Protective Effects of Aronia melanocarpa Extract against Cyclophosphamide-Induced Delayed Toxicity on the Bladder

Yıl 2025, Cilt: 15 Sayı: 1, 36 - 42, 03.01.2025

Hümeyra Çelik , Şeyda Karabörk , Aslıhan Şaylan , Ayhan Çetinkaya

https://doi.org/10.33631/sabd.1404408

Öz

Aim: Aronia melanocarpa is a red-purple medicinal fruit known for its therapeutic properties in the urinary system by anti-inflammatory effects with high antioxidant content. The aim of the study is to show the supportive effect of Aronia melanocarpa extract delayed toxicity on the bladder induced by cyclophosphamide (CYC) that an antineoplastic agent.
Material and Methods: In the study three groups were constituted control (n=7), CYC(urotoxicity group, n=7) and CYC+ARONIA(treatment group, n=7). 100 mg/kg CYC intraperitoneally were given to CYC and CYC+ARONIA groups and waited for 4 weeks to be created delayed toxicity. At the end of the 4 weeks, 200 mg/kg Aronia melanocarpa was administered 15 times by oral gavage every other different day to CYC+ARONIA group (1 month in total). Sacrification was performed and after serum and urine samples were taken, the bladder was released from the sphincter region with curved-tipped forceps. Bladder tissues were investigated histologically. P38 mitogen activated preotein kinase (P38 MAPK), total antioxidant (TAS) and oxidant (TOS) status were evaluated in serum and urine samples.
Results: In histology, histological damage in the bladder continued in the CYC group, while Aronia melanocarpa treatment caused healing in the bladder tissue in the CYC+ARONIA group. No difference was found between the groups in terms of P38 MAPK, TAS and TOS in serum and urine samples.
Conclusion: According to the experimental results, the fact that Aronia melanocarpa extract improves the histological damage caused by CYC in the delayed period, and the serum and urine findings were the same as the controls, brought up the therapeutic effect of Aronia melanocarpa in urotoxicity.

Anahtar Kelimeler

cyclophosphamide, Aronia melanocarpa, P38 MAPK, TAS-TOS

Siklofosfamidin Gecikmiş Toksisitesine Karşı Aronia melanocarpa Ekstraktının Mesane Üzerine Koruyucu Etkileri

Öz

Amaç: Aronia melanocarpa yüksek antioksidan içeriğiyle antiinflamatuar etkiler göstererek üriner sistemde tedavi edici özellikleri bilinen kırmızı-mor renkli tıbbi bir meyvedir. Çalışmanın amacı antineoplastik bir ajan olan siklofosfamidin (CYC) mesanede oluşturduğu gecikmiş toksisitede Aronia melanocarpa ekstraktının destekleyici etkisini göstermektir.
Gereç ve Yöntemler: Çalışmada kontrol (n=7), CYC (ürotoksisite grubu, n=7) ve CYC+ARONIA (tedavi grubu, n=7) olacak şekilde üç grup oluşturuldu. CYC ve CYC+ARONIA gruplarına 100 mg/kg CYC intraperitoneal olarak 1 doz verildikten sonra gecikmiş toksisite oluşması için 4 hafta beklenildi. 4 haftanın sonunda Aronia melanocarpa ekstratı oral gavaj ile gün aşırı toplamda 1 ay olacak şekilde 15 kez 200 mg/kg dozunda CYC+ARONIA grubuna verildi. Sakrifikasyonda serum ve idrar örnekleri alındıktan sonra mesane dokusu sfinkter bölgesinden eğimli uçlu forsepsle serbestleştirilerek alındı. Mesane dokusu histolojik olarak değerlendirildi. Enflamatuvar belirteç olan P38 MAP Kinaz, toplam antioksidan (TAS) ve oksidan (TOS) durumu serum ve idrarda değerlendirildi.
Bulgular: Histolojide CYC grubunda mesanedeki histolojik hasar devam ederken, CYC+ARONIA grubunda Aronia melanocarpa tedavisi mesane dokusunda iyileşmeye sebep oldu. Serum ve idrar örneklerinde P38 MAPK, TAS ve TOS açısından gruplar arasında fark bulunmadı.
Sonuç: Deneysel sonuçlara göre Aronia melanocarpa ekstraktının CYC'nin neden olduğu histolojik hasarı geç dönemde iyileştirmesi, serum ve idrar bulgularının kontrollerle aynı olması Aronia melanocarpa'nın ürotoksisitede terapötik etkisini gündeme getirmiştir.

Anahtar Kelimeler

Aronia melanocarpa, P38 MAPK, TAS-TOS, siklofosfamid

Kaynakça

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