Polymerase Chain Reaction Inhibitors

Yıl 2018, Cilt: 1 Sayı: 1, 31 - 36, 01.10.2018

Begüm Maşlak A Funda Bağcıgil

Öz

Polymerase chain reaction (PCR) is a method which is based on the principle that the desired gene regions are copied and reproduced in-vitro conditions and that the obtained products are made visible. PCR has advantages such as rapidity and specificity related with selected primers and high sensitivity. However, it has some disadvantages. The most important disadvantages are; difficulties encountered in evaluating the results, false positives and false negatives. And the most important cause of false negatives is the PCR inhibitors. In veterinary microbiology, major samples sent for diagnostic purposes and molecular examinations are blood, necropsy materials and stool samples. In this review, the major inhibitory substances in the samples for molecular examinations, their action mechanisms and the methods recommended for the elimination of these substances will be explained

Anahtar Kelimeler

Polymerase Chain Reaction, PCR, PCR inhibitors, elimination methods, veterinary microbiology

Polimeraz Zincir Reaksiyonu İnhibitörleri

Öz

Polimeraz zincir reaksiyonu (PZR), istenilen gen bölgelerinin in-vitro koşullarda kopyalanması, çoğaltılması ve elde edilen ürünlerin gözle görülebilir bir hale getirilerek saptanması prensibine dayanan bir yöntemdir. PZR’ nın hızlı ve primerlerinin özelliğine göre spesifik olması, duyarlılığının yüksek olması gibi avantajlarının yanında bazı dezavantajları da bulunmaktadır. Bunlardan en önemlileri; sonuçların değerlendirilmesinde karşılaşılan güçlükler, yanlış pozitiflikler ve yanlış negatifliklerdir. Yanlış negatifliklerin en önemli sebebi de PZR inhibitörleridir. Veteriner hekimliğinde, başta tanı amaçlı olmak üzere moleküler incelemeler amacıyla sıklıkla incelenen örnekler dışkı, kan ve iç organlardır. Bu derlemede, bahsedilen örnekler içerisinde yer alan inhibitör maddeler, bu maddelerin etki mekanizmaları ve son olarak da bunların eliminasyonu için önerilen yöntemler açıklanacaktır.

Anahtar Kelimeler

Polimeraz Zincir Reaksiyonu, PZR, PZR inhibitörleri, eliminasyon yöntemleri, veteriner mikrobiyolojisi

Kaynakça

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