Using Inorganic Nanoparticle-Based Drug Delivery Systems against Human Colon Cancer Cells: Effect of Particle Size on Anticancer Activity

Yıl 2020, , 171 - 179, 01.03.2020

Cenk Dağlıoğlu

https://doi.org/10.2339/politeknik.496351

Cited By: 1

Öz

Today’s nanoparticle
technology enables the synthesis of nanoparticle-based drug delivery systems with
desired size, shape, and materials especially for the applications of cancer nanomedicine.
Thereby, understanding impact of particle sizes on anticancer activity will
contribute to development of new drug delivery systems in cancer therapy. For this reason, in this
study, two different sized nanoparticles (with ~55 and 314 nm) were used as
drug delivery systems and the effects of their size on the cellular uptake, cytotoxicity
and apoptosis were investigated against the human colon carcinoma Caco-2 and HCT-116
cells. The results demonstrated that small nanoparticles promoted fast
nanoparticle accumulation in both
cancer cells in comparison to large particles. Small nanoparticles
exhibited higher cytotoxicity in cancer cells with lower half maximal
inhibitory concentration (IC₅₀) values than large nanoparticles in
48 h. On the other hand, both nanoparticles showed similar IC₅₀
values after 72 h prolonged exposure. Moreover, it was found that small
nanoparticles increased the number of apoptotic cells in 24 h, whereas large nanoparticles
induced apoptosis when exposure time increased to 72 h. These observations show
that small sized drug delivery systems could be more efficient for improving
the anticancer effects of chemotherapeutic drugs against human colon carcinoma
as compared to large sized drug delivery systems.

Anahtar Kelimeler

Drug delivery systems, particle size, colon cancer, Caco-2 cells, HCT-116 cells

İnsan Kolon Kanseri Hücrelerine Karşı İnorganik Nanopartikül-Temelli İlaç Taşıyıcı Sistemlerin Kullanılması: Partikül Büyüklüğünün Antikanser Aktivitesine Etkisi

Öz

Günümüz nanopartikül teknolojisi, özellikle kanser
nanotıp uygulamalarında kullanılmak üzere istenilen boyut, şekil ve malzemeye
sahip nanopartikül-temelli ilaç taşıyıcı sistemlerinin sentezine olanak
sağlamaktadır. Dolayısıyla, partikül boyutlarının antikanser aktivite
üzerindeki etkisini anlamak, kanser tedavisinde yeni ilaç taşıyıcı sistemlerin
geliştirilmesine katkıda bulunacaktır. Bu nedenle, bu çalışmada, ilaç taşıyıcı
sistemler olarak iki farklı büyüklükteki inorganik temelli nanopartiküller (~
55 ve 314 nm) kullanıldı ve boyutlarının hücresel birikim, sitotoksisite ve
apoptoz üzerindeki etkileri insan kolon kanseri Caco-2 ve HCT-116 hücrelerine karşı
araştırıldı. Elde edilen sonuçlar, büyük nanopartiküllerle karşılaştırıldığında
küçük nanopartiküllerin her iki kanser hücresinde de hızlı nanopartikül birikimini
desteklediğini gösterdi. Küçük nanopartiküller, büyük nanopartiküllere göre 48
saat içinde daha düşük yarı-maksimum
inhibisyon konsantrasyonu (IC₅₀) değerleri ile kanser hücrelerinde
daha yüksek sitotoksisite sergiledi.
Öte yandan,
her iki nanopartikül de 72 saate varan inkübasyon süreleri sonrası benzer IC₅₀
değerleri gösterdi. Ayrıca, küçük nanopartiküller 24 saatte apoptotik hücrelerin sayısını artırırken,
büyük nanopartiküllerin 72 saatlik süre içerisinde apoptozu indüklediği belirlendi. Bu gözlemler, küçük boyutlu ilaç taşıma sistemlerinin,
büyük boyutlu ilaç taşıma sistemleri ile karşılaştırıldığında, insan kolon
kanseri hücrelerinde kemoterapötik ilaçların antikanser etkilerini artırmada
daha verimli olduğunu göstermektedir.

Anahtar Kelimeler

İlaç taşıma sistemleri, partikül boyutu, kolon kanseri, Caco-2 hücreleri, HCT-116 hücreleri

Kaynakça

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