Objective: Impaired activation of the PI3K/AKT signaling pathway has been associated with tumor development. The PTEN/AKT pathway plays a critical role in cell growth and survival and has been associated with chemotherapy resistance in leukemia. In this study, PTEN and AKT1 gene expression were determined in childhood T-ALL patients, and the effect was investigated on the prognosis of the disease. Materials and Methods: Bone marrow samples from 39 childhood cases were obtained at the time of diagnosis and screened with real-time quantitative PCR for PTEN and AKT1 genes. Results: In our study, no difference between T-ALL patients compared to controls was observed in PTEN gene expression whereas AKT1 gene expression was significantly increased (p<0.0001). There was no significant relationship between gene expression and clinical features of the patients, overall and disease-free survival rates while induction therapy-related survival analyzes revealed a significant relationship between PTEN and AKT1 gene expression and overall survival rates (p=0.026 and p=0.034 Cox regression analysis, respectively). Conclusion: In our study, the presence of increased AKT1 gene expression was demonstrated in T-ALL patients independent of PTEN expression. Future studies will reveal whether it will be a suitable candidate for targeted therapies.
|Destekleyen Kurum||İstanbul Üniversitesi Bilimsel Araştırma Projeleri Birimi ve İstanbul Kalkınma Ajansı, Geleceğe Yatırım: BİYOBANKA projesi|
|Proje Numarası||BAP Proje No: 48185 ve İstanbul Kalkınma Ajansı Proje No: TR10/15/YNK/0093|
|Teşekkür||Hasta materyallerinin sağlanmasındaki katkılarından dolayı Prof. Dr. Zeynep Karakaş ve Prof. Dr. Tiraje Çelkan’a teşekkür ederiz.|
: 31 Ağustos 2020
|APA||Küçükcankurt, F , Hatırnaz Ng, Ö , Sayi̇toğlu, M , Erbi̇lgi̇n, Y . (2020). Çocukluk Çağı T-ALL Hastalarında PTEN ve AKT1 Gen Anlatım Düzeyleri . Sağlık Bilimlerinde İleri Araştırmalar Dergisi , 3 (3) , 114-121 . Retrieved from https://dergipark.org.tr/tr/pub/sabiad/issue/57700/788345|