/51998/623005>
Chicago TUĞRUL, B , İŞSEVEN, M . "The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines.". Pamukkale Tıp Dergisi 13 (2020 ): 9-18
RIS TY - JOUR T1 - The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines. AU - Berrin TUĞRUL , Merve İŞSEVEN Y1 - 2020 PY - 2020 N1 - doi: 10.31362/patd.623005 DO - 10.31362/patd.623005 T2 - Pamukkale Tıp Dergisi JF - Journal JO - JOR SP - 9 EP - 18 VL - 13 IS - 1 SN - -1308-0865 M3 - doi: 10.31362/patd.623005 UR - https://doi.org/10.31362/patd.623005 Y2 - 2019 ER -
EndNote %0 Pamukkale Tıp Dergisi The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines. %A Berrin TUĞRUL , Merve İŞSEVEN %T The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines. %D 2020 %J Pamukkale Tıp Dergisi %P -1308-0865 %V 13 %N 1 %R doi: 10.31362/patd.623005 %U 10.31362/patd.623005
ISNAD TUĞRUL, Berrin , İŞSEVEN, Merve . "The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines.". Pamukkale Tıp Dergisi 13 / 1 (Ocak 2020): 9-18 . https://doi.org/10.31362/patd.623005
AMA TUĞRUL B , İŞSEVEN M . The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines.. Pamukkale Tıp Dergisi. 2020; 13(1): 9-18.
Vancouver TUĞRUL B , İŞSEVEN M . The effect of DEBIO 1143 usage alone or in combination with Tamoxifen on estrogen receptor positive breast cancer cell lines.. Pamukkale Tıp Dergisi. 2020; 13(1): 18-9.