Dondurulmuş ve çözülmüş ovaryum dokusunda mTOR ve p-mTOR ekspresyonunun immünohistokimyasal olarak gösterilmesi

Yıl 2026, Cilt: 19 Sayı: 1, 3 - 3

Umray Dede , Murat Serkant Ünal , Nazlı Çil , Gülçin Abban Mete

Öz

Amaç: Ovarian kriyoprezervasyon, doğurganlığı korumak için tercih edilen toksik etkileri en aza indiren oldukça faydalı bir yöntemdir. Mammalian target of rapamycin (mTOR) yolağı hücre büyümesi, çoğalması, otofaji, besin sinyalizasyonu ve hayatta kalması için kritik öneme sahiptir. Amacımız immünohistokimyasal yöntemle overin dondurulmasından önce ve sonra mTOR ve Fosforile (p-mTOR) ekspresyonlarını araştırmaktır.
Gereç ve yöntem: Kontrol (C; n=6) grubu ve vitrifikasyon ve çözdürme grubu (V/T; n=6) over grupları. C grubu tamponlu formalin içinde 48 saat fikse edildi. V/T grubu -196ºC'de sıvı nitrojen içinde donduruldu. Bir hafta sonra dokular çözüldü. Her iki grupta da parafin takibi yapıldı. Ovaryumların Hematoksilen ve Eozin ile boyanmasını takiben folikül sayımı yapıldı. mTOR ve p-mTOR ekspresyonu için immünohistokimya incelemesi yapıldı.
Bulgular: Folikül sayıları gruplar arasında istatistiksel olarak farklı değildi (p>0.05). C ve V/T grubu overlerdeki foliküllerde güçlü mTOR ekspresyonu gözlendi. Kontrol grubunda oositlerde ve granüloza hücrelerinde pozitif p-mTOR ekspresyonu gözlenirken, V/T grubunda ekspresyon negatifti.
Sonuç: Kriyoprezervasyon yöntemleri over dokusunda mTOR ekspresyonunu değiştirmemiştir. Bununla birlikte, dondurulmuş ovaryum dokularında oosit kalitesi ile ilişkili p-mTOR aktivitesinin olmaması, kriyoprezervasyonun oosit kalitesini etkilediğini düşündürmüştür.

Anahtar Kelimeler

Ovaryum , Kriyoprezervasyon , Vitrifikasyon , mTOR , p-mTOR

Etik Beyan

Pamukkale Üniversitesi Deney Hayvanları Etik Kurulu (Onay numarası: 2016/24).

Destekleyen Kurum

Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi (2017SABE007)

Proje Numarası

Pamukkale University Scientific Research Projects Coordination Unit (grant number 2017SABE007)

Immunohistochemical demonstration of mTOR and p-mTOR expression in frozen and thawed ovarian tissue

Öz

Purpose: The Ovarian cryopreservation is highly useful method is minimizing toxic due to prefered for protect fertility. Mammalian target of rapamycin (mTOR) pathway is critical to cell growth, proliferation, autophagy, nutrient signaling, and survival. Our aim was to investigate the mTOR and Phosphorylated (p-mTOR) expressions before and after frozen of the ovary by immunohistochemical method.
Material and methods: Control (C; n=6) group and vitrification and thawing group (V/T; n=6) groups of ovaries. C group fixed for 48 hours in buffered formalin. V/T group were frozen in liquid nitrogen at -196ºC. One week later the tissues were thawed. Both groups performed paraffin tracking. Following the Hematoxylin and Eosin staining of the ovaries, a follicle count was made. Immunohistochemistry examination for mTOR and p-mTOR expression were performed.
Results: Follicle numbers were not statistically different in the groups (p>0.05). The strong expression of mTOR was observed in follicles in C and V/T group ovary. Positive p-mTOR expression was observed in oocytes and granulosa cells in the control group, whereas the expression was negative in the V/T group.
Conclusion: Cryopreservation methods did not induce to alter mTOR expression in ovarian tissue. However, the absence of p-mTOR activity associated with oocyte quality in frozen ovarian tissues suggested that cryopreservation effected the quality of oocyte.

Anahtar Kelimeler

Ovary , Cryopreservation , Vitrification , mTOR , p-mTOR

Etik Beyan

Pamukkale University Experiments Ethics Committee (Approval number: 2016/24).

Destekleyen Kurum

Pamukkale University Scientific Research Projects Coordination Unit (grant number 2017SABE007)

Proje Numarası

Pamukkale University Scientific Research Projects Coordination Unit (grant number 2017SABE007)

Kaynakça

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