Pediastrum boryanum (Turpin) Meneghini’nin Antibakteriyal ve Antifungal Etkilerinin Araştırılması

Öz

Bu çalışmada, Yeşilırmak Nehri bentik ve pelajik habitatlarından alınarak kültürü yapılan Pediastrum boryanum (Turpin) Meneghini (Synonim Pseudopediastrum boryanum (Turpin) E.Hegewald)’un tris-HCL, n-butanol ve etanol ekstraktlarının çeşitli mikroorganizma türleri üzerinde antibakteriyal ve antifungal etkileri araştırılmıştır. Antimikrobiyal aktivite testleri, disk difüzyon yöntemi ile Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Listeria monocytogenes ATCC 7644, Escherichia coli O 157:H7, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium CCM 5445, Candida albicans ATCC 10239 mikroorganizmaları üzerinde denendi. Sonuç olarak, antibakteriyal aktivite testlerinde P. boryanum’un tampon ekstraktının, Listeria monocytogenes ATCC 7644 üzerinde oldukça etkili antibakteriyal özelliğe sahip olduğu tespit edildi. Escherichia coli O 157:H7 suşunun en duyarlı mikroorganizma, Bacillus subtilis ATCC 6633 suşunun ise en dirençli mikroorganizma olduğu; en etkili çözücünün 0,5 M Tris-HCL pH:8,00 olduğu kaydedildi. Antifungal aktivite testleri ile P. boryanum’un etanol ekstraktının, Candida albicans ATCC 10239 üzerinde oldukça etkili antifungal özelliğe sahip olduğu görüldü.

Anahtar Kelimeler

• Pediastrum boryanum
• Pseudopediastrum boryanum
• Antibakteriyal Aktivite
• Antifungal Aktivite
• Disk Difüzyon Metodu

Investigation of Antibacterial and Antifungal Effects of Pediastrum boryanum (Turpin) Meneghini

Öz

In this study, the antibacterial and antifungal effects of tris-HCL, n-butanol and ethanol extracts of Pediastrum boryanum (Turpin) Meneghini (Synonym Pseudopediastrum boryanum (Turpin) E. Hegewald), cultured from benthic and pelagic habitats of the Yeşilırmak River, on various microorganism species were investigated. Antimicrobial activity tests were performed on the microorganisms Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Listeria monocytogenes ATCC 7644, Escherichia coli O 157:H7, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium CCM 5445, Candida albicans ATCC 10239 by disk diffusion method. As a result, in antibacterial activity tests, it was determined that the buffer extract of P. boryanum had highly effective antibacterial properties on Listeria monocytogenes ATCC 7644. It was recorded that Escherichia coli O 157:H7 strain was the most susceptible microorganism, while Bacillus subtilis ATCC 6633 strain was the most resistant microorganism; the most effective solvent was 0.5 M Tris-HCL pH: 8.00. In antifungal activity tests, it was seen that the ethanol extract of P. boryanum had highly effective antifungal properties on Candida albicans ATCC 10239.

Anahtar Kelimeler

• Pediastrum boryanum
• Pseudopediastrum boryanum
• Antibacterial Activity
• Antifungal Activity
• Disk Diffusion Method

Kaynakça

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